Caiman latirostris (broad-snouted caiman) as a sentinel organism for genotoxic monitoring: Basal values determination of micronucleus and comet assay

Poletta, Gisela Laura; Larriera, Alejandro; Kleinsorge, E. C.; Mudry, Marta Dolores

doi:10.1016/j.mrgentox.2007.12.001

Cited by 66 publications

(31 citation statements)

References 42 publications

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“…As for crocodiles, caimans were used to validate the comet assay and later on to test several agricultural chemicals on the extent of DNA damage in blood cells. The comet assay was first validated in broad-snouted caiman (Caiman latirostris) marking this species as a suitable sentinel organism for the genotoxic assessment of environmental pollutants [285].…”

Section: Reptilesmentioning

confidence: 99%

The comet assay in animal models: From bugs to whales – (Part 2 Vertebrates)

Gajski

Žegura

Ladeira

et al. 2019

Mutation Research/Reviews in Mutation Research

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The comet assay has become one of the methods of choice for the evaluation and measurement of DNA damage. It is sensitive, quick to perform and relatively affordable for the evaluation of DNA damage and repair at the level of individual cells. The comet assay can be applied to virtually any cell type derived from different organs and tissues. Even though the comet assay is predominantly used on human cells, the application of the assay for the evaluation of DNA damage in yeast, plant and animal cells is also quite high, especially in terms of biomonitoring. The present extensive overview on the usage of the comet assay in animal models will cover both terrestrial and water environments. The first part of the review was focused on studies describing the comet assay applied in invertebrates. The second part of the review, (Part 2) will discuss the application of the comet assay in vertebrates covering cyclostomata, fishes, amphibians, reptiles, birds and mammals, in addition to chordates that are regarded as a transitional form towards vertebrates. Besides numerous vertebrate species, the assay is also performed on a range of cells, which includes blood, liver, kidney, brain, gill, bone marrow and sperm cells. These cells are readily used for the evaluation of a wide spectrum of genotoxic agents both in vitro and in vivo. Moreover, the use of vertebrate models and their role in environmental biomonitoring will also be discussed as well as the comparison of the use of the comet assay in vertebrate and human models in line with ethical principles. Although the comet assay in vertebrates is most commonly used in laboratory animals such as mice, rats and lately zebrafish, this paper will only briefly review its use regarding laboratory animal models and rather give special emphasis to the increasing usage of the assay in domestic and wildlife animals as well as in various ecotoxicological studies.

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Section: Reptilesmentioning

confidence: 99%

The comet assay in animal models: From bugs to whales – (Part 2 Vertebrates)

Gajski

Žegura

Ladeira

et al. 2019

Mutation Research/Reviews in Mutation Research

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“…The micronucleus assay is a cytogenetic method that is used as a biomarker assay in contaminated areas (Torres-Bugarín et al, 2007;Polleta et al, 2008;Galindo and Moreira, 2009). It consists of determining the frequency of EMN in blood samples using optical microscopy (Schmid, 1975(Schmid, , 1976.…”

Section: The Basis Of the Emn Assaymentioning

confidence: 99%

Seabird Embryos as Biomonitors of Micronucleogenic Genotoxic Agents: Potential Application for the Coasts of Mexico

Ceyca

Torres-Bugarı́n

Castillo-Guerrero

et al. 2014

Avian Biology Research

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Genotoxic, teratogenic, and cytotoxic chemical agents are often products of anthropogenic activities, such as agriculture and industry, and many eventually settle in marine and coastal ecosystems. Therefore, there is a need to implement monitoring programs that provide early warnings of the risks and problems associated with contamination to contribute to the conservation of coastal ecosystems and human health. This paper describes the fundamental characteristics of a micronucleus assay that uses seabird embryo blood and highlights its ability to detect the presence of micronucleogenic genotoxic agents along the coasts of Mexico. This paper also demonstrates the potential of this assay to assess possible seasonal and spatial effects of these agents on wildlife, which has direct implications for human health.

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“…Concentrations from multiple pesticides also may act additively, resulting in a total concentration that might be biologically relevant, even within less contaminated habitats . Detrimental effects in high‐trophic‐level crocodilians are all the more troubling because they may signal consequences for the entire ecosystem .…”

Section: Introductionmentioning

confidence: 99%

“…The spectacled caiman ( Caiman crocodilus ) is a predatory reptile that lives in freshwater habitats in the Americas. Within the North Atlantic region of Costa Rica, adult spectacled caiman are relatively abundant, territorial, and tend to have high site fidelity . Although spectacled caiman are generalist predators, most of the adult diet consists of fish .…”

Section: Introductionmentioning

confidence: 99%

Pesticides in blood from spectacled caiman (Caiman crocodilus) downstream of banana plantations in Costa Rica

Grant¹,

Woudneh

Ross

2013

Enviro Toxic and Chemistry

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Spectacled caiman (Caiman crocodilus) are fish-eating crocodilians that inhabit freshwater habitat in tropical regions of the Americas. To assess the exposure of caiman to pesticides from banana plantations, the authors collected whole blood samples (30 mL) from 14 adult caiman that were captured in the North Atlantic region of Costa Rica. Blood samples were analyzed for 70 legacy- and current-use pesticides and breakdown products using newly developed ultra-trace, high-resolution mass spectrometry (HRMS). Caiman accumulated pesticides ranked by concentration as dieldrin > permethrin > mirex > 4,4'-DDE > alpha-endosulfan > heptachlor epoxide > oxychlordane > heptachlor > cypermethrin. Caiman within the high-intensity banana crop watershed of Rio Suerte had higher pesticide burdens relative to other more remote locations (F = 12.79; p = 0.00). Pesticide concentration decreased with distance from upstream banana plantations in this river system (F = 20.76; p = 0.00). Caiman body condition was negatively correlated with total pesticide concentrations (F = 6.23; p = 0.02) and with proximity to banana plantations (F = 5.05; p = 0.04). This suggests that either pesticides elicited toxic effects in caiman, resulting in diminished overall health, or that the quantity or quality of their prey was reduced by pesticides downstream of plantation waterways. The authors' results indicate that pesticide use in banana plantations is impacting a high trophic level species inhabiting one of the most important wilderness areas in Costa Rica (Tortuguero National Park).

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Caiman latirostris (broad-snouted caiman) as a sentinel organism for genotoxic monitoring: Basal values determination of micronucleus and comet assay

Cited by 66 publications

References 42 publications

The comet assay in animal models: From bugs to whales – (Part 2 Vertebrates)

The comet assay in animal models: From bugs to whales – (Part 2 Vertebrates)

Seabird Embryos as Biomonitors of Micronucleogenic Genotoxic Agents: Potential Application for the Coasts of Mexico

Pesticides in blood from spectacled caiman (Caiman crocodilus) downstream of banana plantations in Costa Rica

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