Calcification rate in <i>Emiliania huxleyi</i> Lohmann in response to light, nitrate and availability of inorganic carbon

Nimer, N. A.; Merrett, M. J.

doi:10.1111/j.1469-8137.1993.tb03776.x

Cited by 90 publications

(72 citation statements)

References 16 publications

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“…In contrast, HCO3 is utilized by high-calcifying cells, and high photosynthetic rate at alkaline pH is sustained by COg released from HCO3" (Nimer & Merrett, 1993). The measurement of carbonate alkalinity during growth confirmed the differences in inorganic carbon utilization between high-and low-calcifying cells.…”

Section: Discussionmentioning

confidence: 56%

“…The low-calcifying strain of E. huxleyi is essentially a 'CO.^ user', because a block in HCO3" utilization occurs either in transport into or within the coccolith vesicle (Nimer & Merrett, 1993) or possibly the chloroplast. In contrast, HCO3 is utilized by high-calcifying cells, and high photosynthetic rate at alkaline pH is sustained by COg released from HCO3" (Nimer & Merrett, 1993).…”

Section: Discussionmentioning

confidence: 99%

“…The addition of eqns (4) and (5) represents the 1 :1 stoichiometry for calcification and photosynthetic CO.^ fixation (eqn 6) observed when HCO3" provides the bulk of exogenous inorganic carbon , 1993.…”

Section: Discussionmentioning

confidence: 99%

“…The production of at a temperature of 15 °C until air-equilibrium of CO2 from HCO3" in calcifying cells sustains high total DIC was obtained. Vitamins and NaNOg were photosyntheticrates(Nimer& Merrett, , 1993, sterilized by Seitz filtration before adding to the and this may be important in oceanic blooms of E. autoclaved medium. Cultures were gently aerated huxleyi in temperate waters because^CO.^ is likely to with air (0-03 % v/v CO^) or COj-free air at a flow be below the K^^.^[CO^ of the cells.…”

Section: Introductionmentioning

confidence: 99%

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Dissolved inorganic carbon utilization in relation to calcite production in Emiliania huxleyi (Lohmann) Kamptner

et al. 1993

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SUMMARYThe relationship between caleite production and dissolved inorganic carbon (DIC) utilization was investigated using high-and low-calcifying strains of Emiliania huxleyi aerated with either air (0-03 % v/v CO^) or COj-free air. With cultures grown on air, calcite production was eight-fold greater in the high-calcifying eulture, but growth rates for high-and low-calcifying cultures were similar. Growth and caleite production were accompanied by a concomitant decrease in DIC and free CO^ in the high-calcifying culture, showing that HCO3" provides inorganic carbon for calcite synthesis. In low-calcifying cultures DIC and free CO.^ were relatively constant, confirming that cells acquire inorganic carbon mainly by the diffusive entry of free CO,.When cultures were aerated with CO^-free air the free CO^ concentration was below tbe /^^^[COJ for high-and low-calcifying cells and the low-calcifying cells were unable to grow. Growth of high-calcifying cells was observed, and the calcite yield was little changed from cultures gassed with air (003 °o v/v CO^). At tbese minimal COj eoncentrations HCO3" may provide inorganic carbon for calcite synthesis and CO^ for photosynthesis, allowing growth to oeeur. Calcite synthesis by E. huxleyi decreases DIC and carbonate alkalinity in cultures not in equilibrium with the gas phase.

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Section: Discussionmentioning

confidence: 56%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Dissolved inorganic carbon utilization in relation to calcite production in Emiliania huxleyi (Lohmann) Kamptner

et al. 1993

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“…The wide availability of physiologically well characterized strains of calcifying phytoplankton in long-term culture enabled the impact of ocean acidification to be tested. Early laboratory experiments carried out on the most abundant modern species of coccolithophorid, Emiliania huxleyi, in which the pH of the growth medium was decreased by addition of acid, produced varying calcification responses (Buitenhuis et al, 1999;Nimer and Merrett, 1993). However, the chemical conditions used in these manipulations often deviated substantially from either modern or possible future geochemistry.…”

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confidence: 99%

From laboratory manipulations to Earth system models: scaling calcification impacts of ocean acidification

et al. 2009

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Abstract. The observed variation in the calcification responses of coccolithophores to changes in carbonate chemistry paints a highly incoherent picture, particularly for the most commonly cultured "species", Emiliania huxleyi. The disparity between magnitude and potentially even sign of the calcification change under simulated end-of-century ocean surface chemical changes (higher pCO 2 , lower pH and carbonate saturation), raises challenges to quantifying future carbon cycle impacts and feedbacks because it introduces significant uncertainty in parameterizations used for global models. Here we compile the results of coccolithophore carbonate chemistry manipulation experiments and review how ocean carbon cycle models have attempted to bridge the gap from experiments to global impacts. Although we can rule out methodological differences in how carbonate chemistry is altered as introducing an experimental bias, the absence of a consistent calcification response implies that model parameterizations based on small and differing subsets of experimental observations will lead to varying estimates for the global carbon cycle impacts of ocean acidification. We highlight two pertinent observations that might help: (1) the degree of coccolith calcification varies substantially, both between species and within species across different genotypes, and (2) the calcification response across mesocosm and shipboard incubations has so-far been found to be relatively consistent. By analogy to descriptions of plankton growth rate vs. temperature, such as the "Eppley curve", Correspondence to: A. Ridgwell (andy@seao2.org) which seek to encapsulate the net community response via progressive assemblage change rather than the response of any single species, we posit that progressive future ocean acidification may drive a transition in dominance from more to less heavily calcified coccolithophores. Assemblage shift may be more important to integrated community calcification response than species-specific response, highlighting the importance of whole community manipulation experiments to models in the absence of a complete physiological understanding of the underlying calcification process. However, on a century time-scale, regardless of the parameterization adopted, the atmospheric pCO 2 impact of ocean acidification is minor compared to other global carbon cycle feedbacks.

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Inorganic Carbon Acquisition by Aquatic Primary Producers

Rokitta¹,

Kranz²,

Rost³

2022

Blue Planet, Red and Green Photosynthesis

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Calcification rate in Emiliania huxleyi Lohmann in response to light, nitrate and availability of inorganic carbon

Cited by 90 publications

References 16 publications

Dissolved inorganic carbon utilization in relation to calcite production in Emiliania huxleyi (Lohmann) Kamptner

Dissolved inorganic carbon utilization in relation to calcite production in Emiliania huxleyi (Lohmann) Kamptner

From laboratory manipulations to Earth system models: scaling calcification impacts of ocean acidification

Inorganic Carbon Acquisition by Aquatic Primary Producers

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