Caffeine Encapsulated in Small Unilamellar Liposomes: Characerization and In Vitro Release Profile

Chorilli, Marlus; Calixto, Giovana Maria Fioramonti; Rimério, Thereana Cristina; Scarpa, Maria Virgínia

doi:10.1080/01932691.2012.739535

Cited by 35 publications

(22 citation statements)

References 35 publications

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“…Clinically, they are the most established controlled drug delivery system due to their structural flexibility and their ability to incorporate a variety of hydrophilic and hydrophobic drugs, besides being biocompatible and biodegradable. Therefore, liposomes can be used to incorporate lipophilic and hydrophilic PSs used in cancer PDT [90,91].…”

Section: Nanotechnology-based Drug Delivery Systemsmentioning

confidence: 99%

Nanotechnology-Based Drug Delivery Systems for Photodynamic Therapy of Cancer: A Review

et al. 2016

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Photodynamic therapy (PDT) is a promising alternative approach for improved cancer treatment. In PDT, a photosensitizer (PS) is administered that can be activated by light of a specific wavelength, which causes selective damage to the tumor and its surrounding vasculature. The success of PDT is limited by the difficulty in administering photosensitizers (PSs) with low water solubility, which compromises the clinical use of several molecules. Incorporation of PSs in nanostructured drug delivery systems, such as polymeric nanoparticles (PNPs), solid lipid nanoparticles (SLNs), nanostructured lipid carriers (NLCs), gold nanoparticles (AuNPs), hydrogels, liposomes, liquid crystals, dendrimers, and cyclodextrin is a potential strategy to overcome this difficulty. Additionally, nanotechnology-based drug delivery systems may improve the transcytosis of a PS across epithelial and endothelial barriers and afford the simultaneous co-delivery of two or more drugs. Based on this, the application of nanotechnology in medicine may offer numerous exciting possibilities in cancer treatment and improve the efficacy of available therapeutics. Therefore, the aim of this paper is to review nanotechnology-based drug delivery systems for photodynamic therapy of cancer.

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Section: Nanotechnology-based Drug Delivery Systemsmentioning

confidence: 99%

Nanotechnology-Based Drug Delivery Systems for Photodynamic Therapy of Cancer: A Review

et al. 2016

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“…Nevertheless, this latter characteristic causes oral administration to be uncontrolled while delivery through the skin is not effective because of the lipophilic character of the skin outer layers [38]. To overcome this drawback, caffeine has been successfully encapsulated using various nanocarriers, such as liposomes [39,40], polymeric nanoparticles [41,42], and nanostructured lipid carriers [43,44]. However, scientific literature has also highlighted that caffeine encapsulation efficiency higher than 30% is rarely achieved using the above mentioned carriers, whichever the encapsulation method adopted.…”

Section: Introductionmentioning

confidence: 99%

Overcoming the Limits of Flash Nanoprecipitation: Effective Loading of Hydrophilic Drug into Polymeric Nanoparticles with Controlled Structure

et al. 2018

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Flash nanoprecipitation (FNP) is a widely used technique to prepare particulate carriers based on various polymers, and it was proven to be a promising technology for the industrial production of drug loaded nanoparticles. However, up to now, only its application to hydrophobic compounds has been deeply studied and the encapsulation of some strongly hydrophilic compounds, such as caffeine, remains a challenge. Caffeine loaded poly-ε-caprolactone (PCL) nanoparticles were produced in a confined impinging jet mixer using acetone as the solvent and water as the antisolvent. Caffeine was dissolved either in acetone or in water to assess the effects of two different process conditions. Nanoparticles properties were assessed in terms of loading capacity (LC%), encapsulation efficiency (EE%), and in vitro release kinetics. Samples were further characterized by dynamic light scattering, scanning electron microscopy, X-ray photo electron spectroscopy, and infrared spectroscopy to determine the size, morphology, and structure of nanoparticles. FNP was proved an effective technique for entrapping caffeine in PCL and to control its release behavior. The solvent used to solubilize caffeine influences the final structure of the obtained particles. It was observed that the active principle was preferentially adsorbed at the surface when using acetone, while with water, it was embedded in the matrix structure. The present research highlights the possibility of extending the range of applications of FNP to hydrophilic molecules.

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“…To determine the amount of extrusion cycles and sonication time required for the formation of the liposomes, periodic measurements were made in a spectrophotometer (Chorilli et al, 2013). The results shown in Figure 1 indicates that there is a decrease in absorbance at 410 nm as a function of sonication time and amount of extrusion cycles, respectively, for all formulations developed.…”

Section: Resultsmentioning

confidence: 99%

“…One way to overcome this problem would be the use of nanocarriers, such as liposomes, that can incorporate the peptide and increase its availability at the site of action. Liposomes have been used in therapeutics for more than 40 years, presenting many advantages, including biocompatibility, because they are constituted by phospholipids, such as the biological membranes of the cells (Chorilli et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

A Novel Antifungal System With Potential for Prolonged Delivery of Histatin 5 to Limit Growth of Candida albicans

et al. 2019

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Currently 75–88% of fungal infections are caused by Candida species, and Candida albicans is the main microorganism that causes these infections, especially oral candidiasis. An option for treatment involves the use of the antifungal peptide Histatin 5 (Hst 5), which is naturally found in human saliva but undergoes rapid degradation when present in the oral cavity, its site of action. For this reason, it is important to develop a way of applying this peptide to the oral lesions, which promotes the gradual release of the peptide. In the present study, we have evaluated the development of liposomes of different lipid compositions, loaded with the peptide as a way to promote its release slowly and gradually, preserving its antifungal potential. For this, the peptide 0WHistatin 5, an analog of the peptide Hst 5, was synthesized, which contains the amino acid tryptophan in its sequence. The solid phase synthesis method was used, followed by cleavage and purification. The liposomes were produced by thin film hydration technique in three different lipid compositions, F1, F2, and F3 and were submitted to an extrusion and sonication process to standardize the size and study the best technique for their production. The liposomes were characterized by dynamic light scattering, and tests were performed to determine the encapsulation efficiency, release kinetics, stability, and evaluation of antifungal activity. The extruded liposomes presented average size in the range of 100 nm, while sonicated liposomes presented a smaller size in the range of 80 nm. The encapsulation efficiency was higher for the sonicated liposomes, being 34.5% for F1. The sonicated F3 presented better stability when stored for 60 days at 4°C. The liposomes showed the ability to release the peptide for the total time of 96 h, with the first peak after 5 h, and a further increase of the released after 30 h. Time-kill assay showed that the liposomes were able to control yeast growth for 72 h. The data suggest that the liposomes loaded with 0WHistatin 5 maintained the action of the peptide and were able to limit the growth of C. albicans , being a suitable system for use in the treatment of oral candidiasis.

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Caffeine Encapsulated in Small Unilamellar Liposomes: Characerization and In Vitro Release Profile

Cited by 35 publications

References 35 publications

Nanotechnology-Based Drug Delivery Systems for Photodynamic Therapy of Cancer: A Review

Nanotechnology-Based Drug Delivery Systems for Photodynamic Therapy of Cancer: A Review

Overcoming the Limits of Flash Nanoprecipitation: Effective Loading of Hydrophilic Drug into Polymeric Nanoparticles with Controlled Structure

A Novel Antifungal System With Potential for Prolonged Delivery of Histatin 5 to Limit Growth of Candida albicans

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