Calcification of Pulp Canal Space after Replantation of Immature Rat Molars

Abstract

The objective of this study was to investigate the process of hard tissue formation after replantation of open-ended immature teeth in rat and morphologically type the hard tissue generated according to its degree of calcification. The pulp chamber, periapical cells and matrix were observed by light microscopy. Degree of ossification in undecalcified, polished samples was determined by contact microradiography (CMR). After tooth replantation, pulp cells and odontoblasts temporarily showed degeneration and necrosis. Thereafter, some of the surviving pulp cells showed activation, with healing and revascularization of apical tissue, proliferating to form matrix. These results together with the CMR findings showed that bone-like dentin was formed in the cervical pulp chamber, and that intracanal cementogenesis occurred in the apical area, continuing until the pulp chamber was filled with calcified tissues.

Ultrastructural study of tissues surrounding replanted teeth and dental implants

“…Tooth replantation was done with a method as previously described (Ihara et al 2007). Briefly, under general anesthesia with ketamine hydrochloride (125 mg/kg), upper right molars were luxated with a dental excavator and carefully extracted with forceps in order to avoid damaging surrounding tissues.…”

Ultrastructure of Dentogingival Border of Normal and Replanted Tooth and Dental Implant

“…Experiment I: Tooth replantation was performed by a previously described method (Ihara et al, 2007). Briefly, under general anesthesia with ketamine hydrochloride, upper right molars were luxated with a dental excavator and carefully extracted with forceps in order to avoid damaging surrounding tissues.…”