Caenorhabditis elegans: a model to understand host–microbe interactions

Kumar, Arun; Baruah, Aiswarya; Tomioka, Masahiro; Iino, Yuichi; Kalita, Mohan Chandra; Khan, Mojibur R.

doi:10.1007/s00018-019-03319-7

Cited by 65 publications

(58 citation statements)

References 201 publications

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“…Previous reports elucidated how bacterial diet affects C. elegans as nutritional components, gut microbiota, or pathogen (Kumar et al, 2019; J. J. Zhou, Chun, & Liu, 2019).…”

Section: Discussionmentioning

confidence: 99%

Diets affect the age-dependent decline of associative learning in Caenorhabditis elegans

Higurashi

Tsukada

Nakano

et al. 2020

Preprint

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Diet is proposed to affect brain aging. However, the causality and mechanism of dietary effects on brain aging are still unclear due to the long time scales of aging. The nematode Caenorhabditis elegans (C. elegans) has led aging research because of its short lifespan and easy genetic manipulation. When fed the standard laboratory diet, Escherichia coli (E. coli), C. elegans experiences an age-dependent decline in temperature-food associative learning, called thermotaxis. To address if diet ameliorates this decline, we screened 35 different lactic acid bacteria as alternative diets. We found that Lactobacilli in a clade enriched with heterofermentative bacteria ameliorated age-dependent decline. On the other hand, homofermentative Lactobacillus species did not show this beneficial effect. Lactobacilli affected the thermotaxis of aged animals through DAF-16, an ortholog of mammalian FOXO transcription factor, while the effect on the thermotaxis was independent of the lifespan and locomotion. Our results demonstrate that diet can impact brain aging without changing the lifespan and that bacterial screen using C. elegans is a powerful approach to investigate age-dependent behavioral decline.

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“…Previous reports elucidated how bacterial diet affects C. elegans as nutritional components, gut microbiota, or pathogen (Kumar et al, 2019; J. J. Zhou, Chun, & Liu, 2019).…”

Section: Discussionmentioning

confidence: 99%

Diets affect the age-dependent decline of associative learning in Caenorhabditis elegans

Higurashi

Tsukada

Nakano

et al. 2020

Preprint

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“…A strong decrease of these parameters was observed in the isolate-fed nematodes. A shortened lifespan, a delayed larval development, and a limited pharyngeal pumping rate with a lowered body bending were all evident signs of a bacterial infection, proving that these two isolates have pathogenic behaviors [39,68].…”

Section: In Vivo Assaymentioning

confidence: 99%

“…Moreover, the possible pathogenicity of the two isolated bacteria was tested by using the Caenorhabditis elegans model system, a powerful in vivo tool for studying host-pathogen interactions. Its vitality, larval development, progeny, and neuro-muscular behaviors represent pathogenicity markers [39].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Bioaerosol Bacterial Components of a Wastewater Treatment Plant Through an Integrate Approach and In Vivo Assessment

Bruni

Simonetti

Bovone

et al. 2019

IJERPH

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Wastewater carries different pathogenic and non-pathogenic microorganisms that can be dispersed in the surrounding environment. Workers who frequent sewage treatment plants can therefore be exposed to aerosols that contain a high concentration of potentially dangerous biological agents, or they can come into direct contact with contaminated material. This can lead to allergies, infections and occupational health-associated diseases. A characterization of biological risk assessment of bioaerosol exposure is necessary. The aim of this study was to evaluate the application of an interdisciplinary method that combines chemical and biological approaches for the analysis of a bioaerosol derived from a wastewater treatment plant (WWTP) situated in Italy. Sampled filters were analyzed by HPLC-MS/MS spectroscopy that searched for different chemical biomarkers of airborne microorganisms. The analytical quantification was compared to the biological cultural method that revealed an underrated microbial concentration. Furthermore, next generation sequencing analysis was used also to identify the uncultivable species that were not detected by the culture dependent-method. Moreover, the simple animal model Caenorhabditis elegans was used to evaluate the pathogenicity of two isolates—Acinetobacter iwoffii and Micrococcus luteus—that showed multidrug-resistance. This work represents a starting point for the development of a multidisciplinary approach for the validation of bioaerosol exposure on WWTP workplaces.

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“…Invertebrate organisms such as the fruit y D. melanogaster and the nematode C. elegans with a relative short lifespan, availability of different genetic mutants and morphological and functional similarities of gut were broadly used as proof-of-concept models on microbiome-aging studies [18][19][20] . Some bacterial strains, such as Comamonas DA1877 and Lactobacillus gasseri SBT2055, have been identi ed to in uence the lifespan and reproduction of C. elegans by regulating series of signaling pathways [21][22][23] .…”

Section: B Adolescentis Supplement Improved Lifespan and Healthspanmentioning

confidence: 99%

Bifidobacterium adolescentis improves lifespan and healthspan by regulating catalase activity and oxidative stress-associated metabolites

Chen¹,

Chen²,

Qi³

et al. 2020

Preprint

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Microbiota-host interaction was involved in aging, while the specific bacterium was undetermined. To identify candidate bacterium with aging, we performed fecal microbiota sequencing. Less richness of gut microbial community, and a reduction of B.adolescentis abundance was observed in elderly individuals. B. adolescentis supplement improved osteoporosis and neurodegeneration in telomerase RNA component deletion (Terc−/−) aged mice. B.adolescentis induced prolongevity and healthspan improvement in Drosophila melanogaster and C. elegans. Transgenic deletion of ctl-2 in C. elegans abolished the effect on lifespan and healthspan by B. adolescentis. The catalase activity was decreased in skeletal muscle and brain tissues of Terc−/− mice, as well as cellular senescence in mouse embryonic fibroblasts. B. adolescentis alleviated ROS accumulation by regulation of oxidative stress-associated metabolites. These results suggest a role for B. adolescentis in improving lifespan and healthspan by regulating catalase activity and host metabolism. Supplement with commensal bacteria is a promising strategy against age related diseases.

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Caenorhabditis elegans: a model to understand host–microbe interactions

Cited by 65 publications

References 201 publications

Diets affect the age-dependent decline of associative learning in Caenorhabditis elegans

Diets affect the age-dependent decline of associative learning in Caenorhabditis elegans

Evaluation of Bioaerosol Bacterial Components of a Wastewater Treatment Plant Through an Integrate Approach and In Vivo Assessment

Bifidobacterium adolescentis improves lifespan and healthspan by regulating catalase activity and oxidative stress-associated metabolites

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