Calcification of bovine pericardium: Glutaraldehyde versus no-react biomodification

Abolhoda, Amir; Yu, Shaw L.; Oyarzun, J. Rodrigo; McCormick, John R.; Bogden, John D.; Gabbay, Shlomo

doi:10.1016/0003-4975(96)00277-9

Cited by 68 publications

(41 citation statements)

References 8 publications

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“…Thereby, the prosthesis remains structurally intact for a longer period of time compared with non-cross-linked materials. 16,17 The potential benefits of biologic materials include infection tolerance, host tissue ingrowth, vascularization, and lesser adhesion formation than that seen with synthetic prostheses. Downsides include higher cost, and in theory, potential disease transmission.…”

Section: Introductionmentioning

confidence: 98%

Experimental Evaluation of Four Biologic Prostheses for Ventral Hernia Repair

Gaertner

Bonsack

Delaney

2007

Journal of Gastrointestinal Surgery

139

103

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Section: Introductionmentioning

confidence: 98%

Experimental Evaluation of Four Biologic Prostheses for Ventral Hernia Repair

Gaertner

Bonsack

Delaney

2007

Journal of Gastrointestinal Surgery

139

103

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“…Als weiterer negativer Effekt der chemischen Behandlung ist zu verzeichnen, dass es nicht nur zu einem kompletten Verlust der Endothelzellen auf der Oberfläche kommt, sondern auch das Wachstum von Zellen aus der Umgebung auf das Biomaterial weitgehend verhindert wird [4,8]. Den Implantaten fehlt somit die Barrierefunktion vitaler Zellen, wodurch das Gewebe für biochemische Prozesse und entzündlich-immunologische Abläufe anfälliger ist [1,9,10,16,23].…”

Section: Introductionunclassified

“…Auch durch Modifikationen der chemischen Vorbehandlung konnte die Anlagerung von Calcium nicht verhindert werden [1,10,16]: So bedingte die Vorbehandlung mit Formaldehyd ausgeprägte Entzündungs-reaktionen und erforderte einen noch früheren Austausch der Klappen [21]. Dies gilt im Wesentlichen auch für Änderungen der Glutaraldehydbehandlung, welche die Degeneration und Kalzifizierung der Bioimplantate ebenfalls nicht verhindern konnten [15,17,18].…”

Section: Introductionunclassified

“…In neuester Zeit wurden sog. Detoxifikationsverfahren entwickelt, die aber die Verkalkungsneigung der Implantate nur reduzierten [1,10].…”

Section: Introductionunclassified

“…Ursächlich hierfür ist, dass die aldehydgelagerten Biomaterialien zytotoxische Stoffe freisetzen, die zum Untergang der Zellen führen und damit eine homogene Oberflächenbedeckung verhindern [4,8]. Andererseits kann derzeit auf die Glutaraldehydbehandlung nicht verzichtet werden, da sie die Stabilität des Materials sicherstellt [1].…”

Section: Introductionunclassified

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Kultivierung humaner Zellen auf polymerbeschichteten Bioimplantaten?ein neues Konzept zur Verbesserung der Implantateigenschaften

Pierkes

Chang

Alt

et al. 2004

Herz

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Cytotoxicity of PVPAC‐treated bovine pericardium: A potential replacement for glutaraldehyde in biological heart valves

et al. 2013

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Acellular biological tissues, including bovine pericardium (BP), have been proposed as biomaterial for tissue engineering. BP is usually modified chemically to improve mechanical and biological properties using glutaraldehyde, the standard reagent for preservation of fresh bioprosthetic materials. Glutaraldehyde-fixed BP (Glut-BP), the most widely used material in heart valve manufacture, has been associated with calcification in vivo. In an attempt to reduce this issue and maintain its biocompatibility, this study assesses the physical properties and cytotoxicity of lyophilized BP treated with poly (vinylpyrrolidone-co-acrolein) (PVPAC-BP), a novel copolymer, as a substitute for glutaraldehyde. For that, PVPAC-BP surface ultrastructure, elastic function, water uptake and tissue calcification were evaluated. For the analysis of biocompatibility, fibroblasts (3T3-L1) and endothelial cells (HUVEC) were cultured on PVPAC-BP, Untreated-BP and Glut-BP. Nitric oxide (NO) release assay, fluorescence and SEM images of endothelial cells adhered on scaffolds were also performed. As results, the data show some advantages of PVPAC-BP over the Glut-BP. The PVPAC-BP maintains partially the original ultrastructure and elastic properties, improves scaffold hydration, and presents less calcium phosphate deposits. The cells demonstrated strong attachment, high proliferation rate, and formation of a monolayer on PVPAC-BP. Attached cells were also able to release NO de-monstrating regular metabolism. In conclusion, PVPAC may be considered as a promising alternative to BP treatment improving the efficiency of cell attachment and proliferation and also avoid immunogenicity.

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Calcification of bovine pericardium: Glutaraldehyde versus no-react biomodification

Cited by 68 publications

References 8 publications

Experimental Evaluation of Four Biologic Prostheses for Ventral Hernia Repair

Experimental Evaluation of Four Biologic Prostheses for Ventral Hernia Repair

Kultivierung humaner Zellen auf polymerbeschichteten Bioimplantaten?ein neues Konzept zur Verbesserung der Implantateigenschaften

Cytotoxicity of PVPAC‐treated bovine pericardium: A potential replacement for glutaraldehyde in biological heart valves

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