Caffeoyl-β-d-glucopyranoside and 1,3-dihydroxy-2-tetracosanoylamino-4-(E)-nonadecene isolated from Ranunculus muricatus exhibit antioxidant activity

Abstract

This study evaluates the antioxidant activity of Ranunculus muricatus and isolation and structure elucidation of the active constituents. The aerial parts of the plants were shade dried at room temperature and powdered and extracted with methanol. The free radical scavenging activity was evaluated by 1,1-diphenyl-2-picryl-hydrazyl (DPPH) assay. The percentage scavenging activity was determined based on the percentage of DPPH radical scavenged. Column chromatography was used in order to isolate the active compounds. Spectral techniques UV, IR, 1H NMR, 13CNMR and HREI-MS were used for the structure elucidation of the isolated compounds. Two isolated compounds, A (caffeoyl-β-d-glucopyranoside) and B (1,3-dihydroxy-2-tetracosanoylamino-4-(E)-nonadecene), exibited a significant antioxidant activity as showed by DPPH radical scavenging method. Percentage inhibition for compound A (at 0.5 mM) was 82.67 ± 0.19 with IC50 of 93.25 ± 0.12 (μM), and for compound B (at 0.5 mM) was 69.23 ± 0.19 with IC50 of 183.34 ± 0.13 (μM). Quercetin was used as standard control. It was conclued from the present study that caffeoyl-β-d-glucopyranoside and 1,3-dihydroxy-2-tetracosanoylamino-4-(E)-nonadecene isolated from methanol extract of aerial parts of Ranunculus muricatus posses antioxidant activity.

The radical scavenging activity of muriolide in physiological environments: mechanistic and kinetic insights into double processes

“…7,8 The antioxidant activity of R. muricatus may be related to phenolic compounds such as avonoids, avonoid glycosides, and lactones found in the plant. 7,9,10 Muriolide (MO, Fig. 1) is a natural lactone that has been isolated from Ranunculus muricatus.…”

Photoaging Protective Effects of Ranunculus bulumei Methanol Extract

“…Ranunculus has a total of 600 species that have been reported to exhibit various medical effects. For example, Ranunculus muricatus L. has been used as a folk remedy for treating heart disease, cancer, and dental diseases [14][15][16]. Ranunculus arvensis L. has been utilized to cure arthritis, asthma, hay fever, rheumatism, psoriasis, and gut disease [17].…”

“…Ranunculus arvensis L. has been utilized to cure arthritis, asthma, hay fever, rheumatism, psoriasis, and gut disease [17]. In addition, Ranunculus muricatus L. and Ranunculus arvensis L. methanol extracts were found to contain potent antioxidants [14,17,18]. In addition, it has been reported that methanol extracts of Ranunculus peltatus subs were effective against contact dermatitis [19], and Ranunculus constantinopolitanus has anti-inflammatory activities [20].…”

4-Benzyloxylonchocarpin and Muracatanes A-C from Ranunculus muricatus L. and Their Biological Effects

“…R. muricatus is used in traditional medicine to treat cough, asthma [ 10 ], dysentery, jaundice, diarrhea, eczema, urinary infections, leprosy, and ringworm infection [ 11 , 12 ]. R. muricatus has also been employed in folk medicine to treat cancer, heart diseases, and dental diseases [ 13 , 14 , 15 ], and R. muricatus extracts have been reported to possess antioxidant [ 16 ], antifungal, antibacterial, and cytotoxic effects [ 17 ]. R. muricatus is furthermore employed to treat tonsillitis diseases in traditional medicine protocols in India [ 18 ].…”