Calbindin-D9k mRNA is tightly regulated during the estrous cycle in the rat uterus

Krisinger, John; Dann, Jamie L.; Currie, William D.; Jeung, Eui‐Bae; Leung, Peter C.K.

doi:10.1016/0303-7207(92)90182-6

Cited by 56 publications

(51 citation statements)

References 17 publications

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“…CaBP-9k and CaBP-28k are essential factors for early embryo implantation [16][17][18] and are affected by the sex steroid hormones E2 and P4. CaBP-9k expression is increased by E2 and P4 at estrus in rats but unaffected at diestrus in mice [1,11]. TRPVs showed similar expression patterns with E2 and/or P4 treat- ment.…”

Section: Discussionmentioning

confidence: 88%

“…These actions of steroid hormones correspond with widespread distribution in various organs of ER and PR, which are members of the steroid receptor superfamily and act as an important regulator of gene transcription and cell signaling [2,4,12,15,23]. On the other hand, the duodenum and kidney are important target tissues for steroid hormone actions involved in gene expressions and calcium homeostasis [7,9,25].Expression of TRPV6 [formerly named calcium transport protein subtype 1 (CaT1) or epithelial calcium channel 2 (ECaC2)] [21] has been found in the duodenum, kidney and exocrine tissues such as the pancreas, prostate, placenta and uterus [6,11,19,21,27,30]. Duodenal and renal TRPV6 expression is known to be regulated by vitamin D, dietary calcium, and estrogen.…”

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confidence: 99%

“…Expression of TRPV6 [formerly named calcium transport protein subtype 1 (CaT1) or epithelial calcium channel 2 (ECaC2)] [21] has been found in the duodenum, kidney and exocrine tissues such as the pancreas, prostate, placenta and uterus [6,11,19,21,27,30]. Duodenal and renal TRPV6 expression is known to be regulated by vitamin D, dietary calcium, and estrogen.…”

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confidence: 99%

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Duodenal and Renal Transient Receptor Potential Vanilloid 6 Is Regulated by Sex Steroid Hormones, Estrogen and Progesterone, in Immature Rats

Jung

Kim

Yang

et al. 2011

The Journal of Veterinary Medical Science

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ABSTRACT. Transient receptor potential vanilloid 6 (TRPV6) is a major calcium channel located in the apical and basolateral membranes of cells and distributed widely in many other organs, especially the exocrine tissues, intestine and uterus. Uterine TRPV6 is affected by sex steroid hormonal changes during the estrous cycle and pregnancy. To determine whether sex steroid hormones influence the expression of TRPV6 in other tissues, we further examined TRPV6 mRNA expression in the duodenum and kidney by reverse transcriptase and real-time PCRs and its protein levels by Western blot analysis and immunohistochemistry (IHC). Immature rats were injected with 17-estradiol (E2; 40 g/kg) and/or progesterone (P4; 4 mg/kg) for three days. Treatment with a mixture of E2 and P4 (E2/P4) increased TRPV6 expression in the duodenum, and this increase was maintained when the rats were treated with receptor antagonists of estrogen (ICI 182,780; 10 mg/kg) or progesterone (RU 486; 10 mg/kg) 30 min before E2/P4 injection. However, no difference was observed in renal TRPV6 protein levels following the treatment with sex steroid hormones and/or antagonists by Western blot analysis and IHC. These results suggest that duodenal TRPV6 expression may be induced by E2 combined with P4, whereas the renal TRPV6 level is unaffected by these steroids.KEY WORDS: duodenum, immature rats, kidney, transient receptor potential vanilloid 6 (TRPV6).J. Vet. Med. Sci. 73(6): 711-716, 2011 Calcium, the most abundant cation ion in the human body, is involved in organizing the bones and teeth and working in the extra-and intracellular fluids. As a universal intracellular messenger, calcium ion modulates neurotransmission, muscle constructions and secretion. Calcium ion enters the cell through the tight junctions between epithelial cells or through the apical and basolateral cell membranes. Calcium ion removal from epithelial cells requires apical calcium entry channels, including the intracellular calcium binding protein calbindin-D 9k , and the calcium pump plasma membrane calcium ATPase (PMCA1b) [5,10,13]. Transcellular calcium transport occurs through apical transient receptor potential vanilloid (TRPV) calcium channels [21]. The intestine, kidney and placenta are the three major organs implicated in calcium transport by calcium absorption from the diet, renal reabsorption and calcium transport from the maternal to fetal circulation, respectively [5]. In particular, maintenance of the uterine calcium balance is of crucial importance for many physiological functions, including smooth muscle contraction and embryo implantation. To obtain the calcium ion levels required for pregnancy and labor, the body uses numerous calcium transport genes, including CaBPs, PMCA and TRPVs [10,13]. These genes are regulated by various factors, i.e., vitamin D, dietary calcium ion and steroid hormones, and many reports have shown the expression of TRPV6 gene during the estrous cycle and pregnancy [3,6,[28][29][30].The actions of sex steroid hormones, i.e., estrogen (E2)...

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Section: Discussionmentioning

confidence: 88%

mentioning

confidence: 99%

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Duodenal and Renal Transient Receptor Potential Vanilloid 6 Is Regulated by Sex Steroid Hormones, Estrogen and Progesterone, in Immature Rats

Jung

Kim

Yang

et al. 2011

The Journal of Veterinary Medical Science

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“…The regulation of the CaBP-9k gene in an E2-dependent manner has been demonstrated during the estorus cycle in the uterus of ovariectomized rats [8,11]. Because the CaBP-9k gene has been shown to be sensitive to estrogen, we recently hypothesized that the expression of CaBP-9k mRNA and protein could be used as biomarkers to detect estrogenic chemicals in the uteri of rats [19][20][21].…”

Section: Discussionmentioning

confidence: 99%

“…This differential regulation of CaBP-9k is not only limited just to a tissue-specific manner but also extends to a species-specific manner. In the rat uterus, the expression level of CaBP-9k gene is suppressed at diestrus, and increases at proestrus in response to the plasma estrogen levels [8,11]. In ovariectomized rats, estrogen (E2) caused the rapid accumulation of CaBP-9k transcripts in the uterus [9], while progesterone (P4) inhibited the E2-i n d u c e d C a B P -9 k g e n e i n E 2 p r i m e d ovariectomized rat [8].…”

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Differential Transcriptional and Translational Regulations of Calbindm-D9k by Steroid Hormones and Their Receptors in the Uterus of Immature Mice

Choi²,

Hong

et al. 2004

Journal of Reproduction and Development

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Abstract. Calbindin-D9k (CaBP-9k) is a cytosolic calcium binding protein mainly expressed in the duodenum, placenta and uterus, and intestinal CaBP-9k is regulated by 1, 25-dyhydroxyvitamin D3. However, despite the presence of vitamin D receptors, uterine CaBP-9k is not under the control of vitamin D, but seems to be regulated by sex steroids. This steroids-dependent regulation of CaBP-9k is not only limited to a tissue-specific manner but also extends to a species-specific manner. In this study, we examined the regulation of CaBP-9k gene at the transcriptional and translational levels, and also localized CaBP-9k protein in the uterus of immature mice. Treatment with progesterone (P4) resulted in the induction of CaBP-9k mRNA, and a co-treatment with estrogen (E2) plus P4 evoked a synergic effect on its mRNA level in this tissue. Interestingly, the translation of CaBP-9k protein was enhanced by E2, while no difference was observed at the transcriptional level. Not only P4 but also E2 itself induced an increase of CaBP-9k protein, and co-treatment with E2 and P4 showed a similar effect on its protein level in the uterus of immature mice. The CaBP-9k protein was localized in the glandular epithelium of stroma in the uterus of immature mice at diestrus, indicating that the expression of CaBP-9k protein is differentially regulated by sex steroids. A potential mechanism of synergic effect of P4 and E2 may be E2 action in the increase of progesterone receptor (PR), with upregulated PR increasing P4-induced CaBP-9k expression. This complicated relationship between CaBP-9k and steroid receptors suggests that P4 regulates CaBP-9k gene in the uterus of immature mice, in addition, E2 also can affect the expression of CaBP-9k through the regulation of PR. The expression levels of ERα and PR were further examined in this tissue. E2 stimulated the expression levels of ERα and PR mRNAs and P4 inhibited the expression of these transcripts at an early time point (12 h) and increased them at 24 and 48 h, while co-treatments with both steroids increased transcripts of ERα and PR at 24 h. In conclusion, P4 and PR may be dominant factors in the regulation of CaBP-9k. Also, E2 and ERα can influence the expression of the CaBP-9k gene via an indirect pathway in the uterus of immature mice.

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Predicting developmental toxicity through toxicogenomics

Daston

Naciff

2010

Birth Defects Research Pt C

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Global analysis of gene expression in target cells or tissues in response to a toxicant holds significant promise for predictive toxicology. Toxicants elicit a characteristic pattern of gene expression that is dependent on mechanism of action. These mechanism-specific transcript profiles can be used as the basis for predictive toxicology. Potential applications include prioritizing chemicals for testing and customizing testing approaches based on the chemical. Results that are useful in this predictive context can be obtained from animal or in vitro models. Gene expression analysis can also be used to elucidate the shape of the dose-response curve at exposure levels below the no observed adverse effect level, an important need in risk assessment. In this review, we will illustrate each of these points using our research on estrogen and an estrogenic mode of action as a model for how to use gene expression data in a predictive way. Although gene expression in response to estrogens is tissue, life stage, and sex specific, it is feasible to identify transcript profiles that are diagnostic of this mode of action.

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Calbindin-D9k mRNA is tightly regulated during the estrous cycle in the rat uterus

Cited by 56 publications

References 17 publications

Duodenal and Renal Transient Receptor Potential Vanilloid 6 Is Regulated by Sex Steroid Hormones, Estrogen and Progesterone, in Immature Rats

Duodenal and Renal Transient Receptor Potential Vanilloid 6 Is Regulated by Sex Steroid Hormones, Estrogen and Progesterone, in Immature Rats

Differential Transcriptional and Translational Regulations of Calbindm-D9k by Steroid Hormones and Their Receptors in the Uterus of Immature Mice

Predicting developmental toxicity through toxicogenomics

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