Caffeic acid modulates ultraviolet radiation-B induced oxidative damage in human blood lymphocytes

Prasad, Nagarajan Rajendra; Jeyanthimala, Kasinathan; Samivel, Ramachandran

doi:10.1016/j.jphotobiol.2009.03.007

Cited by 85 publications

(65 citation statements)

References 52 publications

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“…The outcomes of treatment were evaluated using the apoptosis/necrosis assay, the alkaline comet assay, and the cytokinesis-block micronucleus (CBMN) cytome assay. All of these methods are wellestablished in genetic toxicology and have proven useful in studies with different plant extracts or their active constituents (18)(19)(20)(21)(22)(23)(24)(25)(26).…”

mentioning

confidence: 99%

In vitro assessment of the cytotoxic, DNA damaging, and cytogenetic effects of hydroquinone in human peripheral blood lymphocytes

Jurica

Karačonji

Benković

et al. 2017

Archives of Industrial Hygiene and Toxicology

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This study investigated the mechanisms of hydroquinone toxicity and assessed the relationships between its cytotoxic, genotoxic, and cytogenetic effects tested at 8, 140, and 280 µg mL -1 in human peripheral blood lymphocytes exposed for 24 h. The outcomes of the treatments were evaluated using the apoptosis/necrosis assay, the alkaline comet assay, and the cytokinesis-block micronucleus (CBMN) cytome assay. The tested hydroquinone concentrations produced relatively weak cytotoxicity in resting lymphocytes, which mostly died via apoptosis. Hydroquinone's marked genotoxic effects were detected using the alkaline comet assay. Significantly decreased values of all comet parameters compared to controls indicated specific mechanisms of hydroquinone-DNA interactions. Our results suggest that the two higher hydroquinone concentrations possibly led to cross-linking and adduct formation. Increased levels of DNA breakage measured following exposure to the lowest concentration suggested mechanisms related to oxidative stress and inhibition of topoisomerase II. At 8 µg mL -1 , hydroquinone did not significantly affect MN formation. At 140 and 280 µg mL -1 , it completely blocked lymphocyte division. The two latter concentrations also led to erythrocyte stabilization and prevented their lysis. At least two facts contribute to this study's relevance: (I) this is the first study that quantifies the degree of reduction in total comet area measured in lymphocyte DNA after hydroquinone treatment, (II) it is also the first one on a lymphocyte model that adopted the "cytome" protocol in an MN assay and found that lymphocytes exposure even to low hydroquinone concentration resulted in a significant increase of nuclear bud frequency. Considering the limitations of the lymphocyte model, which does not possess intrinsic metabolic activation, in order to unequivocally prove the obtained results further studies using other appropriate cell lines are advised.

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mentioning

confidence: 99%

In vitro assessment of the cytotoxic, DNA damaging, and cytogenetic effects of hydroquinone in human peripheral blood lymphocytes

Jurica

Karačonji

Benković

et al. 2017

Archives of Industrial Hygiene and Toxicology

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“…Furthermore, it was demonstrated that EGCG has a UV-protective eff ect in human dermal fi broblasts (15). CA and QC are known to exert inhibitory eff ects against UV-induced oxidative stress (16,17). As shown in Fig.…”

Section: Establishing a System To Identify Novel Reagents Regulating mentioning

confidence: 96%

“…Other reports showed that EGCG reduced UVB-induced oxidative DNA damage in living skin equivalents (18). Although previous reports demonstrated CA-and QC-mediated anti-oxidative eff ects in hairless mice and lymphocytes, these eff ects might be cell-specifi c or may not be directly involved in the loss of cell viability mediated by UV radiation (16,17). Overall, our results indicate that the DREAM system is useful for the identifi cation of novel reagents regulating UV-induced DNA damage in human dermal fi broblasts.…”

Section: Establishing a System To Identify Novel Reagents Regulating mentioning

confidence: 98%

Development of a high-throughput screening system for identification of novel reagents regulating DNA damage in human dermal fibroblasts

Bae

2015

Acta Pharmaceutica

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Development of a high-throughput screening system for identifi cation of novel reagents regulating DNA damage in human dermal fi broblasts Ultraviolet (UV) radiation is a major inducer of skin aging and accumulated exposure to UV radiation increases DNA damage in skin cells, including dermal fi broblasts. In the present study, we developed a novel DNA repair regulating material discovery (DREAM) system for the high-throughput screening and identifi cation of putative materials regulating DNA repair in skin cells. First, we established a modifi ed lentivirus expressing the luciferase and hypoxanthine phosphoribosyl transferase (HPRT) genes. Then, human dermal fi broblast WS-1 cells were infected with the modifi ed lentivirus and selected with puromycin to establish cells that stably expressed luciferase and HPRT (DREAM-F cells).The fi rst step in the DREAM protocol was a 96-well-based screening procedure, involving the analysis of cell viability and luciferase activity aft er pretreatment of DREAM-F cells with reagents of interest and post-treatment with UVB radiation, and vice versa. In the second step, we validated certain eff ective reagents identifi ed in the fi rst step by analyzing the cell cycle, evaluating cell death, and performing HPRT-DNA sequencing in DREAM-F cells treated with these reagents and UVB. This DREAM system is scalable and forms a time-saving high-throughput screening system for identifying novel anti-photoaging reagents regulating DNA damage in dermal fi broblasts.Keywords: human dermal fi broblasts, DNA damage, high --throughput screening, agingThe skin is the largest organ and forms the outermost layer of the human body. Therefore, the skin is more easily exposed to toxic environmental agents, particularly ultraviolet (UV) radiation, than are other tissues, except for the eyes. Human skin consists of the epidermis, dermis and hypodermis. The most abundant cells in the dermis layer are human dermal fi broblasts (HDFs), which contribute to skin fi rmness and elasticity by upregulating

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“…Common compounds and effects specific to each are mentioned below. * Hydroxicinnamic acids such ascaffeic or ferulic acids prevent UVB-induced erythema in vivo and in vitro, and decrease UVinduced oxidative damage in skin cells and lymphocytes [10][11][12][13]. * Polyphenolics such as flavonoids and phenolic acids, green tea polyphenols, resveratrol, astaxanthin have been found useful [14].…”

Section: Antioxidantsmentioning

confidence: 99%

Sunscreens and Antioxidants as Photo-protective Measures: An update

Hassan¹,

Dorjay²,

Sami³

et al. 2013

Our Dermatol Online

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There are many photo-protective measures adopted for protection from the solar radiation especially the UV radiation spectrum, sunscreens being the main agents. Besides the traditional approach of topical use of sunscreens, both chemical and physical, a new approach has emerged to use systemic agents in the form of vitamins and minerals. In this review, we are describing the major aspects related to sunscreens and anti-oxidants as photo-protective measures.

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Caffeic acid modulates ultraviolet radiation-B induced oxidative damage in human blood lymphocytes

Cited by 85 publications

References 52 publications

In vitro assessment of the cytotoxic, DNA damaging, and cytogenetic effects of hydroquinone in human peripheral blood lymphocytes

In vitro assessment of the cytotoxic, DNA damaging, and cytogenetic effects of hydroquinone in human peripheral blood lymphocytes

Development of a high-throughput screening system for identification of novel reagents regulating DNA damage in human dermal fibroblasts

Sunscreens and Antioxidants as Photo-protective Measures: An update

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