Caffeic acid inhibits organic anion transporters OAT1 and OAT3 in rat kidney

Uwai, Yuichi; Kawasaki, Tatsuya; Nabekura, Tomohiro

doi:10.1515/dmdi-2013-0050

Cited by 9 publications

(9 citation statements)

References 14 publications

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“…Here, the active secretion of 6-CF was inhibited by probenecid and d -malate, suggesting that it was mediated by renal rOat1 and rOat3. Phenolsulfonphthalein is often used to investigate renal organic anion transport in vivo . ,,, For example, we previously examined the effects of lithium on the renal organic anion transport using phenolsulfonphthalein . Sakurai et al used phenolsulfonphthalein for cellular study, but the determination of phenolsulfonphthalein by an absorption spectrometer showed a lower limit of detection of several μM.…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, the renal handling of the flavonoid and its effect on renal transporters is an important issue from the standpoint of nutritional research. For OAT1 and OAT3, interactions with various dietary components, such as flavonoids and their conjugates, phenolic acids, and phenylpropanoids, are demonstrated by the inhibition of OAT-mediated transport of model substrates. − We previously investigated the effects of natural compounds on OAT1 and OAT3 function using human OAT1- and OAT3-expressing cells and the fluorescent organic anion 6-carboxyfluorescein (6-CF) and found that several phenylpropanoids inhibit OAT1 and OAT3 functions …”

Section: Introductionmentioning

confidence: 99%

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(−)-Epigallocatechin-3-gallate Inhibits Human and Rat Renal Organic Anion Transporters

et al. 2021

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Organic anion transporter 1 (OAT1, SLC22A6 ) and 3 (OAT3, SLC22A8 ) are multispecific drug transporters highly expressed on the basolateral membranes of the renal proximal tubules. OAT1 and OAT3 mediate the tubular secretion of clinically significant drugs; thus, they influence the pharmacokinetics of drugs and further determine their efficacy and toxicity. OAT1 and OAT3 are also the target of drug–drug interactions. In this study, we examined the effects of the tea catechin (−)-epigallocatechin-3-gallate (EGCG) on human (h) and rat (r) OAT1 and OAT3 using the fluorescent organic anion 6-carboxyfluorescein (6-CF) and hOAT1-, hOAT3-, rOat1-, or rOat3-expressing HEK293 cells and on renal elimination of 6-CF in rats. 6-CF is transported by hOAT1, hOAT3, rOat1, and rOat3. 6-CF is urinary excreted by Oats in rats. EGCG, a dominant catechin in green tea leaf, inhibits human and rat OAT1 and OAT3 and reduces the renal elimination of 6-CF in rats. Our findings are useful for the assessment of food–drug interactions mediated by renal OATs.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

(−)-Epigallocatechin-3-gallate Inhibits Human and Rat Renal Organic Anion Transporters

et al. 2021

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“…It has also been reported that caffeic acid inhibits organic anion transporters (OAT1 and OAT3) in rat kidneys. [42] However, there has been no report on the relationship between caffeic acid and organic cation transporter (OCT), and OCTs have a close relationship with the entry of CDDP into kidney cells [43]. Phenylacetylglycine may be a suitable biomarker associated with mitochondrial toxicity [44].…”

Section: Discussionmentioning

confidence: 99%

Metabolomics study of fasudil on cisplatin-induced kidney injury

Xia

Lai

et al. 2019

Bioscience Reports

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Fasudil is a derivative of 5-isoquinoline sulfonamide, which is a Rho kinase inhibitor, a wide range of pharmacological effects. Fasudil has been shown to attenuate kidney injury caused by certain substances. In the present study, metabolomic analysis of mouse kidney tissues ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry was used to determine the metabolomic changes in cisplatin-induced kidney injury and the fasudil-induced attenuation of cisplatin-induced kidney injury. Metabolomic profiling of kidney tissues revealed significant differences in metabolites between the control group and the cisplatin group and between the cisplatin group and the fasudil-intervention group. With metabolomic approach, 68 endogenous differential metabolites were found, and multivariate statistical analysis, accurate molecular weights, isotope tracers, mass-spectrometry secondary-fragment information, and standard-reference comparisons were used to identify these substances. Based on these differential metabolites, a metabolic-pathway network was constructed and revealed that fasudil primarily attenuated cisplatin-induced renal injury by modulating lipid and amino-acid metabolism. These results further demonstrate that kidney injury can be induced by cisplatin and, moreover, suggest that fasudil can be used to reduce kidney injury at early stages in patients treated with cisplatin.

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“…Previous studies have demonstrated that caffeic acid from coffee, fruits, and vegetables significantly inhibits OAT3 (IC 50 = 5.4 μM) [ 71 ]. Yuichi et al uncovered that caffeic acid inhibits substrate uptake by OAT3 in a concentration-dependent manner (IC 50 < 10 μM) ( Table 3 ) [ 80 ].…”

Section: Natural Bioactive Inhibitors Of Oat3mentioning

confidence: 99%

Recent Advances in Synthetic Drugs and Natural Actives Interacting with OAT3

Cao

Wang

et al. 2023

Molecules

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Organic anion transporter 3 (OAT3) is predominantly expressed in the kidney and plays a vital role in drug clearance. Consequently, co-ingestion of two OAT3 substrates may alter the pharmacokinetics of the substrate. This review summarizes drug–drug interactions (DDIs) and herbal–drug interactions (HDIs) mediated by OAT3, and inhibitors of OAT3 in natural active compounds in the past decade. This provides a valuable reference for the combined use of substrate drugs/herbs for OAT3 in clinical practice in the future and for the screening of OAT3 inhibitors to avoid harmful interactions.

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Caffeic acid inhibits organic anion transporters OAT1 and OAT3 in rat kidney

Abstract: These findings show that caffeic acid inhibits OAT1 and OAT3 in the rat kidney.

Cited by 9 publications

References 14 publications

(−)-Epigallocatechin-3-gallate Inhibits Human and Rat Renal Organic Anion Transporters

(−)-Epigallocatechin-3-gallate Inhibits Human and Rat Renal Organic Anion Transporters

Metabolomics study of fasudil on cisplatin-induced kidney injury

Recent Advances in Synthetic Drugs and Natural Actives Interacting with OAT3

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