Calcification and Bone Induction Studies in Heterogeneous Phosphorylated Hydrogels

Swart, J.G.N.; Driessen, A. A.; deVisser, A.

doi:10.1021/bk-1976-0031.ch011

Cited by 12 publications

(5 citation statements)

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“…The effect of negatively charged functional groups such as carboxylate and phosphate on the calcification of polymeric materials has recently been the subject of an excellent review by Chirila and Zainuddin . Although the incorporation of phosphorus-containing moieties has been shown to lead, in vitro, to the enhanced calcification of naturally occurring materials such as cotton, , bamboo, , and chitin, the calcification results for synthetic polymers is less well-studied and somewhat controversial . As Chirila pointed out in his review “the effect of the phosphate group may be more complicated than initially thought”.…”

Section: Resultsmentioning

confidence: 99%

Synthesis of Soluble Phosphate Polymers by RAFT and Their in Vitro Mineralization.

et al. 2006

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Soluble linear (non-cross-linked) poly(monoacryloxyethyl phosphate) (PMAEP) and poly(2-(methacryloyloxy)ethyl phosphate) (PMOEP) were successfully synthesized through reversible addition-fragmentation chain transfer (RAFT)-mediated polymerization and by keeping the molecular weight below 20 K. Above this molecular weight, insoluble (cross-linked) polymers were observed, postulated to be due to residual diene (cross-linkable) monomers formed during purification of the monomers, MOEP and MAEP. Block copolymers consisting of PMAEP or PMOEP and poly(2-(acetoacetoxy)ethyl methacrylate) (PAAEMA) were successfully prepared and were immobilized on aminated slides. Simulated body fluid studies revealed that calcium phosphate (CaP) minerals formed on both the soluble polymers and the cross-linked gels were very similar. Both the PMAEP polymers and the PMOEP gel showed a CaP layer most probably brushite or monetite based on the Ca/P ratios. A secondary CaP mineral growth with a typical hydroxyapatite (HAP) globular morphology was found on the PMOEP gel. The soluble PMOEP film formed carbonated HAP according to Fourier transform infrared (FTIR) spectroscopy. Block copolymers attached to aminated slides showed only patchy mineralization, possibly due to the ionic interaction of negatively charged phosphate groups and protonated amines.

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Section: Resultsmentioning

confidence: 99%

Synthesis of Soluble Phosphate Polymers by RAFT and Their in Vitro Mineralization.

et al. 2006

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“…In bone, molecules such as bone sialoprotein or other phosphoproteins have been identified as suitable candidates for the nucleation of hydroxyapatite 7, 16. Phosphate‐containing polymers have been proposed as calcium‐attracting materials, capable of inducing biomineralization when immersed in synthetic physiological fluids (due to the calcium‐capturing properties of these negatively charged groups) 17, 18. It was demonstrated that carboxylic residues of some acidic proteins (e.g., bone sialoprotein) or carboxylic pending groups of polymers can also act as mineralization nucleators 8, 16, 19.…”

Section: Discussionmentioning

confidence: 99%

“…However, they have used nonphysiologic conditions to obtain HA deposition (incubation at temperatures in the range of 37 to 80°C) 9. In a similar way, phosphorylated‐pHEMA was proposed several years ago by Swart et al, as a bone substitute 17. The polymer was prepared by reacting HEMA and phosphorus pentoxide.…”

Section: Discussionmentioning

confidence: 99%

The in vivo calcification capacity of a copolymer, based on methacryloyloxyethyl phosphate, does not favor osteoconduction

Stancu

Filmon

Grizon

et al. 2004

J Biomedical Materials Res

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Polymers can be interesting alternatives to bone grafts; they must present suitable mechanical and osteoconductive properties. Biomimetic properties may be a key factor for the recognition by bone cells. Methacryloyloxyethyl phosphate (MOEP) was found to enhance hydroxyapatite deposition. The copolymer containing MOEP and 1-vinyl-2-pyrrolidinone (50-50%) binds large amounts of calcium. Particles of the copolymer were used to fill large cranial bone defects in the rat. After a 12-week healing period, the animals were euthanized and the skulls examined by X-ray, histology, and electron microscopy (EM). The high phosphate content of the polymer conferred a marked calcium-binding capacity, and the particles were heavily calcified. They were embedded in a light fibrous stroma containing numerous capillaries and multinucleated giant cells. The osteoconductive properties were poor: only few trabeculae developed centripetally from the margins of the defects. There was no bone bonding and no osteoblast on the surface of the calcified material. Backscattered EM revealed that the degree of calcification was homogeneous in all particles. Calcium-phosphorus calcospherites were never observed. The material appeared to trap calcium but to impair nucleation because only small hydroxyapatite tablets were occasionally observed. Polyphosphated materials do not represent a suitable source of potentially usable bone substitutes.

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“…Substrates' rinsing with piranha solution (prepared using 75% concentrated H 2 SO 4 and 25% of H 2 O 2 30%) should be performed for approximately 1 minute, followed by distilled water cleaning and drying under a nitrogen stream; this treatment should immediately precede the use. (Recommended time for rinsing with piranha is up to 5 minutes, in order to remove the contaminants and to avoid the increase of the superficial roughness (Ulman, 1991). Thiols.…”

Section: Methodsmentioning

confidence: 99%

“…Mineralization to occur needs nucleation to be induced by a perturbation like the ions' capturing by special molecules/species. Based on the observation that natural phosphate-containing proteins are actively involved in naturally occurring mineralization and due to their calcium affinity, phosphatecontaining polymers have been studied with respect to hydroxyapatite formation in physiological fluids (Stancu et al, 2004;Swart et al, 1976). The literature also presents the carboxylic functionalities from bone proteins (e.g.…”

Section: Biomineralization Inductive Materialsmentioning

confidence: 99%

SPR Imaging Label-Free Control of Biomineral Nucleation!?

Izabela-Cristina¹

2010

Intelligent and Biosensors

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Calcification and Bone Induction Studies in Heterogeneous Phosphorylated Hydrogels

Cited by 12 publications

References 0 publications

Synthesis of Soluble Phosphate Polymers by RAFT and Their in Vitro Mineralization.

Synthesis of Soluble Phosphate Polymers by RAFT and Their in Vitro Mineralization.

The in vivo calcification capacity of a copolymer, based on methacryloyloxyethyl phosphate, does not favor osteoconduction

SPR Imaging Label-Free Control of Biomineral Nucleation!?

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