Calcification of a Cariogenic Streptococcus and of Corynebacterium (Bacterionema) matruchotii

Moorer, W.R.; Cate, J.M. ten; Buijs, J.F.

doi:10.1177/00220345930720060501

Cited by 26 publications

(12 citation statements)

References 20 publications

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“…The major taxon in these samples was identified as Corynebacterium , a Gram-positive, facultatively anaerobe bacterium that resembles Gram-negatives with respect to the lipid layer in the cell membrane (John, 1984). Corynebacterium is associated with mature plaque and is found in dental calculus (Moorer et al, 1993). …”

Section: Discussionmentioning

confidence: 99%

Effect of an oxygenating agent on oral bacteria in vitro and on dental plaque composition in healthy young adults

Mostajo

Reijden

Buijs

et al. 2014

Front. Cell. Infect. Microbiol.

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Oral bacteria live in symbiosis with the host. Therefore, when mouthwashes are indicated, selective inhibition of taxa contributing to disease is preferred instead of broad-spectrum antimicrobials. The potential selectivity of an oxygenating mouthwash, Ardox-X® (AX), has not been assessed. The aim of this study was to determine the antimicrobial potential of AX and the effects of a twice-daily oral rinse on dental plaque composition.Material and methods: In vitro, 16 oral bacterial strains were tested using agar diffusion susceptibility, minimum inhibitory and minimum bactericidal concentration tests. A pilot clinical study was performed with 25 healthy volunteers. Clinical assessments and microbiological sampling of supragingival plaque were performed at 1 month before the experiment (Pre-exp), at the start of the experiment (Baseline) and after the one-week experimental period (Post-exp). During the experiment individuals used AX mouthwash twice daily in absence of other oral hygiene measures. The microbiological composition of plaque was assessed by 16S rRNA gene amplicon sequencing.Results: AX showed high inter-species variation in microbial growth inhibition. The tested Prevotella strains and Fusobacterium nucleatum showed the highest sensitivity, while streptococci and Lactobacillus acidophilus were most resistant to AX. Plaque scores at Pre-exp and Baseline visits did not differ significantly (p = 0.193), nor did the microbial composition of plaque. During a period of 7-days non-brushing but twice daily rinsing plaque scores increased from 2.21 (0.31) at Baseline to 2.43 (0.39) Post-exp. A significant microbial shift in composition was observed: genus Streptococcus and Veillonella increased while Corynebacterium, Haemophilus, Leptotrichia, Cardiobacterium and Capnocytophaga decreased (p ≤ 0.001).Conclusion: AX has the potential for selective inhibition of oral bacteria. The shift in oral microbiome after 1 week of rinsing deserves further research.

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Section: Discussionmentioning

confidence: 99%

Effect of an oxygenating agent on oral bacteria in vitro and on dental plaque composition in healthy young adults

Mostajo

Reijden

Buijs

et al. 2014

Front. Cell. Infect. Microbiol.

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“…All cultures were maintained in a 150 cm 2 tissue culture flask containing 10 mL of Dulbecco's Minimal Essential Media supplemented with triple-filtered 10% fetal calf serum, 50 μmol/L β-mercaptoethanol, and 3.6 mmol/L CaCl 2 . After 3 weeks, the calcified nanoparticles were washed five times with physiologic buffered saline solution (PBS at pH 7.4), and then the nanoparticles were harvested with a rubber cell-scraper and pooled together in 1.5 mL microtubes for washing, such that each wash consisted of 1 mL PBS centrifugation at 15,000 g for 10 minutes.…”

Section: Methodsmentioning

confidence: 99%

Search for Microbial Signatures within Human and Microbial Calcifications Using Soft X-Ray Spectromicroscopy

Benzerara

Miller

Barell

et al. 2006

Journal of Investigative Medicine

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“…Recent clinical studies have found strains of S. mitis and S. oralis (1,11), S. cristatus (1,11,42), and C. gingivalis and C. rectus (1) to be associated with oral health. C. matruchotii is associated with calculus formation (34), which occurs at high pH. S. gordonii has been associated with sound surfaces in a study of nursing bottle caries (31) and has been found to reduce the effects of many S. mutans virulence factors (26,52).…”

Section: Vol 48 2010 Sequence Analysis Of Severe Caries In Permanenmentioning

confidence: 99%

Bacterial 16S Sequence Analysis of Severe Caries in Young Permanent Teeth

et al. 2010

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Previous studies have confirmed the association of the acid producers Streptococcus mutans and Lactobacillus spp. with childhood caries, but they also suggested these microorganisms are not sufficient to explain all cases of caries. In addition, health-associated bacterial community profiles are not well understood, including the importance of base production and acid catabolism in pH homeostasis. The bacterial community composition in health and in severe caries of the young permanent dentition was compared using Sanger sequencing of the ribosomal 16S rRNA genes. Lactobacillus species were dominant in severe caries, and levels rose significantly as caries progressed from initial to deep lesions. S. mutans was often observed at high levels in the early stages of caries but also in some healthy subjects and was not statistically significantly associated with caries progression in the overall model. Lactobacillus or S. mutans was found either at low levels or not present in several samples. Other potential acid producers observed at high levels in these subjects included strains of Selenomonas, Neisseria, and Streptococcus mitis. Propionibacterium FMA5 was significantly associated with caries progression but was not found at high levels. An overall loss of community diversity occurred as caries progressed, and species that significantly decreased included the Streptococcus mitis-S. pneumoniae-S. infantis group, Corynebacterium matruchotii, Streptococcus gordonii, Streptococcus cristatus, Capnocytophaga gingivalis, Eubacterium IR009, Campylobacter rectus, and Lachnospiraceae sp. C1. The relationship of acid-base metabolism to 16S rRNA gene-based species assignments appears to be complex, and metagenomic approaches that would allow functional profiling of entire genomes will be helpful in elucidating the microbial pathogenesis of caries.Dental caries is the most common chronic disease of childhood, affecting nearly three-fourths of all children by the age of 17 years (50). The majority of children experience mild caries in the permanent dentition that is easily managed, but nearly 20% of children suffer more aggressive caries (19) that is destructive and often recurrent. The cariogenicity of Streptococcus mutans and Lactobacillus species in tooth-associated biofilms has long been established based on culture studies (51), but this approach has provided a limited ability to study the role of other species present in biofilm communities. Recently DNA-based methods have been used to study early childhood caries (5, 11, 23), caries of the primary and permanent teeth in children and young adults (1), root caries in the elderly (42), and advanced dentin lesions (8,10,36). Taken together these studies have confirmed the association of S. mutans and Lactobacillus species with childhood caries, but they also suggest that these species are not sufficient to explain all cases of caries. In addition, health-associated bacterial community profiles are not well understood, including the importance of species that produce basic co...

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Calcification of a Cariogenic Streptococcus and of Corynebacterium (Bacterionema) matruchotii

Cited by 26 publications

References 20 publications

Effect of an oxygenating agent on oral bacteria in vitro and on dental plaque composition in healthy young adults

Effect of an oxygenating agent on oral bacteria in vitro and on dental plaque composition in healthy young adults

Search for Microbial Signatures within Human and Microbial Calcifications Using Soft X-Ray Spectromicroscopy

Bacterial 16S Sequence Analysis of Severe Caries in Young Permanent Teeth

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