Caffeic acid treatment alters the extracellular adenine nucleotide hydrolysis in platelets and lymphocytes of adult rats

Abstract

This study evaluated the effects of caffeic acid on ectonucleotidase activities such as NTPDase (nucleoside triphosphate diphosphohydrolase), Ecto-NPP (nucleotide pyrophosphatase/phosphodiesterase), 5'-nucleotidase and adenosine deaminase (ADA) in platelets and lymphocytes of rats, as well as in the profile of platelet aggregation. Animals were divided into five groups: I (control); II (oil); III (caffeic acid 10 mg/kg); IV (caffeic acid 50 mg/kg); and V (caffeic acid 100 mg/kg). Animals were treated with caffeic acid diluted in oil for 30 days. In platelets, caffeic acid decreased the ATP hydrolysis and increased ADP hydrolysis in groups III, IV and V when compared to control (P<0.05). The 5'-nucleotidase activity was decreased, while E-NPP and ADA activities were increased in platelets of rats of groups III, IV and V (P<0.05). Caffeic acid reduced significantly the platelet aggregation in the animals of groups III, IV and V in relation to group I (P<0.05). In lymphocytes, the NTPDase and ADA activities were increased in all groups treated with caffeic acid when compared to control (P<0.05). These findings demonstrated that the enzymes were altered in tissues by caffeic acid and this compound decreased the platelet aggregation suggesting that caffeic acid should be considered a potentially therapeutic agent in disorders related to the purinergic system.

Dietary Supplementation of Ginger and Turmeric Rhizomes Modulates Platelets Ectonucleotidase and Adenosine Deaminase Activities in Normotensive and Hypertensive Rats

“…Curcumin as well as other phenolics like caffeic acid, reservatrol and others has been reported to exert antiplatelet activity and also modulates ectonucleotidase activities from platelets (Olas and Wachowicz, ; Olas et al, ; Schmatz et al, ; Anwar et al, ). Many other phenolic compounds can modulate ectonucleotidase and inhibit ADA activities (Olas and Wachowicz, ; Olas et al, ; Schmatz et al, ; Anwar et al, ). Thus, this suggests that this effect exhibited by both rhizomes could be attributed to the phenolics characterized in the rhizomes (Fig.…”

Simultaneous determination of caffeic acid and its major pharmacologically active metabolites in rat plasma by LC‐MS/MS and its application in pharmacokinetic study

“…As a natural product, it possesses several biological and physiological activities. These activities include antioxidant (Kikuzaki et al, 2002;Gulcin, 2006;Anwar et al, 2013), anti-mutagenic (Yamada and Tomita, 1996), anti-tumor (Huang et al, 1988;Chung et al, 2004;Cho et al, 2010) and anti-obesity effects (Piazzon et al, 2012). Caffeic acid has been developed into drug for sale.…”

Evaluation of the antiaggregant activity of ascorbyl phenolic esters with antioxidant properties

“…Recently, the negative role of caffeic acid was attributed to its effects on the activity of ectonucleotidases, such as 5′-nucleotidase and adenosine deaminase [3]; as a result, the presence of caffeic acid resulted in a decrease in ATP hydrolysis and enhanced ADP hydrolysis and phenolic acid uptake by platelets, which reduced platelet aggregation [46]. Hence, we consider that the aggregation inhibition observed in the presence of Caf Asc might be due to the combined antioxidant action of the precursor molecules and the oxidative-independent reduction in the ADP content.…”

“…They have also been reported to affect platelet function. For instance, gallic acid, caffeic acid, and protocatechuic acid, being the latest found in roselle, onion skin, and mushrooms, showed that antiplatelets effect through altering different platelet signaling pathways [3,11,33,50].…”