Caffeoylquinic Acid Derivatives of Purple Sweet Potato as Modulators of Mitochondrial Function in Mouse Primary Hepatocytes

Abstract

Owing to their antioxidant properties, caffeoylquinic acid (CQA)-derivatives could potentially improve the impaired metabolism in hepatic cells, however, their effect on mitochondrial function has not been demonstrated yet. Here, we evaluated the impact of three CQA-derivatives extracted from purple sweet potato, namely 5-CQA, 3,4- and 4,5-diCQA, on mitochondrial activity in primary hepatocytes using an extracellular flux analyzer. Notably, an increase of maximal respiration and spare respiratory capacity were observed when 5-CQA and 3,4-diCQA were added to the system indicating the improved mitochondrial function. Moreover, 3,4-diCQA was shown to considerably increase glycolytic reserve which is a measure of cell capability to respond to an energy demand through glycolysis. Conversely, 4,5-diCQA did not modify mitochondrial activity but increased glycolysis at low concentration in primary hepatocytes. All compounds tested improved cellular capacity to oxidize fatty acids. Overall, our results demonstrated the potential of test CQA-derivatives to modify mitochondrial function in hepatic cells. It is especially relevant in case of dysfunctional mitochondria in hepatocytes linked to hepatic steatosis during obesity, diabetes, and metabolic syndrome.

Analysis of phytochemical composition and biological activities of Helichrysum pallasii (Sprengel) ledeb leaves

“…The dominant phenolic compounds of the Helichrysum pallasii extracts were chlorogenic acid isomers which were previously reported as potent antidiabetic agents in vitro (𝛼glucosidase; IC50: 9.1 μg/ml and α-amylase; IC50: 9.2 μg/ml) (Oboh et al, 2015) and in vivo (Jung et al, 2006;Kamalakkannan and Prince, 2006;Ong et al, 2012;Torres et al, 2021).…”

“…The most abundant form of chlorogenic acid in nature is 5-caffeoylquinic acid (Olthof et al, 2001). Chlorogenic acid isomers (3-caffeoylquinic, 4-caffeoylquinic, and 5caffeoylquinic acids) show many biological activities such as antibacterial, antioxidant, anticarcinogenic, hypoglycaemic, hypolipidemic, hepatoprotective, antihistamine antimutagenic, anti-obesity, antidiabetic, antiviral, anti-Alzheimer, neuroprotective (Clifford et al, 2017;Hamed et al, 2020;Matthews et al, 2020;Torres et al, 2021). Rutin is a flavonoid found in many plants, also considered a non-toxic chemical that may be useful in biomedical applications and showing biological activities such as anti-inflammatory, anticarcinogenic, antioxidant, neuroprotective, cytoprotective, vasoprotective, cardioprotective, and hepatoprotective (Ghorbani, 2017;Shahi et al, 2019).…”

Antioxidant and Mitochondria-Targeted Activity of Caffeoylquinic-Acid-Rich Fractions of Wormwood (Artemisia absinthium L.) and Silver Wormwood (Artemisia ludoviciana Nutt.)

“…Torres et al indicated that the caffeoylquinic acids in acetone:water:lactic acid (40:60:1) extract from purple sweet potatoes’ tuberous roots have the potential to improve impaired mitochondrial function in hepatocytes and to increase fatty acid oxidation. They demonstrated that the addition of 5-caffeoylquinic acid or 3,4-dicaffeoylquinic acid increased the maximal respiration and the reserve respiratory capability in primary hepatocytes [ 52 ]. We found that caffeoylquinic-acid-rich fractions from wormwood and silver wormwood herb acetone extracts can modulate mitochondrial function, and that the effect on mitochondria is dose-dependent.…”

3,4,5‐O‐tricaffeoylquinic acid alleviates ionizing radiation‐induced injury in vitro and in vivo through regulating ROS/JNK/p38 signaling

“…Caffeoylquinic acid derivatives have been reported to have a strong repair effect on oxidative damage. Torres 38 reported caffeoylquinic acid derivatives inhibit oxidative stress‐induced neurotoxicity in a concentration‐dependent manner. Jho 39 reported that 3,5‐di‐ O ‐caffeoylquinic acid can reduce the oxidative damage caused by tert‐butyl hydroperoxide or paracetamol in HepG2 and reduce cell death.…”