Calcification on cultured human dental pulp cells exposed to high glucose level

Okamura, Tomoharu; Nishikawa, Tetsunari; Tanaka, Akio

doi:10.3353/omp.13.47

Cited by 3 publications

(3 citation statements)

References 23 publications

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“…However, the inhibitory effect of HG on cell proliferation was detected on day 9 and day 11. In contrast, Okamura et al (18) reported the positive effects of glucose after HDPCs were cultured in high glucose medium for 2, 4 and 7 days. Kanafi et al (19) also found that high glucose increased proliferation of HDPCs from both primary and permanent teeth.…”

Section: Discussionmentioning

confidence: 91%

“…Previous studies have explored the effects of glucose on odonto/osteogenic differentiation of HDPCs but the results were still controversial. Okamura et al (18) demonstrated that high glucose increased odontogenic differentiation of HDPCs. However, a study by Kanafi et al (19) revealed no differences among glucose concentrations on mineralization of HDPCs.…”

Section: Discussionmentioning

confidence: 99%

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High Glucose Affects Proliferation, Reactive Oxygen Species and Mineralization of Human Dental Pulp Cells

et al. 2020

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Diabetes is a group of metabolic disorders that can lead to damage and dysfunction of many organs including the dental pulp. Increased inflammatory response, reduction of dentin formation and impaired healing were reported in diabetic dental pulp. Hyperglycemia, which is a main characteristic of diabetes, was suggested to play a role in many diabetic complications. Therefore our aim was to investigate the effects of high glucose levels on proliferation, reactive oxygen species (ROS) production and odontogenic differentiation of human dental pulp cells (HDPCs). HDPCs were cultured under low glucose (5.5mM Glucose), high glucose (25 mM Glucose) and mannitol (iso-osmolar control) conditions. Cell proliferation was analyzed by MTT assay for 11 days. Glutathione and DCFH-DA assay were used to assess ROS and antioxidant levels after 24 h of glucose exposure. Odontogenic differentiation was evaluated and quantified by alizarin red staining on day 21. Expression of mineralization-associated genes, which were alkaline phosphatase, dentin sialophosphoprotein and osteonectin, was determined by RT-qPCR on day 14. The results showed that high glucose concentration decreased proliferation of HDPCs. Odontogenic differentiation, both by gene expression and mineral matrix deposit, was inhibited by high glucose condition. In addition, high DCF levels and low reduced glutathione levels were observed in high glucose condition. However, no differences were observed between mannitol and low glucose conditions. In conclusion, the results clearly showed the negative effect of high glucose condition on HDPCs proliferation and differentiation. Moreover, it also induced ROS production of HDPCs.

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Section: Discussionmentioning

confidence: 91%

Section: Discussionmentioning

confidence: 99%

High Glucose Affects Proliferation, Reactive Oxygen Species and Mineralization of Human Dental Pulp Cells

et al. 2020

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“…However, to our knowledge, no study to date has investigated the interaction between change in NO after tooth extraction and angiogenesis or lymphangiogenesis in a diabetes model. Reports on how diabetes affects bone formation differ: it has been reported to affect bone formation due to impairment of osteoblasts 6) ; diabetic hyperglycemia induced osteogenesis 21) ; and no influence of diabetes was observed on bone healing in extraction sockets 23) . This indicates that Akita mice may offer a useful means of clarifying the effect of diabetes on bone formation.…”

Section: Original Articlementioning

confidence: 99%

Lymphangiogenesis and NOS Localization in Healing Process after Tooth Extraction in Akita Mouse

Takahashi¹,

Kikuchi

Ambe

et al. 2016

Bull. Tokyo Dent. Coll.

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Type I diabetes, an autoimmune disease, induces insulin deficiency, which then disrupts vascular endothelial cell function, affecting blood and lymphatic vessels. Nitric oxide (NO) is an immune-induced destructive mediator in type I diabetes, and inhibition of its production promotes arteriosclerosis. In this study, lymphangiogenesis and expression of NO synthase (NOS) during the healing process after tooth extraction were investigated immunohistochemically in control (C57BL) and Akita mice as a diabetes model. Between 1, 4, and 10 days after extraction, expression of NOS, vascular endothelial growth factor-C (VEGF-C), VEGF receptor-3 (VEGFR-3), and von Willebrand factor was strongest during the granulation tissue phase. This suggests that severe inflammation triggers regulation of NOS and these other angiogenic and lymphangiogenic factors. During the callus phase, a few days after extraction, induced osteoblasts were positive for VEGF-C and VEGFR-3 in both the control and Akita mice, suggesting that bone formation is active in this period. Bone formation in the Akita group exceeded that in the controls. Bone tissue formation was disrupted under hyperglycemic conditions, however, suggesting that such activity would be insufficient to produce new bone.

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Epigenetic changes underlie the association between diabetes mellitus and oral diseases

et al. 2023

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Calcification on cultured human dental pulp cells exposed to high glucose level

Cited by 3 publications

References 23 publications

High Glucose Affects Proliferation, Reactive Oxygen Species and Mineralization of Human Dental Pulp Cells

High Glucose Affects Proliferation, Reactive Oxygen Species and Mineralization of Human Dental Pulp Cells

Lymphangiogenesis and NOS Localization in Healing Process after Tooth Extraction in Akita Mouse

Epigenetic changes underlie the association between diabetes mellitus and oral diseases

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