Calbindin D-28k and parvalbumin immunohistochemistry in developing rat retina

Uesugi, Ruriko; Yamada, Mitsunori; Mizuguchi, Masashi; Baimbridge, K.G.; Kim, Seung Up

doi:10.1016/0014-4835(92)90127-e

Cited by 47 publications

(47 citation statements)

References 21 publications

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“…In other reports, PV has been detected in amacrine and ganglion cells, and not in horizontal cells of rat retina [3,6,7,19,20,23]. In addition, PV appears in ganglion cells in the middle zone of the neuroblastic layer in the late embryonic period [23]. In the present study, however, we found no PV-immunoreactive ganglion cells from PO to P5.…”

Section: Discussioncontrasting

confidence: 73%

“…It has been reported that both PV and CaBP immunoreactivity are specific to horizontal cells of different mammalian species, including rat [18]. In other reports, PV has been detected in amacrine and ganglion cells, and not in horizontal cells of rat retina [3,6,7,19,20,23]. In addition, PV appears in ganglion cells in the middle zone of the neuroblastic layer in the late embryonic period [23].…”

Section: Discussionmentioning

confidence: 91%

“…Two types of amacrine cells are present in the retina: stratified and diffuse types, and it has been reported that PV is present in diffuse-type amacrine cells in adult rat retina [23]. Therefore, it seems that PV occurs in both types of amacrine cells in the early postnatal days when they are immature, and that it localizes in diffusetype amacrine cells when they mature postnatally.…”

Section: Discussionmentioning

confidence: 96%

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Distribution of .GAMMA.-Aminobutyric Acid (GABA) and the Calcium-binding Protein Parvalbumin in Rat Retina During Development.

Oguni

Setogawa

Shinohara

et al. 1997

Acta Histochem. Cytochem

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Section: Discussioncontrasting

confidence: 73%

Section: Discussionmentioning

confidence: 91%

Section: Discussionmentioning

confidence: 96%

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Distribution of .GAMMA.-Aminobutyric Acid (GABA) and the Calcium-binding Protein Parvalbumin in Rat Retina During Development.

Oguni

Setogawa

Shinohara

et al. 1997

Acta Histochem. Cytochem

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“…The localization of Y1 immunoreactivity to horizontal cells and processes was confirmed in the double-label immunohistochemical experiments using CaBP, a well-established marker for horizontal cells (Röhrenbeck et al, 1987;Uesugi et al, 1992). Nearly all (96%) CaBP-immunoreactive cells contained Y1 immunoreactivity, suggesting that all horizontal cells express Y1 receptor in the rat retina.…”

Section: Horizontal Cells Express Y1mentioning

confidence: 58%

“…2A). Double-labeling experiments using antibodies to CaBP, which label horizontal cells and their processes in the OPL (Röhrenbeck et al, 1987;Uesugi et al, 1992), demonstrated colocalization of Y1 and CaBP in horizontal cell bodies and processes (Fig. 2B).…”

Section: Y1 Immunoreactivity Is Localized To Horizontal Cellsmentioning

confidence: 95%

Localization of neuropeptide Y1 receptor immunoreactivity in the rat retina and the synaptic connectivity of Y1 immunoreactive cells

D'Angelo¹,

Oh²,

Chun³

et al. 2002

J of Comparative Neurology

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Neuropeptide Y (NPY), an inhibitory neuropeptide expressed by a moderately dense population of wide-field amacrine cells in the rat retina, acts through multiple (Y1-y6) G-protein-coupled receptors. This study determined the cellular localization of Y1 receptors and the synaptic connectivity of Y1 processes in the inner plexiform layer (IPL) of the rat retina. Specific Y1 immunoreactivity was localized to horizontal cell bodies in the distal inner nuclear layer and their processes in the outer plexiform layer. Immunoreactivity was also prominent in cell processes located in strata 2 and 4, and puncta in strata 4 and 5 of the IPL. Double-label immunohistochemical experiments with calbindin, a horizontal cell marker, confirmed Y1 immunostaining in all horizontal cells. Double-label immunohistochemical experiments, using antibodies to choline acetyltransferase and vesicular acetylcholine transporter to label cholinergic amacrine cell processes, demonstrated that Y1 immunoreactivity in strata 2 and 4 of the IPL was localized to cholinergic amacrine cell processes. Electron microscopic studies of the inner retina showed that Y1-immunostained amacrine cell processes and puncta received synaptic inputs from unlabeled amacrine cell processes (65.2%) and bipolar cell axon terminals (34.8%). Y1-immunoreactive amacrine cell processes most frequently formed synaptic outputs onto unlabeled amacrine cell processes (34.0%) and ganglion cell dendrites (54.1%). NPY immunoreactivity in the rat retina is distributed primarily to strata 1 and 5 of the IPL, and the present findings, thus, suggest that NPY acts in a paracrine manner on Y1 receptors to influence both horizontal and amacrine cells. Indexing termsamacrine cells; choline acetyltransferase; calcium binding protein; horizontal cells; neuropeptides Neuropeptide Y (NPY) has multiple physiologic actions in both the central and peripheral nervous system, including effects on blood flow, memory retention, food intake, epilepsy, and pain (Lundberg et al., 1996;Munglani et al., 1996;Balasubramaniam, 1997;Blomqvist and Herzog, 1997;Thorsell et al., 2000;Furtinger et al., 2001;Naveilhan et al., 2001). These NIH-PA Author ManuscriptNIH-PA Author Manuscript NIH-PA Author Manuscript effects are mediated by means of at least five G-protein-coupled receptors, designated as Y1, Y2, Y4, Y5, and y6, which are coupled to pertussis toxin-sensitive inhibitory Gproteins (Lundberg et al., 1996;Munglani et al., 1996;Balasubramaniam, 1997;Blomqvist and Herzog, 1997;Michel et al., 1998). Y-receptors have been localized to specific regions of the central and peripheral nervous system by receptor binding autoradiography, immunohistochemistry, and in situ hybridization (Zhang et al., 1994;Bao et al., 1997;Jacques et al., 1997;Dumont et al., 1998;Hökfelt et al., 1998;Gackenheimer et al., 2001). Y1 receptor is the most studied Y-receptor and it plays key roles in many of the central and peripheral effects of NPY (Lundberg et al., 1996;Munglani et al., 1996;Balasubramaniam, 1997;Blomqvist and H...

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Specific expression of the LIM/Homeodomain protein Lim-1 in horizontal cells during retinogenesis

2000

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The LIM/homeodomain transcription factor Lim-1 has been shown to play an essential role in early embryonic patterning during vertebrate development. Here we report the spatial and temporal expression patterns of Lim-1 during retinal development as detected by immunohistochemistry using a specific anti-Lim-1 antibody. By double-immunostaining, we have demonstrated for the first time that Lim-1 is exclusively expressed within the horizontal cell type in the adult retina. In the developing mouse retina, Lim-1 commences its expression in migratory horizontal cell precursors streaming toward the future horizontal cell layer in the ventricular zone. Moreover, its expression during retinogenesis is spatially and temporally coincident with that of the calcium-binding protein calbindin D-28k in horizontal cells. These data together suggest a possible role for Lim-1 in terminal differentiation and maintenance of horizontal cells, and that Lim-1 can serve as a specific molecular marker for the study of horizontal cell specification.

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Calbindin D-28k and parvalbumin immunohistochemistry in developing rat retina

Cited by 47 publications

References 21 publications

Distribution of .GAMMA.-Aminobutyric Acid (GABA) and the Calcium-binding Protein Parvalbumin in Rat Retina During Development.

Distribution of .GAMMA.-Aminobutyric Acid (GABA) and the Calcium-binding Protein Parvalbumin in Rat Retina During Development.

Localization of neuropeptide Y1 receptor immunoreactivity in the rat retina and the synaptic connectivity of Y1 immunoreactive cells

Specific expression of the LIM/Homeodomain protein Lim-1 in horizontal cells during retinogenesis

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