Caffeine increased progressive motility of human spermatozoa in normozoospermic and asthenozoospermic semen samples and enhanced activity of seminal creatine kinase

Banihani, Saleem Ali; Khaled, Hebah J.

doi:10.1111/and.14052

Cited by 6 publications

(5 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

Section: Resultsmentioning

confidence: 99%

“…In andrology research, progressively motile sperm is considered the most sensitive semen quality parameters to evaluate the effect of given exogenous factors (e.g., drugs) (Banihani & Khaled, 2021). Therefore, in this study, we intended to measure the in vitro hierarchy of ranitidine drug at a range of concentrations on progressive motility of sperm.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Ranitidine decreases human sperm motility and vitality and increases the activity of seminal creatine kinase

Banihani

Al-Natsheh

2022

Andrologia

Self Cite

View full text Add to dashboard Cite

Ranitidine (brand name: Zantac), an acid reducer, belongs to histamine‐2 receptor antagonists. Since 1981, even though several adverse effects of this drug were reported in the body, still, its effects on human sperm parameters have yet to be confirmed. In this work, we attempted to measure sperm motility, sperm vitality and activity of seminal creatine kinase in the ejaculated human semen (n = 31) in the presence of ranitidine at a range of concentrations (0.1, 0.3, 0.6, 0.9 and 1.2 μg/ml) compared with control (without ranitidine). Sperm motility was measured using the Makler counter, whilst sperm vitality was assessed using the Eosin test. Creatine kinase activity was measured using the kinetic spectrophotometric method. Sperm motility (total and progressive) as well as sperm vitality was significantly (p < .05) reduced in the presence of ranitidine in human semen, particularly at the higher tested concentrations (0.6–1.2 μg/ml) compared with the control. On the other hand, creatine kinase activity was significantly increased (p < .05) in the presence of ranitidine at 0.6, 0.9 and 1.2 μg/ml. In conclusion, ranitidine at 0.6–1.2 μg/ml reduced sperm motility and vitality, but increased the activity of creatine kinase in ejaculated human semen.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Ranitidine decreases human sperm motility and vitality and increases the activity of seminal creatine kinase

Banihani

Al-Natsheh

2022

Andrologia

Self Cite

View full text Add to dashboard Cite

show abstract

“…Also, if the energy source is insufficient, motility will drop again. For instance, a previous study demonstrates that 10 nM of caffeine significantly increased the progressive motility of spermatozoa in normozoospermic and asthenozoospermic groups [49]. Studies have also shown that the caffeine content in tea increases with increasing steep time and temperature [50][51][52].…”

Section: Discussionmentioning

confidence: 96%

Black Tea Aqueous Extracts Improve Human Sperm Functions: An In Vitro Study

Setumo,

Choma,

Henkel

et al. 2023

Andrologia

View full text Add to dashboard Cite

Infertility affects about 25% of couples worldwide, and oxidative stress (OS) is linked to its idiopathic etiology. Green, black, white, and oolong teas are produced from Camellia sinensis, depending on their oxidation level, and contain antioxidant properties that may enhance male reproductive functions. The study aimed to investigate the effects of black tea aqueous extract on human sperm functions in vitro. Semen samples were collected from donors, liquefied, analyzed, and divided into normal (n = 40) and abnormal (n = 19) groups using the World Health Organization 2010 criteria. Samples were washed and incubated with black tea aqueous extracts (0, 0.4, 4, 40, 405 μg/ml) for 1 hr and analyzed. Along with a considerable decrease in intracellular reactive oxygen species (ROS) production, DNA-fragmented spermatozoa, and acrosome reaction, the percentage of sperm vitality and intact mitochondrial membrane potential (MMP) increased (p<0.05). Furthermore, compared to the normal group, a substantial increase in the percentage of acrosome reaction, ROS production, and percentage of spermatozoa with fragmented DNA, while a reduction in the percentage of intact MMP and sperm vitality, was noted in the abnormal group (p<0.05). Compared to the controls, there was no significant change in motility between the normal and abnormal groups (p>0.05). Black tea’s antioxidant activity, caffeine concentration, or both may have contributed to its improvement in human sperm function in vitro.

show abstract

“…Similarly, in the present study, we observed that the recovery rates in the inner tube were reduced to less than one‐fifth (~20%), although sufficient sperm numbers were recovered for IVF. It has been demonstrated that, in normal human sperm and azoospermia, the presence of 10 mM caffeine increases motility, whereas 0, 1, and 5 mM caffeine cause no statistical change (Banihani & Khaled, 2021). In the present study, using frozen–thawed porcine sperm, the addition of more than 5 mM caffeine increased the recovery rate; however, the addition of 10 mM caffeine decreased sperm quality, including motility.…”

Section: Discussionmentioning

confidence: 99%

Quality and fertilizing ability of frozen–thawed porcine sperm separated using a migration sedimentation method

Nguyen,

Taniguchi,

Ono

et al. 2024

Reprod Domestic Animals

View full text Add to dashboard Cite

We evaluated the quality and fertilizing ability of frozen–thawed porcine sperm that were selected using a commercially available device (MIGLIS, Menicon Life Science) consisting of three parts: an outer lid, an inner lid, and a tube. Firstly, to determine an adequate concentration of caffeine for separation, frozen–thawed sperm were incubated with different concentrations of caffeine (0, 1, 2.5, 5, and 10 mM) in a MIGLIS device. To determine the appropriate incubation time for separating sperm in the MIGLIS device, frozen–thawed sperm were incubated with 2.5 mM caffeine for 5, 10, 15, or 20 min. To evaluate the fertilization and embryo development of oocytes fertilized with frozen–thawed sperm separated into two regions (outer and inner) in the MIGLIS device, the separated sperm from the three boars was used to fertilize in vitro‐matured oocytes and cultured in vitro for 7 days. Sperm quality parameters of sperm collected from the inner tube after incubation with 2.5 mM caffeine were superior to sperm incubated without caffeine. Moreover, sperm collected from the inner tube after incubation for 10 min had a higher progressive motility. The rate of blastocyst produced from spermatozoa collected from the inner tube after incubation with 2.5 mM caffeine for 10 min significantly increased compared to that produced from spermatozoa from the outer tube, regardless of the boar. In conclusion, sperm sorting using the MIGLIS device may be useful for separating high‐quality sperm after incubation with 2.5 mM caffeine for 10 min to improve blastocyst formation.

show abstract

Caffeine increased progressive motility of human spermatozoa in normozoospermic and asthenozoospermic semen samples and enhanced activity of seminal creatine kinase

Cited by 6 publications

References 39 publications

Ranitidine decreases human sperm motility and vitality and increases the activity of seminal creatine kinase

Ranitidine decreases human sperm motility and vitality and increases the activity of seminal creatine kinase

Black Tea Aqueous Extracts Improve Human Sperm Functions: An In Vitro Study

Quality and fertilizing ability of frozen–thawed porcine sperm separated using a migration sedimentation method

Contact Info

Product

Resources

About