Caged and Wild Fish: Induction of Hepatic Cytochrome P-450 (Cyp1a1) as an Environmental Biomonitor

Haasch, Mary L.; Prince, Ruth J.; Wejksnora, Peter J.; Cooper, Keith; Lech, John J.

doi:10.1897/1552-8618(1993)12[885:cawfio]2.0.co;2

Cited by 20 publications

(24 citation statements)

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“…Total cytochrome P450 content (P450+P420) in Coryphaenoides guentheri and Lepidion lepidion were in the higher range (0.9 to 2.0 m01 mg-' protein) reported for teleosts. The specific activity of NADPH-cytochrome c (P450) reductase, the microsomal flavoprotein that transfers electrons from NADPH to cytochrome P450, was also similar to that reported for other species (Mathieu et al 1991, Haasch et al 1993, Sleiderink et al 1995. Therefore, a high monooxygenase capacity of deep-sea fish hepatic tissue could be anticipated.…”

Section: Discussionsupporting

confidence: 77%

Xenobiotic metabolising enzymes and antioxidant defences in deep-sea fish:relationship with contaminant body burden

Porte¹,

Escartı́n²,

García³

et al. 2000

Mar. Ecol. Prog. Ser.

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Xenobiotic metabolising enzymes, namely the cytochrome P450 monooxygenase system and glutathione S-transferase, as well as antioxidant enzymes were determined in the liver of 3 deepsea fish species (tepidion lepidion, Bathypterois mediterraneusand Coryphaenoides guenthen). These organisms were collected from 3 stations along the NW Mediterranean, at depths ranging from 1500 to 1800 m. The enzymatlc activities detected in deep-sea fish indicated the ability of these organisms to cope with pollutants and oxidative stress. Differences among the studied species may be related to habitat and diet, viz. the highest monooxygenase and antioxidant enzyme activities were observed in L. lepidion, a typical middle slope species, whereas the lowest activities were recorded for B. mediterraneus, an organism adapted to live at greater depths. Tissue concentration of persistent organochlorinated compounds, like polychlorinated biphenyls, DDTs and hexachlorobenzene, were in the lower range of those reported for coastal fish, and significant differences among sampling stations were not observed. Overall, the obtained results contribute to the knowledge of the NW Mediterranean deepsea basin and reflect the chronic contamination of the area in terms of organochlorinated compounds, rather than the influence of coastal discharges.

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Section: Discussionsupporting

confidence: 77%

Xenobiotic metabolising enzymes and antioxidant defences in deep-sea fish:relationship with contaminant body burden

Porte¹,

Escartı́n²,

García³

et al. 2000

Mar. Ecol. Prog. Ser.

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“…The hepatic microsomal cytochrome P450 content of Mullus barbatus was in the range reported for other teleosts (0.4 to 0.8 nmol mg -1 protein), and was not inducted by exposure to pollutants as reported by other authors for other species (Ueng et al 1992, Haasch et al 1993, probably due to the fact that our assay measures both constitutive and inducible isoforms of cytochrome P450.…”

Section: Biomarkerssupporting

confidence: 79%

“…* Significant difference from reference site at Stn 5; +: significant difference from reference site at Stn 10 laboratory studies in this respect. Haasch et al (1993) found that the largemouth bass Micropterus salmoides, exposed to 0.28 mg l -1 β-naphthoflavone, a known CYP1A inducer, showed a 6.4-fold increase in EROD activity 1 d after exposure, and a 25-fold increase in immunoreactive CYP1A. On the other hand, the opposite trend was observed for the rainbow trout Oncorhynchus mikiss treated with an i.p.…”

Section: Biomarkersmentioning

confidence: 92%

Assessment of coastal pollution by combined determination of chemical and biochemical markers in Mullus barbatus

Porte¹,

Escartı́n²,

Parra³

et al. 2002

Mar. Ecol. Prog. Ser.

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Muscle concentrations of organochlorinated compounds as well as biliary levels of polycyclic aromatic hydrocarbon (PAH) metabolites were determined in red mullet Mullus barbatus as a sentinel species for assessing the pollution along the western Mediterranean coast. A battery of biochemical markers -biotransformation and antioxidant enzymes -were also measured in liver subcellular fractions to assess exposure to pollutants. Among them, 7-ethoxyresorufin O-deethylase (EROD) activity, as well as 7-penthoxyresorufin O-deethylase (PROD) activity and cytochrome P450 1A (CYP1A) content evidenced strong differences among sampling sites, and a good correlation with the amount of PCBs bioaccumulated by fish. No clear pollution-related response was observed for cytosolic glutathione S-transferase, whereas uridine-diphosphate UDP-glucuronyltransferase (UDPGT) was elevated in fish from polluted sites. Antioxidant enzymes -superoxide dismutase (SOD), catalase and glutathione peroxidase (GPX) -were used to assess oxidative stress. Among them, catalase activity was well related to PCB body burden.

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“…Caged fish had already been used as a monitoring tool and this method has proved reliable (Haasch et al, 1993;Jedamski-Grymlas et al, 1994;Lindstro¨m-Seppa¨and Oikari, 1989). However, caged fish may suffer from density stress, which could increase cortisol levels (Bucheli and Fent, 1995).…”

Section: On-site Experimental Tanksmentioning

confidence: 99%

Impact Assessment of a Wastewater Treatment Plant Effluent Using the Fish Biomarker Ethoxyresorufin-O-Deethylase: Field and On-Site Experiments

Kosmala¹,

Migeon²,

Flammarion³

et al. 1998

Ecotoxicology and Environmental Safety

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Caged and Wild Fish: Induction of Hepatic Cytochrome P-450 (Cyp1a1) as an Environmental Biomonitor

Cited by 20 publications

References 0 publications

Xenobiotic metabolising enzymes and antioxidant defences in deep-sea fish:relationship with contaminant body burden

Xenobiotic metabolising enzymes and antioxidant defences in deep-sea fish:relationship with contaminant body burden

Assessment of coastal pollution by combined determination of chemical and biochemical markers in Mullus barbatus

Impact Assessment of a Wastewater Treatment Plant Effluent Using the Fish Biomarker Ethoxyresorufin-O-Deethylase: Field and On-Site Experiments

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