Caffeine delays oocyte aging and maintains the quality of aged oocytes safely in mouse

Zhang, Xia; Liu, Xiaoyan; Chen, Li; Wu, Dan-Ya; Nie, Zheng‐Wen; Gao, Yingying; Miao, Yi‐Liang

doi:10.18632/oncotarget.15292

Cited by 22 publications

(11 citation statements)

References 31 publications

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“…Postovulatory ageing in mammals occurs between ovulation of mature oocytes and their fertilization and has been shown to decrease oocytes’ ability to react properly to sperm penetration and to develop further into the embryo 1–8 . Aged oocytes display a wide range of various abnormalities, such as dysfunction of actomyosin and microtubular cytoskeleton 8–13 , decreased activity of M-phase promoting kinases 11,14,15 , deregulation of energy metabolism 2,16–18 and epigenetic alterations 19,20 . The extent of these abnormalities depends on the length of ageing period and, most likely, the genetic background of the cell.…”

Section: Introductionmentioning

confidence: 99%

Postovulatory ageing modifies sperm-induced Ca2+ oscillations in mouse oocytes through a conditions-dependent, multi-pathway mechanism

Szpila

Walewska

Sabat-Pośpiech

et al. 2019

Sci Rep

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Postovulatory ageing of mammalian oocytes occurs between their ovulation and fertilization and has been shown to decrease their developmental capabilities. Aged oocytes display numerous abnormalities, including altered Ca 2+ signalling. Fertilization-induced Ca 2+ oscillations are essential for activation of the embryonic development, therefore maintaining proper Ca 2+ homeostasis is crucial for the oocyte quality. In the present paper, we show that the mechanism underlying age-dependent alterations in the pattern of sperm-triggered Ca 2+ oscillations is more complex and multifaceted than previously believed. Using time-lapse imaging accompanied by immunostaining and molecular analyses, we found that postovulatory ageing affects the amount of Ca 2+ stored in the cell, expression of Ca 2+ pump SERCA2, amount of available ATP and distribution of endoplasmic reticulum and mitochondria in a manner often strongly depending on ageing conditions ( in vitro vs. in vivo ). Importantly, those changes do not have to be caused by oxidative stress, usually linked with the ageing process, as they occur even if the amount of reactive oxygen species remains low. Instead, our results suggest that aberrations in Ca 2+ signalling may be a synergistic result of ageing-related alterations of the cell cycle, cytoskeleton, and mitochondrial functionality.

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Section: Introductionmentioning

confidence: 99%

Postovulatory ageing modifies sperm-induced Ca2+ oscillations in mouse oocytes through a conditions-dependent, multi-pathway mechanism

Szpila

Walewska

Sabat-Pośpiech

et al. 2019

Sci Rep

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“…The authors attributed their findings to the prolonged period of meiotic arrest of vitrified MII oocytes in caffeine treated group [51]. It has been documented that caffeine could maintain the quality of aged oocytes safely in mouse as aged oocytes treated by caffeine has a potential to be activated [55]. In mice, supplementation of vitrification and warming media with 10 mM caffeine significantly improved blastocyst rates after IVF of MII-oocytes [42].…”

Section: Discussionmentioning

confidence: 99%

Caffeine and oocyte vitrification: Sheep as an animal model

Moawad

Choi

Zhu

et al. 2018

International Journal of Veterinary Science and Medicine

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Oocyte cryopreservation is valuable way of preserving the female germ line. Vitrification of immature ovine oocytes decreased the levels of both maturation promoting factor (MPF) and mitogen-activated protein kinase (MAPK) in metaphase II (MII) oocytes after IVM. Our aims were 1) to evaluate the effects of vitrification of ovine GV-oocytes on spindle assembly, MPF/MAP kinases activities, and preimplantation development following IVM and IVF, 2) to elucidate the impact of caffeine supplementation during IVM on the quality and development of vitrified/warmed ovine GV-oocytes. Cumulus-oocyte complexes (COCs) from mature ewes were divided into vitrified, toxicity and control groups. Oocytes from each group were matured in vitro for 18 h in caffeine free IVM medium and denuded oocytes were incubated in maturation medium supplemented with 10 mM (+) or without (−) caffeine for another 6 h. At 24 h.p.m., oocytes were evaluated for spindle configuration, MPF/MAP kinases activities or fertilized and cultured in vitro for 7 days. Caffeine supplementation did not significantly affect the percentages of oocytes with normal spindle assembly in all the groups. Caffeine supplementation during IVM did not increase the activities of both kinases in vitrified groups. Cleavage and blastocyst development were significantly lower in vitrified groups than in control. Caffeine supplementation during the last 6 h of IVM did not significantly improve the cleavage and blastocyst rates in vitrified group. In conclusion, caffeine treatment during in vitro maturation has no positive impact on the quality and development of vitrified/warmed ovine GV-oocytes after IVM/IVF and embryo culture.

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“…All the results elucidate that the “window” for optimal fertilization can be extended. Meanwhile, it should be noted that caffeine treatment to mouse oocytes could cause zona pellucida hardened which reduced blastocyst rate [79], the result clearly indicates that caffeine treatment may cause some side-effects on the developmental potential in certain species. Therefore, further investigation to the effects of the inhibitors on ovine oocytes is required.…”

Section: Extension Of Fertile Span Of Ovine Oocytes Matured In Vitromentioning

confidence: 99%

Advances in in vitro production of sheep embryos

Zhu

Moawad

Wang

et al. 2018

International Journal of Veterinary Science and Medicine

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Sheep is an important livestock in the world providing meat, milk and wool for human beings. With increasing human population, the worldwide needs of production of sheep have elevated. To meet the needs, the assistant reproductive technology including ovine in vitro embryo production (ovine IVP) is urgently required to enhance the effective production of sheep in the world. To learn the status of ovine IVP, we collected some publications related to ovine IVP through PubMed and analyzed the progress in ovine IVP made in the last five years (2012–2017). We made comparisons of these data and found that the recent advances in ovine IVP has been made slowly comparable to that of ovine IVP two decades ago. Therefore, we suggested two strategies or approaches to tackle the main problems in ovine IVP and expect that the efficiency of ovine IVP could be improved significantly when the approaches would be implemented.

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Caffeine delays oocyte aging and maintains the quality of aged oocytes safely in mouse

Cited by 22 publications

References 31 publications

Postovulatory ageing modifies sperm-induced Ca2+ oscillations in mouse oocytes through a conditions-dependent, multi-pathway mechanism

Postovulatory ageing modifies sperm-induced Ca2+ oscillations in mouse oocytes through a conditions-dependent, multi-pathway mechanism

Caffeine and oocyte vitrification: Sheep as an animal model

Advances in in vitro production of sheep embryos

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