Calbindin-D9k gene expression during the perinatal period in the rat: correlation to estrogen receptor expression in uterus

Krisinger, John; Dann, Jamie L.; Applegarth, Oliver; Currie, William D.; Jeung, Eui‐Bae; Staun, Michael; Leung, Peter C.K.

doi:10.1016/0303-7207(93)90211-2

Cited by 29 publications

(15 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the rat, uterine expression of calbindin-D 9k is dependent on critical levels of estradiol-17␤ [41,47] and estrogen receptor [38,40], and it is also tightly regulated during the estrous cycle [48]. The present study is the first to demonstrate that, in the mouse, calbindin-D 9k mRNA level likewise changes during the estrous cycle, indicating hormonal regulation.…”

Section: Discussionmentioning

confidence: 48%

See 1 more Smart Citation

Complex Regulation of Calcium-Binding Protein D9k (Calbindin-D9k) in the Mouse Uterus During Early Pregnancy and at the Site of Embryo Implantation1

Nie

Wang

et al. 2000

Biology of Reproduction

View full text Add to dashboard Cite

Establishment of receptive endometrium is essential for implantation. Our aim was to identify and characterize genes uniquely regulated at the sites of implantation in mouse uterus by RNA differential display polymerase chain reaction (DDPCR). One of the gene fragments identified was 86% homologous to rat calcium-binding protein D9k (calbindin-D(9k)); the mouse counterpart had not then been cloned, but subsequently an mRNA sequence of mouse calbindin-D(9k) became available in GenBank (accession number: AF028071). This sequence is 99% homologous to the DDPCR-derived gene tag but has a shorter 3' end. Reverse transcription-polymerase chain reaction (RT-PCR) was performed using the sequence of 3' end of the DDPCR product and the 5' end of AF028071, and a full cDNA was obtained. This gene was primarily up-regulated by progesterone, but not by estrogen. It was further increased by the combination of the two steroids. Expression of calbindin-D(9k) was overall increased in the uterus during early pregnancy, but the level was significantly lower in implantation compared to interimplantation sites on Days 4.5 and 5.5 of pregnancy, becoming barely detectable in both sites after Day 6.5. In situ hybridization localized this mRNA predominantly in the luminal epithelium of the pregnant uterus. The complex regulation of calbindin-D(9k) in mouse uterus suggests an important role for this protein during pregnancy.

show abstract

Section: Discussionmentioning

confidence: 48%

“…The importance of calbindins in reproductive function has been emphasized from studies involving vitamin D [30][31][32][33][34]. The role of vitamin D in fertility was mostly through its association with the calbindins that were expressed in the ovary, oviducts, testis, and other reproductive tissues [35][36][37][38].…”

Section: Discussionmentioning

confidence: 99%

Complex Regulation of Calcium-Binding Protein D9k (Calbindin-D9k) in the Mouse Uterus During Early Pregnancy and at the Site of Embryo Implantation1

Nie

Wang

et al. 2000

Biology of Reproduction

View full text Add to dashboard Cite

show abstract

“…In rat uterus it has been previously found that CaBP-d9k expression is under strict estrogen regulation (Dupret et al 1992, Krisinger et al 1993, L'Horset et al 1993. However, high expression of CaBP-d9k in porcine uterus during the luteal phase correlates to the regulation of the progesterone gene in that species, due to the lack of a functional ERE.…”

Section: Discussionmentioning

confidence: 94%

“…Previous studies have identified that CaBP-d9k is highly expressed in the mouse and rat uterus during early pregnancy (Darwish et al 1991, Krisinger et al 1992, Krisinger et al 1993, L'Horset et al 1993, Tatsumi et al 1999, Nie et al 2000. In the mouse it is differentially expressed between implantation and inter-implantation sites at the time of initial embryo attachment (Nie et al 2000).…”

Section: Introductionmentioning

confidence: 99%

Endometrial expression of calbindin (CaBP)-d28k but not CaBP-d9k in primates implies evolutionary changes and functional redundancy of calbindins at implantation

et al. 2004

View full text Add to dashboard Cite

The endometrium is hostile to embryo implantation except during the 'window of receptivity'. A change in endometrial gene expression is required for the development of receptivity. Calbindin-d9k (CaBP-d9k) and calbindin-d28k (CaBP-d28k) are proteins possessing EF-hand motifs which have high affinity for Ca 21 ions. Previously, it has been demonstrated that, in mouse endometrium, the expression of both calbindins is highly regulated during implantation and that both proteins play critical but functionally redundant roles at implantation. This study was the first to determine the expression of these two calbindins in the human and rhesus monkey endometrium. Initial RT-PCR analysis demonstrated that CaBP-d28k but not CaBP-d9k mRNA expression is detectable in the endometrium of both species. Western blot analysis confirmed the presence of immunoreactive CaBP-d28k protein in the primate endometrium. Furthermore, the endometrial expression pattern of CaBP-d28k mRNA and protein was examined by Northern blot analysis and immunohistochemistry respectively in both species across the menstrual cycle and during early pregnancy. Semi-quantitative statistical analysis of the immunohistochemistry results revealed that, in the human, CaBP-d28k protein expression was maximal in luminal and glandular epithelium during the mid-secretory phase, coinciding with the time when the endometrium is receptive to embryo implantation. Expression in rhesus monkey showed a similar trend. These results suggest that, in the primate endometrium, only CaBP-d28k is expressed and that the specific regulation of this calbindin is potentially important for the establishment of uterine receptivity.

show abstract

“…The E2 level was highest on day 21 of pregnancy (152 pg/ml) and decreased gradually during lactation starting at L2. This E2 decrease during the lactation period was significantly different when compared to the level on day 21 of pregnancy [27]. Thus, CaBP-9k mRNA expression was analyzed to determine the effect of BPA and E2 on the maternal uterus on day 5 post-delivery.…”

Section: Discussionmentioning

confidence: 99%

Maternal Exposure to Bisphenol A during Late Pregnancy Resulted in an Increase of Calbindin-D9k mRNA and Protein in Maternal and Postnatal Rat Uteri

Hong

Choi²,

Jeung

2005

Journal of Reproduction and Development

Self Cite

View full text Add to dashboard Cite

Abstract. It has been reported that Calbindin-D9k (CaBP-9k) is rapidly and strongly induced by environmental estrogenic compounds, possibly through estrogen receptors (ERα) in the uterus of mammals. CaBP-9k can be evaluated as an early gene marker for assaying estrogenic effects of putative environmental chemicals in the rat uterus. This study was undertaken to investigate CaBP-9k mRNA and protein expression in the postnatal rat uterus following maternal exposure to 17β-estradiol (E2) and bisphenol A (BPA) during the neonatal period. Treatment with a high dose of BPA (600 mg/kg body weight (BW) per day) resulted in a 3-fold increase in CaBP-9k mRNA expression for 3 days, while a single dose of E2 (40 µg/kg BW per day) induced 2-fold increase of this gene in the maternal uterus. In an agreement with maternal CaBP-9k mRNA, postnatal CaBP-9k mRNA in the uterus increased 4-fold when treated with BPA (600 mg/kg BW per day). In addition, treatment with increasing concentrations of BPA resulted in significant increases in CaBP-9k protein in the maternal rat uterus. It is of interest that increasing doses of BPA induced a significant ERα mRNA increase in the postnatal uterus. Furthermore, immunohistochemistry revealed that treatment with BPA induced CaBP-9k protein in the maternal uterus. We demonstrated that maternal exposure to BPA during late pregnancy induced CaBP-9k mRNA and protein in maternal and postnatal rat uteri. These results suggest that rapid absorption and distribution of environmental estrogenic compounds occurs in maternal and neonatal rat uteri and these chemicals can easily pass though the placenta during pregnancy to affect postnatal reproductive functions. Key words: Bisphenol A, Calbindin-D9k, Placental transfer, Postnatal (J. Reprod. Dev. 51: [499][500][501][502][503][504][505][506][507][508] 2005) albindin-D9k (CaBP-9k) and calbindin-D28k belong to a group of cytosolic calcium binding proteins [1]. CaBP-9k is a 9kDa cytoplasmic protein that binds to calcium with high affinity and is expressed predominantly in the mammalian uterus, intestine, and placenta [1,2]. The human CaBP-9k gene, which spans approximately 5.5 kb on the X-chromosome, was cloned and sequenced. It was reported to consist of 3 exons and to carry 4 A l u r ep e a t s [ 3 ] . H u m a n C aB P -9 k i s m o s t homologous to the porcine and bovine homologs (88.6%), followed by rat (78.5%) and murine CaBP9k (75.9%) [4]. In the rat uterus, it has been demonstrated that the CaBP-9k gene is highly regulated by estrogen (E2). CaBP-9k mRNA

show abstract

Calbindin-D9k gene expression during the perinatal period in the rat: correlation to estrogen receptor expression in uterus

Cited by 29 publications

References 24 publications

Complex Regulation of Calcium-Binding Protein D9k (Calbindin-D9k) in the Mouse Uterus During Early Pregnancy and at the Site of Embryo Implantation1

Complex Regulation of Calcium-Binding Protein D9k (Calbindin-D9k) in the Mouse Uterus During Early Pregnancy and at the Site of Embryo Implantation1

Endometrial expression of calbindin (CaBP)-d28k but not CaBP-d9k in primates implies evolutionary changes and functional redundancy of calbindins at implantation

Maternal Exposure to Bisphenol A during Late Pregnancy Resulted in an Increase of Calbindin-D9k mRNA and Protein in Maternal and Postnatal Rat Uteri

Contact Info

Product

Resources

About