Calcification stimulates inflammatory signalling pathways in human vascular smooth muscle cells

Proudfoot, Diane; Dautova, Yana; Kozlova, Diana; Epple, Matthias; Bootman, Martin D.

doi:10.1016/j.atherosclerosis.2015.10.078

Cited by 4 publications

(1 citation statement)

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“…All kinds of synthetic molecules and biomolecules can be transported across the cell membrane with the help of calcium phosphate nanoparticles [ 8 ]. The presence of calcium phosphate in human hard tissue renders them inherently biocompatible [ 22 ] unless the nanoparticle dose is very high when it may cause an intracellular disturbance of the calcium level [ 23 – 27 ]. After cellular uptake, calcium phosphate nanoparticles are dissolved in the acidified lysosomes and finally excreted in ionic form [ 28 ].…”

Section: Introductionmentioning

confidence: 99%

Uptake of the proteins HTRA1 and HTRA2 by cells mediated by calcium phosphate nanoparticles

Rotan¹,

Severin²,

Pöpsel³

et al. 2017

Beilstein J. Nanotechnol.

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The efficient intracellular delivery of (bio)molecules into living cells remains a challenge in biomedicine. Many biomolecules and synthetic drugs are not able to cross the cell membrane, which is a problem if an intracellular mode of action is desired, for example, with a nuclear receptor. Calcium phosphate nanoparticles can serve as carriers for small and large biomolecules as well as for synthetic compounds. The nanoparticles were prepared and colloidally stabilized with either polyethyleneimine (PEI; cationic nanoparticles) or carboxymethyl cellulose (CMC; anionic nanoparticles) and loaded with defined amounts of the fluorescently labelled proteins HTRA1, HTRA2, and BSA. The nanoparticles were purified by ultracentrifugation and characterized by dynamic light scattering and scanning electron microscopy. Various cell types (HeLa, MG-63, THP-1, and hMSC) were incubated with fluorescently labelled proteins alone or with protein-loaded cationic and anionic nanoparticles. The cellular uptake was followed by light and fluorescence microscopy, confocal laser scanning microscopy (CLSM), and flow cytometry. All proteins were readily transported into the cells by cationic calcium phosphate nanoparticles. Notably, only HTRA1 was able to penetrate the cell membrane of MG-63 cells in dissolved form. However, the application of endocytosis inhibitors revealed that the uptake pathway was different for dissolved HTRA1 and HTRA1-loaded nanoparticles.

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Section: Introductionmentioning

confidence: 99%

Uptake of the proteins HTRA1 and HTRA2 by cells mediated by calcium phosphate nanoparticles

Rotan¹,

Severin²,

Pöpsel³

et al. 2017

Beilstein J. Nanotechnol.

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Biological and Medical Applications of Calcium Phosphate Nanoparticles

Sokolova

Epple

2021

Chemistry A European J

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Calcium phosphate nanoparticles have ah igh biocompatibility and biodegradability due to their chemical similarity to human hard tissue, for example, bone and teeth. They can be used as efficient carriers for different kinds of biomolecules such as nucleic acids, proteins, peptides, antibodies, or drugs,w hich alone are not able to enter cells where their biological effect is required. They can be loaded with cargom olecules by incorporating them, unlike solid nanoparticles, and also by surface functionalization.This offersp rotection,f or example, against nucleases,a nd the possibility forc ell targeting. If such nanoparticles are functionalized with fluorescing dyes, they can be appliedf or imagingi nv itro and in vivo. Synthesis, functionalization and cell uptake mechanismso fc alcium phosphate nanoparticles are discussed together with applications in transfection, gene silencing, imaging, immunization, and bone substitution. Biodistribution data of calcium phosphate nanoparticles in vivo are reviewed.

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