Cajaninstilbene acid analogues as novel quorum sensing and biofilm inhibitors of Pseudomonas aeruginosa

Huang, Zhi-Xing; Yu, Jiahui; Xu, Xingjun; Xu, Xiaofang; Zeng, Ting; Lin, Jing; Chen, Weimin

doi:10.1016/j.micpath.2020.104414

Cited by 7 publications

(3 citation statements)

References 62 publications

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“…Specifically, CSA exhibits a neuroprotective function in vivo by activating the AMPK/Nrf2 pathway to reduce mitochondrial dysfunction [ 5 ]. CSA has also shown antibacterial activity against gram-positive bacteria including vancomycin-resistant Enterococcus , Staphylococcus aureus , and Bacillus subtilis , and antibiofilm activity against gram-negative bacteria including Pseudomonas aeruginosa [ 6 , 7 , 8 ]. In addition, CSA has demonstrated anti-inflammatory activity both in vivo and in vitro by inhibiting NF-κB and MAPK pathways [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

Production and Secretion of Isowighteone in Hairy Root Cultures of Pigeon Pea (Cajanus cajan) Co-Treated with Multiple Elicitors

Gajurel

Ñopo-Olazabal

Hendrix

et al. 2022

Plants

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Cajaninstilbene acid (CSA) is a prenylated stilbenoid derivative that exhibits neuroprotective, antibacterial, and anti-inflammatory properties. To establish a bioproduction system for this bioactive compound, hairy root cultures of pigeon pea (Cajanus cajan (L.) Millsp.) were developed via Agrobacterium rhizogenes-mediated transformation. The cultures were co-treated with methyl jasmonate, cyclodextrin, hydrogen peroxide, and magnesium chloride to enhance the production of CSA. The amount of CSA that accumulated in the culture medium upon elicitation varied with the period of elicitation. CSA was purified from extracts of the culture medium by semi-preparative HPLC, and its identity was confirmed by tandem mass spectrometry. After 144 h of elicitation in 12-day-old hairy root culture, the total yield of CSA was 8058.618 ± 445.78 μg/g DW, of which approximately 96% was found in the culture medium. The yield of CSA in the elicited hairy root culture was approximately 277-fold higher than in the non-elicited root culture. The difference between the phenotypes of the elicited and non-elicited pigeon pea hairy roots was studied using scanning electron microscopy. The non-elicited hairy roots had uniform surfaces whereas the elicited roots had non-uniform shapes. Pigeon pea hairy roots provide a sustainable platform for producing and studying the biosynthesis of CSA.

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Section: Introductionmentioning

confidence: 99%

Production and Secretion of Isowighteone in Hairy Root Cultures of Pigeon Pea (Cajanus cajan) Co-Treated with Multiple Elicitors

Gajurel

Ñopo-Olazabal

Hendrix

et al. 2022

Plants

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“…Additionally, compound 8c showed suppression on expression of lasB-lacZ and pqsA-lacZ involved in the QS network pathway in P. aeruginosa. Thereby proving compound 8c as a promising lead with inhibition of QS and associated biofilm formation in P. aeruginosa [160,161].…”

Section: Cajaninstilbene Acid Derivativesmentioning

confidence: 94%

Development of Antibiofilm Therapeutics Strategies to Overcome Antimicrobial Drug Resistance

et al. 2022

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A biofilm is a community of stable microorganisms encapsulated in an extracellular matrix produced by themselves. Many types of microorganisms that are found on living hosts or in the environment can form biofilms. These include pathogenic bacteria that can serve as a reservoir for persistent infections, and are culpable for leading to a broad spectrum of chronic illnesses and emergence of antibiotic resistance making them difficult to be treated. The absence of biofilm-targeting antibiotics in the drug discovery pipeline indicates an unmet opportunity for designing new biofilm inhibitors as antimicrobial agents using various strategies and targeting distinct stages of biofilm formation. The strategies available to control biofilm formation include targeting the enzymes and proteins specific to the microorganism and those involved in the adhesion pathways leading to formation of resistant biofilms. This review primarily focuses on the recent strategies and advances responsible for identifying a myriad of antibiofilm agents and their mechanism of biofilm inhibition, including extracellular polymeric substance synthesis inhibitors, adhesion inhibitors, quorum sensing inhibitors, efflux pump inhibitors, and cyclic diguanylate inhibitors. Furthermore, we present the structure–activity relationships (SAR) of these agents, including recently discovered biofilm inhibitors, nature-derived bioactive scaffolds, synthetic small molecules, antimicrobial peptides, bioactive compounds isolated from fungi, non-proteinogenic amino acids and antibiotics. We hope to fuel interest and focus research efforts on the development of agents targeting the uniquely complex, physical and chemical heterogeneous biofilms through a multipronged approach and combinatorial therapeutics for a more effective control and management of biofilms across diseases.

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“…Amorfrutins B and synthesized 4 8a – c ( Figure 15 a) were analogues of the natural products cajaninstilbene acid (CSA). They can inhibit the las system and las system-related virulence factors by competitively binding with the receptor OdDHL; they can also interrupt pqs and show biofilm inhibitory activity [ 109 , 110 ]. It was found that a caffeic acid derivative with phenolic hydroxyl group 49 ( Figure 15 a) played the same role as amorfrutins B.…”

Section: Inhibitors Acting On Multiple Qs Systemsmentioning

confidence: 99%

The Molecular Architecture of Pseudomonas aeruginosa Quorum-Sensing Inhibitors

Shen

Wang

et al. 2022

Marine Drugs

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The survival selection pressure caused by antibiotic-mediated bactericidal and bacteriostatic activity is one of the important inducements for bacteria to develop drug resistance. Bacteria gain drug resistance through spontaneous mutation so as to achieve the goals of survival and reproduction. Quorum sensing (QS) is an intercellular communication system based on cell density that can regulate bacterial virulence and biofilm formation. The secretion of more than 30 virulence factors of P. aeruginosa is controlled by QS, and the formation and diffusion of biofilm is an important mechanism causing the multidrug resistance of P. aeruginosa, which is also closely related to the QS system. There are three main QS systems in P. aeruginosa: las system, rhl system, and pqs system. Quorum-sensing inhibitors (QSIs) can reduce the toxicity of bacteria without affecting the growth and enhance the sensitivity of bacterial biofilms to antibiotic treatment. These characteristics make QSIs a popular topic for research and development in the field of anti-infection. This paper reviews the research progress of the P. aeruginosa quorum-sensing system and QSIs, targeting three QS systems, which will provide help for the future research and development of novel quorum-sensing inhibitors.

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Cajaninstilbene acid analogues as novel quorum sensing and biofilm inhibitors of Pseudomonas aeruginosa

Cited by 7 publications

References 62 publications

Production and Secretion of Isowighteone in Hairy Root Cultures of Pigeon Pea (Cajanus cajan) Co-Treated with Multiple Elicitors

Production and Secretion of Isowighteone in Hairy Root Cultures of Pigeon Pea (Cajanus cajan) Co-Treated with Multiple Elicitors

Development of Antibiofilm Therapeutics Strategies to Overcome Antimicrobial Drug Resistance

The Molecular Architecture of Pseudomonas aeruginosa Quorum-Sensing Inhibitors

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