Cafeteria in extreme environments: Investigations on C. burkhardae and three new species from the Atacama Desert and the deep ocean

Schoenle, Alexandra; Hohlfeld, Manon; Rybarski, Alexandra; Sachs, Maria; Freches, Eric; Wiechmann, Karla; Nitsche, Frank; Arndt, Hartmut

doi:10.1016/j.ejop.2022.125905

Cited by 7 publications

(6 citation statements)

References 45 publications

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“…An exception to this observation was ASVs assigned to the genus Cafeteria (Bicoecea), which had high read abundances at all depths. Species belonging to the genus Cafeteria are known to be highly common and ubiquitously distributed in the world’s oceans and adapted to various habitat types, including the deep sea [ 49 , 50 ]. They attach with their posterior flagella to any surface.…”

Section: Resultsmentioning

confidence: 99%

“…The samples analyzed in this study were also used for a cultivation-based approach [ 15 ], resulting in several monoclonal cultures belonging mainly to Cafeteriaceae [ 50 ] and Percolomonadida [ 63 ]. We used these groups as model groups to check whether we can find V9 sequences belonging to our cultivated strains in the metabarcoding dataset.…”

Section: Resultsmentioning

confidence: 99%

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Distribution Patterns of Benthic Protist Communities Depending on Depth Revealed by Environmental Sequencing—From the Sublittoral to the Deep Sea

Hohlfeld

Arndt

2023

Microorganisms

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Protists are key components of the microbial food web in marine pelagic systems because they link algal and bacterial production to higher trophic levels. However, their functioning and bathymetric distribution in benthic deep-sea ecosystems are still only poorly understood. However, biogeographical patterns of communities can be coupled to the functioning of ecosystems and are therefore important to understand ecological and evolutionary processes. In this study, we investigated the diversity and distribution of benthic protist communities from the sublittoral down to the deep seafloor (50–2000 m) around three islands of the Azores in the North Atlantic Ocean. Using amplicon sequencing of the V9 region (18S rDNA) of 21 samples, we found that protist community compositions from different depths were significantly different. Three assemblages were separated along the following depths: 50 m, 150–500 m and 1000–2000 m, which indicate that deep-sea areas surrounding islands might act as isolating barriers for benthic protist species. A limited gene flow between the communities could favor speciation processes, leading to the unique protist communities found at the different investigated islands.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Distribution Patterns of Benthic Protist Communities Depending on Depth Revealed by Environmental Sequencing—From the Sublittoral to the Deep Sea

Hohlfeld

Arndt

2023

Microorganisms

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“…Other remarkable protist lineages are those taxa detected in Quepiaco Salar, represented by Cafeteria, Isochrysis, and Hartmannella, of which the first two include marine strains (Bendif et al, 2013;Schoenle et al, 2020). The occurrence of the genus Cafeteria has already been reported from the Atacama Desert (Schoenle et al, 2022).…”

Section: Alpha Diversity and Taxonomy Of Protists Across Five Microbi...mentioning

confidence: 96%

“…Differences in the salinity of the water bodies originated from different catchment areas (Ritter et al, 2018), explaining in part the uniqueness of environmental conditions in each salar/lagoon and the differences in community structure (Figure 6). The salinity is specifically tolerated by protist species from various phylogenetic lineages isolated from hypersaline lagoons and salars of the Atacama (Schiwitza et al, 2018(Schiwitza et al, , 2019(Schiwitza et al, , 2021Carduck et al, 2021;Rybarski et al, 2021;Schoenle et al, 2022).…”

Section: Factors Associated With the Benthic Protistsmentioning

confidence: 99%

“…), in saline microbial mats and hypersaline lagoons harboring biosignatures classified as diatoms, bicosoecids, and ciliates as detected by metabarcoding (Saghaï et al, 2017;Rybarski et al, 2023). Efforts to isolate and sequence the cultivable and uncultivable protist diversity from saline to hypersaline lagoons unveiled a novel endemic diversity including species from Opisthokonta (choanoflagellates), Rhizaria (Cercomonadida), Discoba (percolomonads), Stramenopiles (bicosoecids and placidids), and Alveolata (ciliates) (Schiwitza et al, 2018(Schiwitza et al, , 2019(Schiwitza et al, , 2021Arndt et al, 2020;Carduck et al, 2021;Rybarski et al, 2021Rybarski et al, , 2023Schoenle et al, 2022;Acosta et al, 2023;Hohlfeld et al, 2023).…”

Section: Introductionmentioning

confidence: 99%

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Protist communities of microbial mats from the extreme environments of five saline Andean lagoons at high altitudes in the Atacama Desert

Acosta,

Nitsche,

Dorador

et al. 2024

Front. Microbiol.

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IntroductionHeterotrophic protists colonizing microbial mats have received little attention over the last few years, despite their importance in microbial food webs. A significant challenge originates from the fact that many protists remain uncultivable and their functions remain poorly understood.MethodsMetabarcoding studies of protists in microbial mats across high-altitude lagoons of different salinities (4.3–34 practical salinity units) were carried out to provide insights into their vertical stratification at the millimeter scale. DNA and cDNA were analyzed for selected stations.ResultsSequence variants classified as the amoeboid rhizarian Rhogostoma and the ciliate Euplotes were found to be common members of the heterotrophic protist communities. They were accompanied by diatoms and kinetoplastids. Correlation analyses point to the salinity of the water column as a main driver influencing the structure of the protist communities at the five studied microbial mats. The active part of the protist communities was detected to be higher at lower salinities (<20 practical salinity units).DiscussionWe found a restricted overlap of the protist community between the different microbial mats indicating the uniqueness of these different aquatic habitats. On the other hand, the dominating genotypes present in metabarcoding were similar and could be isolated and sequenced in comparative studies (Rhogostoma, Euplotes, Neobodo). Our results provide a snapshot of the unculturable protist diversity thriving the benthic zone of five athalossohaline lagoons across the Andean plateau.

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High diversity and isolated distribution of aquatic heterotrophic protists in salars of the Atacama Desert at different salinities

Rybarski

Nitsche

Schoenle

et al. 2023

European Journal of Protistology

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Cafeteria in extreme environments: Investigations on C. burkhardae and three new species from the Atacama Desert and the deep ocean

Cited by 7 publications

References 45 publications

Distribution Patterns of Benthic Protist Communities Depending on Depth Revealed by Environmental Sequencing—From the Sublittoral to the Deep Sea

Distribution Patterns of Benthic Protist Communities Depending on Depth Revealed by Environmental Sequencing—From the Sublittoral to the Deep Sea

Protist communities of microbial mats from the extreme environments of five saline Andean lagoons at high altitudes in the Atacama Desert

High diversity and isolated distribution of aquatic heterotrophic protists in salars of the Atacama Desert at different salinities

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