Calcification of Selected Strains of
            <i>Streptococcus mutans</i>
            and
            <i>Streptococcus sanguis</i>

Streckfuss, J.L.; Smith, W. N.; Brown, Lee R.; Campbell, Marion M.

doi:10.1128/jb.120.1.502-506.1974

Cited by 46 publications

(12 citation statements)

References 16 publications

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“…Previous studies have similarly shown the precipitation of calcium phosphate by bacteria when calcium was provided to the growth medium (e.g. Streckfuss et al, 1974;Lucas & Prevot, 1984;Hirschler et al, 1990;Moorer et al, 1993;Ohara et al, 2002). In our study, no calcium phosphate crystal larger than approximately 100 nm was observed outside the cells.…”

Section: Calcium Phosphate Precipitation In Caulobacter Crescentusmentioning

confidence: 83%

Scanning transmission X‐ray microscopy study of microbial calcification

et al. 2004

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Calcium phosphates and calcium carbonates are among the most prevalent minerals involved in microbial fossilization. Characterization of both the organic and mineral components in biomineralized samples is, however, usually difficult at the appropriate spatial resolution (i.e. at the submicrometer scale). Scanning transmission X‐ray microscopy (STXM) was used to measure C K‐edge, P L‐edge, and Ca L‐edge near‐edge X‐ray absorption fine structure (NEXAFS) spectra of some calcium‐containing minerals common in biomineralization processes and to study the experimental biomineralization by the model microorganism, Caulobacter crescentus. We show that the Ca L2,3‐edges for hydroxyapatite, calcite, vaterite, and aragonite are unique and can be used as probes to detect these different mineral phases. Using these results, we showed that C. crescentus cells, when cultured in the presence of high calcium concentration, precipitated carbonate hydroxyapatite. In parallel, we detected proteins, polysaccharides, and nucleic acids in the mineralizing bacteria at the single‐cell scale. Finally, we discussed the utility of STXM for the study of natural fossilized microbial systems.

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Section: Calcium Phosphate Precipitation In Caulobacter Crescentusmentioning

confidence: 83%

Scanning transmission X‐ray microscopy study of microbial calcification

et al. 2004

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“…Periapical bacterial plaque may calcify in the absence of a sinus tract, the minerals being furnished by periapical inflammatory exudates (Sunde et al 2002). Some bacterial species have also been shown to have the ability to form intracellular apatite crystals (Streckfuss et al 1974).…”

Section: Discussionmentioning

confidence: 99%

Calculus‐like deposit on the apical external root surface of teeth with post‐treatment apical periodontitis: report of two cases

Ricucci¹,

Martorano²,

Bate

et al. 2005

Int Endodontic J

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“…The localization (intra-vs. extracellular) of the alkaline phosphatase therefore has an influence on the calcification patterns in E. coli, and could partly explain differences in the patterns of Ca-phosphate precipitation among different bacterial species as described for instance by Rizzo et al (1963), Ennever et al (1981), and Streckfuss et al (1974). However, it is likely that calcification patterns are also influenced by the composition (and in particular, saturation) of the medium.…”

Section: Relation Between Bacterial Calcification Patterns and Alkalimentioning

confidence: 99%

Calcium-Phosphate Biomineralization Induced by Alkaline Phosphatase Activity in Escherichia coli: Localization, Kinetics, and Potential Signatures in the Fossil Record

et al. 2015

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International audienceBacteria are thought to play an important role in the formation of calcium-phosphate minerals composing marine phosphorites, as supported by the common occurrence of fossil microbes in these rocks. Phosphatase enzymes may play a key role in this process. Indeed, they may increase the supersaturation with respect to Ca-phosphates by releasing orthophosphate ions following hydrolysis of organic phosphorus. However, several questions remain unanswered about the cellular-level mechanisms involved in this model, and its potential signatures in the mineral products. We studied Ca-phosphate precipitation by different strains of Escherichia coli which were genetically modified to differ in the abundance and cellular localization of the alkaline phosphatase (PHO A) produced. The mineral precipitated by either E. coli or purified PHO A was invariably identified as a carbonate-free non-stoichiometric hydroxyapatite. However, the bacterial precipitates could be discriminated from the ones formed by purified PHO A at the nano-scale. PHO A localization was shown to influence the pattern of Ca-phosphate nucleation and growth. Finally, the rate of calcification was proved to be consistent with the PHO A enzyme kinetics. Overall, this study provides mechanistic keys to better understand phosphogenesis in the environment, and experimental references to better interpret the microbial fossil record in phosphorites

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Calcification of Selected Strains of Streptococcus mutans and Streptococcus sanguis

Cited by 46 publications

References 16 publications

Scanning transmission X‐ray microscopy study of microbial calcification

Scanning transmission X‐ray microscopy study of microbial calcification

Calculus‐like deposit on the apical external root surface of teeth with post‐treatment apical periodontitis: report of two cases

Calcium-Phosphate Biomineralization Induced by Alkaline Phosphatase Activity in Escherichia coli: Localization, Kinetics, and Potential Signatures in the Fossil Record

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