Caffeic Acid Phenethyl Ester Suppresses the Production of Adipocytokines, Leptin, Tumor Necrosis Factor -Alpha and Resistin, during Differentiation to Adipocytes in 3T3-L1 Cells

Juman, Sachiko; Yasui, Naomi; Okuda, Hiroto; Ueda, Ai; Negishi, Hiroko; Miki, Takuji; Ikeda, Katsumi

doi:10.1248/bpb.34.490

Cited by 28 publications

(34 citation statements)

References 28 publications

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“…Adipocyte differentiation of the cells was facilitated according to an established protocol. 24) Briefly, 3T3-L1 cells were plated in 6-well plates, 24-well plates, or 35 mm dishes, and grown to confluence in DMEM with 10% CS. When the cells were confluent, they were fed DMEM supplemented with 10% fetal bovine serum (FBS; Biowest; Nuaille, France), 1 µM dexametazone (DEX; Sigma), 500 µM isobutylmethylxanthine (IBMX; Sigma), 5 µg/mL insulin (Wako Pure Chemical Industries, Ltd., Osaka, Japan), and various concentrations (10,25, and 50 µg/mL) of SJW extract or DMSO as a vehicle control (Day 0), and cultured for 3 d. After 3 d (Day 3), the medium was replaced with DMEM containing 10% FBS, 5 µg/mL insulin, and SJW or DMSO.…”

Section: Methodsmentioning

confidence: 99%

St. John’s Wort Promotes Adipocyte Differentiation and Modulates NF-κB Activation in 3T3-L1 Cells

Hatano

Sameshima

Kawabata

et al. 2014

Biological & Pharmaceutical Bulletin

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St. John's wort (SJW), or Hypericum perforatum, is a perennial herb that has been used in the treatment of depression in several countries. Though its therapeutic effect on depression has been extensively studied, its influence on metabolic syndrome is yet to be fully characterized. Therefore, we investigated the effect of SJW extract on adipocyte differentiation and its anti-inflammatory effects by using 3T3-L1 preadipocytes. Oil Red O staining indicated that SJW promotes adipocyte differentiation, while immunoblots indicated that SJW increases the expression of peroxisome proliferator activated receptor γ (PPARγ), a nuclear receptor regulating adipocyte differentiation, and adiponectin, an anti-inflammatory adipokine. Furthermore, the anti-inflammatory activity of SJW was demonstrated by its inhibition of the activation of nuclear factor-κB (NF-κB), an inflammatory transcription factor. Stimulation of mature 3T3-L1 adipocytes by tumor necrosis factor-α (TNF-α) decreased the expression of the NF-κB inhibitor IκBα, and increased its phosphorylation. Treatment with SJW further decreased the TNF-α-induced perturbation in IκBα expression and phosphorylation, which indicated that SJW mediated the inhibition of NF-κB activation. In addition, SJW decreased the TNF-α-induced increase in the mRNA levels of pro-inflammatory adipokines, interleukin-6 (IL-6), and monocyte chemoattractant protein-1 (MCP-1). Collectively, our results indicate that SJW treatment could promote adipocyte differentiation probably through its anti-inflammatory activity, which in turn suggests that SJW has the potential to minimize the risk factors of metabolic syndrome.

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Section: Methodsmentioning

confidence: 99%

St. John’s Wort Promotes Adipocyte Differentiation and Modulates NF-κB Activation in 3T3-L1 Cells

Hatano

Sameshima

Kawabata

et al. 2014

Biological & Pharmaceutical Bulletin

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“…However, besides their antioxidants activity, polyphenols may function in other ways, namely by: (i) reducing inflammatory response by blocking proinflammatory cytokines and transcription factors involved in metabolic diseases, or by activating transcription factors that antagonize chronic inflammation [38,41,213,[256][257][258], (ii) improving insulin sensitivity and chronic inflammation [211,212,[259][260][261], (iii) ameliorating endothelial function through the normalization of vascular tone and an overall antihypertensive effect [262], (iv) acting as phytoestrogens [263][264][265]. Thus dietary polyphenols participate in a wide spectrum of biological processes potentially beneficial for health by interacting with several cellular components and molecular pathways [41,231,257,266] as well as with obesity-related genes including adipocytokine [267][268][269][270] orexigenic, anorectic, and energy expenditure genes [271]. Moreover, there is now considerable evidence that various dietary exposures occurring during critical time windows of development-e.g.…”

Section: Biological Effects Of Polyphenolsmentioning

confidence: 99%

Management of reproduction and pregnancy complications in maternal obesity: Which role for dietary polyphenols?

et al. 2013

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Obesity is a global and dramatic public health problem; maternal obesity represents one of the main risk factors of infertility and pregnancy complications as it is associated with adverse maternal and offspring outcomes. In the last few years, adipose tissue dysfunction associated with altered adipocytokine secretion has been suggested to play a critical role in all the phases of reproductive process. Obesity is a nutrition-related disorder. In this regard, dietary intervention strategies, such as high intake of fruit and vegetables, have shown significant effects in both preserving health and counteracting obesity-associated diseases. Evidence has been provided that polyphenols, important constituents of plant-derived food, can influence developmental program of oocyte and embryo, as well as pregnancy progression by modulating several cellular pathways. This review will examine the controversial results so far obtained on adipocytokine involvement in fertility impairment and pregnancy complications. Furthermore, the different effects exerted by polyphenols on oocyte, embryo, and pregnancy development will be also taken in account.

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“…We have reported that CAPE has suppression effect on 3T3-L1 differentiation to adipocytes and production of adipocytokines during adipogenesis. 20,21) In the present study, we investigated the inhibitory effect of CAPE on LPS-induced production of pro-inflammatory cytokines in a macrophage cell line, RAW264.7, and the suppressive effect of CAPE on 3T3-L1 hypertrophic adipocytes, which are stimulated by pro-inflammatory cytokines present in the supernatant from RAW264.7 macrophages. 4 cells/100 µL/well.…”

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Caffeic Acid Phenethyl Ester Suppresses the Production of Pro-inflammatory Cytokines in Hypertrophic Adipocytes through Lipopolysaccharide-Stimulated Macrophages

Juman

Yasui

Ikeda

et al. 2012

Biological & Pharmaceutical Bulletin

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Obesity is a condition in which excess body fat accumulates due to lipids producing adipocytes and an increased number of differentiated mature cells. Recently, new findings have shown that macrophages infiltrate into adipose tissues and produce various pro-inflammatory cytokines in obese subjects. The inflammatory changes induced by the cross-talk between adipocytes and macrophages are critical for the pathophysiology of obesity and thus of metabolic syndrome. Caffeic acid phenethyl ester (CAPE) is known to have many functions, including antibacterial, anticancer and anti-inflammatory properties, but there is no evidence of its effect on the inflammatory responses in hypertrophic adipocytes through stimulation by macrophages. We investigated the effect of CAPE on macrophages and hypertrophic adipocytes in this study. CAPE significantly suppressed the levels of lipopolysaccharide (LPS)-induced interleukin (IL)-1-beta, tumor necrosis factor (TNF)-alpha and monocyte chemoattractant protein (MCP)-1 from a macrophage cell line, RAW264.7. Supernatants of stimulated RAW264.7 macrophages drastically increased mRNA levels of pro-inflammatory cytokines such as IL-6, MCP-1 and TNF-alpha in 3T3-L1 hypertrophic adipocytes. CAPE also significantly and dose-dependently reduced the gene expression of these cytokines. Our findings indicate that CAPE has inhibitory effects on the production of pro-inflammatory cytokines from LPS-stimulated RAW264.7 macrophages. In addition, CAPE suppressed gene expressions of cytokines under inflammatory conditions of hypertrophic adipocytes, suggesting that it may have the potential to suppress inflammation by macrophage infiltration into adipose tissue in obese patients.Key words caffeic acid phenethyl ester; hypertrophic adipocyte; macrophage; pro-inflammatory cytokine Obesity is a major risk factor for metabolic syndrome, which includes a number of disorders, such as hypertension, heart diseases and diabetes.1,2) Obesity is a condition in which excess body fat accumulates due to lipids producing hypertrophic adipocytes and an increased number of differentiated mature cells. This process is regulated by genetic and environmental factors, such as nutrient intake.3,4) It is known that adipocytes in obese individuals show cell hypertrophy and chronic inflammation. The inflammatory changes induced by the cross-talk between adipocytes and macrophages are critical for the pathophysiology of obesity and thus of metabolic syndrome. 5,6) Recent studies have demonstrated that obese adipose tissue is characterized by increased infiltration of macrophages, 7,8) which produce various inflammatory proteins. The inflammation process is associated with the activation of macrophages and the release of pro-inflammatory cytokines, such as interleukin (IL)-1-beta, tumor necrosis factor (TNF)-alpha, monocyte chemoattractant protein (MCP)-1 and interleukin (IL)-6. 9,10) MCP-1, known to play a pivotal role in macrophage trafficking and activation, is produced by macrophages and adipocytes. Several reports suggest t...

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Caffeic Acid Phenethyl Ester Suppresses the Production of Adipocytokines, Leptin, Tumor Necrosis Factor -Alpha and Resistin, during Differentiation to Adipocytes in 3T3-L1 Cells

Cited by 28 publications

References 28 publications

St. John’s Wort Promotes Adipocyte Differentiation and Modulates NF-κB Activation in 3T3-L1 Cells

St. John’s Wort Promotes Adipocyte Differentiation and Modulates NF-κB Activation in 3T3-L1 Cells

Management of reproduction and pregnancy complications in maternal obesity: Which role for dietary polyphenols?

Caffeic Acid Phenethyl Ester Suppresses the Production of Pro-inflammatory Cytokines in Hypertrophic Adipocytes through Lipopolysaccharide-Stimulated Macrophages

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