Caffeine determination by flow injection analysis employing Bovine Serum Albumin as a fluorophore

Alesso, Magdalena; Fernández, Liliana Patricia

doi:10.1016/j.microc.2016.03.004

Cited by 9 publications

(4 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Caffeine is methylxanthine and its health influences are controversial. The primary biological influence of CA is the antagonism of adenosine receptor (Singh et al, ; Alesso & Fernández, ). The other physiological effects of CA include increased blood pressure and plasma catecholamine levels, urine production, serum fatty acid levels, and gastric acid secretion (Tefera, Geto, Tessema, & Admassie, ).…”

Section: Introductionmentioning

confidence: 99%

Extraction of caffeine and gallic acid from coffee by electrokinetic methods coupled with a hollow‐fiber membrane

Khajeh

Fard

Bohlooli

et al. 2017

J Food Process Engineering

View full text Add to dashboard Cite

In this study the hollow fiber membrane coupled with electrokinetics was used, for the first time, for extraction and determination of caffeine and gallic acid from coffee prior to high performance liquid chromatography with photodiode array detection. Gallic acid (as an acid) and caffeine (as a base) were extracted from coffee at pH 5. In this work, several variables including applied voltage, coffee pH, acceptor phase pH, and extraction time were investigated. Our results showed that the extraction efficiency was increased when the applied voltage and extraction time were increased. The optimum conditions were pH 5 for the coffee, applied voltage of 20 V, and extraction time of 5 h. Gallic acid as an acid and caffeine as a base migrated to anode and cathode, respectively. Under the optimum conditions, gallic acid and caffeine were extracted with extraction efficiency of 21.3 and 23.4%, respectively. The intra‐day and inter‐day precisions (%) were found to be 7.6 and 4.8%, respectively. Therefore, this set‐up for electrokinetics was potentially considered as a selective and simple preparation method for simultaneous extraction and analysis of basic and acid compounds in various matrices. Practical applications This study would benefit for extraction and separation of caffeine and gallic acid from coffee and other naturel samples. In this procedure, the new set‐up for EKE based on two hollow fibers were employed for simultaneous removal of basic and acidic compounds. The EKE is used for extraction of organic compounds from food/soils with low permeability. The practical applications of this procedure have become extensive in a variety of fields, including electrokinetic waste remediation, biomaterials, radionuclides, and organic materials.

show abstract

Section: Introductionmentioning

confidence: 99%

Extraction of caffeine and gallic acid from coffee by electrokinetic methods coupled with a hollow‐fiber membrane

Khajeh

Fard

Bohlooli

et al. 2017

J Food Process Engineering

View full text Add to dashboard Cite

show abstract

“…Several parameters were optimized such as the nature and the concentration of both the buffer and the fluorophore, and the carrier flow rate, leading to a linear range from 6.68 × 10 −6 to 4.0 × 10 −3 molL −1 . This sensitive and selective method was successfully employed for caffeine determination from various matrices such as energy drinks, dietary supplements, and slimming infusion samples without any pretreatment [45].…”

Section: Spectroscopic Methodsmentioning

confidence: 99%

Coffea arabica: A Plant with Rich Content in Caffeine

Patay¹,

Fritea²,

Antonescu³

et al. 2017

The Question of Caffeine

View full text Add to dashboard Cite

Coffea arabica L. is the most well-known and studied Coffea taxa, which is very popular in both scientific and social fields. This comprehensive work was created in order to describe its phytochemical composition and to present the metabolism of caffeine, which is the most important alkaloid from this plant. The analytical methods used for caffeine determination such as chromatographic, electrochemical, and spectroscopic techniques are also presented. In addition, this work emphasizes the medicinal importance of caffeine, which can present both important beneficial and secondary effects for human body.

show abstract

“…Its consumption provides enhance on the performance of human body since it acts as stimulant for the cardiac muscle, gastric secretion, diuresis, central nervous, and respiratory systems [3–5]. Due to all those advantages, caffeine is consumed in higher quantities for athletes to enhance their physical performance and mental aptitude; however, the exceeding use of caffeine is considered doping and it is forbidden by the World Anti-Doping Agency (WADA) [6].…”

Section: Introductionmentioning

confidence: 99%

“…Significant increase in the risk of low birth weight is associated with a high caffeine intake during pregnancy. Additionally, dependence, tolerance, tremor, anxiety, insomnia, weakness, palpitations, gastrointestinal disturbances, and increased blood pressure are some of the disorders caused by ingesting caffeine in large amounts [6, 7]. The consumption of caffeine above 200 mg/day may lead to death, since this quantity is already considered toxic [7].…”

Section: Introductionmentioning

confidence: 99%

Electrodeposition of 4-Benzenesulfonic Acid onto a Graphite-Epoxy Composite Electrode for the Enhanced Voltammetric Determination of Caffeine in Beverages

Furtado

Gonçalves

Inocêncio

et al. 2019

Journal of Analytical Methods in Chemistry

View full text Add to dashboard Cite

Caffeine is widely present in food and drinks, such as teas and coffees, being also part of some currently commercialized medicines, but despite its enhancement on several functions of human body, its exceeding use can promote many health problems. In order to develop new fast approaches for the caffeine sensing, graphite-epoxy composite electrodes (GECE) were used as substrate, being modified by different diazonium salts, synthetized as their tetraflouroborate salts. An analytical method for caffeine quantification was developed, using sware wave voltammetry (SWV) in Britton–Robinson buffer pH 2.0. Detection limits for bare electrode and 4-benzenesulfonic modified electrode were observed circa 145 µmol·L−1 and 1.3 µmol·L−1, respectively. The results have shown that the modification shifts the oxidation peaks to lower potential. Kinetics of the reaction limited by diffusion was more expressive when caffeine was added to the solution, resulting in decreases of impedance, characterized by lower Rct. All results for caffeine determination were compared to a reference chromatographic procedure (HPLC), showing no statistical difference. Analytical parameters for validation were suitably determined according to local legislation, leading to a linear behaviour from 5 to 150 µmol·L−1; precision of 4.09% was evaluated based on the RDC 166/17, and accuracy was evaluated in comparison with the reference method, with recovery of 98.37 ± 2.58%.

show abstract

Caffeine determination by flow injection analysis employing Bovine Serum Albumin as a fluorophore

Cited by 9 publications

References 27 publications

Extraction of caffeine and gallic acid from coffee by electrokinetic methods coupled with a hollow‐fiber membrane

Extraction of caffeine and gallic acid from coffee by electrokinetic methods coupled with a hollow‐fiber membrane

Coffea arabica: A Plant with Rich Content in Caffeine

Electrodeposition of 4-Benzenesulfonic Acid onto a Graphite-Epoxy Composite Electrode for the Enhanced Voltammetric Determination of Caffeine in Beverages

Contact Info

Product

Resources

About