CagA subtyping in Helicobacter pylori isolates from gastric cancer patients in an ethnic Kashmiri population

Qadri, Qurteeba; Afroze, Dil; Rasool, Roohi; Gulzar, Ghulam Mohammad; Naqash, Sameer H.; Siddiqi, Mushtaq A.; Shah, Zafar A.

doi:10.1016/j.micpath.2013.12.004

Cited by 8 publications

(6 citation statements)

References 45 publications

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“…In India and Philippines, both eastern and western patterns have been observed [ 40 , 41 ]. In the current study, various patterns of the EPIYA motifs were observed including AB, BC, and AC, consistent with other studies [ 6 , 31 , 33 ].…”

Section: Resultssupporting

confidence: 92%

“…pylori infection [ 5 ]. Environmental factors, host genetic characteristics, and bacterial pathogenic factors contribute to the final outcome of bacterial pathogenesis [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

“…The prevalence of H. pylori infection is comparatively higher in developing countries such that over 80% of the people in these countries are infected with H. pylori infection [5]. Environmental factors, host genetic characteristics, and bacterial pathogenic factors contribute to the final outcome of bacterial pathogenesis [6].…”

Section: Introductionmentioning

confidence: 99%

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Determination of CagA EPIYA motif in Helicobacter pylori strains isolated from patients with digestive disorder

Khaledi

Bagheri

Validi

et al. 2020

Heliyon

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This study was conducted to identify patterns of cagA EPIYA motifs in H . pylori strains isolated from patients with gastrointestinal diseases in Hospitals of Shahrekord, and investigate the association between these biomarkers and clinical outcomes of gastrointestinal diseases due to H . pylori . In this study, 253 patients with gastrointestinal diseases were studied within 1395–1396. Histopathological investigations and urease test showed that 207 isolates were H . pylori -positive. Then, screening using a molecular technique, PCR, confirmed that 159 isolates had cagA. Finally, the pattern and prevalence of the motifs were determined by PCR and identified a number of motifs were sequenced. Results of this study showed that the pattern of motifs was as follows: ABC (140 isolates) (93/7%), ABCC (6 isolates) (3/77%), ABCCC (4 isolates) (2/5%), AB (7 isolates) (4/4%), AC (1 isolate) (0/6%), and BC (1 isolate) (0/6%). Sequencing results showed the presence of changed EPIYA motif in some isolates. CM motif sequence was also seen in all isolates. In this study, no significant association was seen between the prevalence rate of different patterns and clinical symptoms ( p = 0.71). There is a slight association between the presence of ABC motifs and the type of digestive disorder (p = 0.056). Results indicated that ABC was the most frequently seen pattern however, in such that positive cases of ABC motifs were more common in gastritis. All isolates had kinase phosphorylation region, and the observed pattern in this region was a generally western type (ABC).

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Section: Resultssupporting

confidence: 92%

“…pylori infection [ 5 ]. Environmental factors, host genetic characteristics, and bacterial pathogenic factors contribute to the final outcome of bacterial pathogenesis [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

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Determination of CagA EPIYA motif in Helicobacter pylori strains isolated from patients with digestive disorder

Khaledi

Bagheri

Validi

et al. 2020

Heliyon

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“…Similarly, Sicinschi et al (2010) indicated that two EPIYA-C (34.3 %) and three EPIYA-C (1.5 %) repeats were found in patients with GC and suggested a significant association between the multiple EPIYA-C repeats and more severe lesions. Qadri et al (2014) found that 70 % of CagA-positive GC cases had more than one EPIYA-C repeat. Ajami et al (2013) found EPIYA-ABCC in 36.2 % of the patients with GC, and patients with EPIYA-C had a 2.27-fold increase in GC development versus patients with DU.…”

mentioning

confidence: 97%

Patterns of EPIYA motifs among cagA-positive Helicobacter pylori strains: a case–control study in a Turkish population with Eurasian geographical features

Kocazeybek

Caliskan

Cetin

et al. 2015

Journal of Medical Microbiology

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Geographical variation in the frequency of various gastroduodenal pathologies was shown to be related to the geographical diversity of H. pylori CagA Glu-Pro-Ile-Tyr-Ala (EPIYA) patterns. We examined the EPIYA patterns of H. pylori and the association of EPIYA patterns with gastric cancer (GC) for the first time, to the best of our knowledge, in Turkey. The patient group (PG) contained 60 patients [38 GC and 22 duodenal ulcer (DU) patients]. The control group (CG) was 110 individuals [94 gastritis patients and 16 persons with a normal gastrointestinal system (NGIS)]. Specific primers were used for the detection of cagA including empty-site-positive and EPIYA-A, -B, -C, -D PCR. Bands of EPIYA-A, -B, -C were confirmed by DNA sequencing. One hundred and forty-two (83.5 %) strains [60 in the PG (38 GC, 22 DU), 82 in the CG (72 gastritis, 10 NGIS)] were positive for the cagA gene. EPIYA-C with multiple repeats was detected in 34 (23.9 %) strains, and 22 (64.7 %) were from GC patients. EPIYA-C with one repeat was detected in 89 (62.7 %) strains, and 54 (60.7 %) were from gastritis patients. EPIYT was detected in 10 strains, and EPIYA-D was not detected. The number of EPIYA-C with multiple repeats was significantly higher for the PG than for the CG (P,0.0001). In GC patients, the number of EPIYA-C with multiple repeats was significantly higher than one repeat (P,0.0001). In conclusion, our study showed that multiple EPIYA-C repeats increases the GC risk by 30.6-fold and the DU risk by 8.9-fold versus the CG. This indicates that Western-type H. pylori strains in Turkey have similar EPIYA motifs to those of neighbouring countries and Western populations. INTRODUCTIONHelicobacter pylori is the principal cause of gastritis and gastric or duodenal ulcer (DU) diseases and is involved in the development of gastric cancer (GC) and mucosaassociated lymphoid tissue lymphoma (Atherton, 2006).H. pylori is a class 1 carcinogen identified by the International Agency for Research on Cancer for chronic and persistent infections. It is resistant to antibacterial agents in spite of innate and adaptive immunity (International Agency for Research on Cancer, 1994). Depending on virulence factors such as cytotoxin-associated gene A (CagA), blood group antigen-binding adhesion (BabA2) and vacuolating cytotoxin gene A (VacA), different genotypes cause different pathological and clinical outcomes and geographical regional differences (Atherton et al., 1995;Basso et al., 2008). Recently, the Glu-Pro-Ile-Tyr-Ala (EPIYA)Abbreviations: cagPAI, cag pathogenicity island; CG, control group; CI, confidence interval; DU, duodenal ulcer; EPIYA, Glu-Pro-Ile-Tyr-Ala; CagA, cytotoxin-associated gene A; GC, gastric cancer; NGIS, normal gastrointestinal system; PG, patient group; OR, odds ratio. pattern of CagA was suggested to be involved in the pathogenesis of GC, as this pattern manifests important variations depending on the region (Argent et al., 2004(Argent et al., , 2005.The CagA protein is injected into host epithelial cells with its peptidoglycan b...

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“…Only six studies were included in the meta-analysis (Aziz, Chen, Yang, & Yan, 2014;Lima, Silva-Fernandes, Alves, & Rabenhorst, 2011;López-Vidal, Ponce-de-León, Castillo-Rojas, Barreto-Zúñiga, & Torre-Delgadillo, 2008;Mattar et al, 2010;Qadri et al, 2014;Sanei et al, 2005). The combined frequency of H. pylori was 61.14% (56.8-65.7%) in GC patients.…”

Section: Frequency Of H Pylori In Gc Patientsmentioning

confidence: 99%

Global estimate of gastric cancer in Helicobacter pylori–infected population: A systematic review and meta‐analysis

Pormohammad

Mohtavinejad

Gholizadeh

et al. 2018

Journal Cellular Physiology

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There is information regarding the rates of gastric cancer (GC) in different populations and the important role of Helicobacter pylori in GC development; however, no comprehensive study has yet been performed to investigate the prevalence of GC in H. pylori-infected patients. PubMed, Embase, and Cochrane Library through January 1, 2000 were searched without language restrictions. Quality of included studies was assessed with a critical appraisal checklist recommended by the Joanna Briggs Institute. All of the analyses were conducted using Comprehensive Meta-Analysis Software Version 2.0 and Stata 14.0. Forty-four studies from 17 countries were included. The pooled frequency of GC was 17.4% (95% confidence interval: 16.4-18.5) in H. pylori-infected population. The frequency of GC among H. pylori-infected population varied markedly across countries. The highest rate of GC was observed in H. pylori-infected individuals from Asian countries. The frequency of GC was relatively high in H. pylori-infected population in the world. However, the eradication of H. pylori might be a promising strategy for GC prevention, especially in high-risk populations such as Asian countries.

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CagA subtyping in Helicobacter pylori isolates from gastric cancer patients in an ethnic Kashmiri population

Cited by 8 publications

References 45 publications

Determination of CagA EPIYA motif in Helicobacter pylori strains isolated from patients with digestive disorder

Determination of CagA EPIYA motif in Helicobacter pylori strains isolated from patients with digestive disorder

Patterns of EPIYA motifs among cagA-positive Helicobacter pylori strains: a case–control study in a Turkish population with Eurasian geographical features

Global estimate of gastric cancer in Helicobacter pylori–infected population: A systematic review and meta‐analysis

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