Caenorhabditis briggsae methods

Baird, Scott Everet; Chamberlin, Helen

doi:10.1895/wormbook.1.128.1

Cited by 15 publications

(13 citation statements)

References 30 publications

(15 reference statements)

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“…By identifying substantially greater genetic differentiation in the wild than previously recognized, as well as testing for heritable phenotypic variation, we have expanded the potential of Caenorhabditis briggsae as an emerging model for studies in molecular population genetics, quantitative genetics, the evolution of development and ecological genetics (Wang et al 2004;Baird et al 2005;Baird & Chamberlin 2006;Hill et al 2006;Hillier et al 2007;Dolgin et al 2008). Although we do not detect strong patterns of heritable phenotypic variation in the sensitivity of C. briggsae to a potentially ecologically important toxin -ethanol -our quantification of the norms of reaction in survival and reproduction nevertheless provides important new data about fitness-related traits in this organism.…”

Section: Discussionmentioning

confidence: 99%

Molecular population genetics and phenotypic sensitivity to ethanol for a globally diverse sample of the nematodeCaenorhabditis briggsae

Cutter¹,

Yan²,

Tsvetkov³

et al. 2010

Molecular Ecology

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New genomic resources and genetic tools of the past few years have advanced the nematode genus Caenorhabditis as a model for comparative biology. However, understanding of natural genetic variation at molecular and phenotypic levels remains rudimentary for most species in this genus, and for C. briggsae in particular. Here we characterize phenotypic variation in C. briggsae's sensitivity to the potentially important and variable environmental toxin, ethanol, for globally diverse strains. We also quantify nucleotide variation in a new sample of 32 strains from four continents, including small islands, and for the closest-known relative of this species (C. sp. 9). We demonstrate that C. briggsae exhibits little heritable variation for the effects of ethanol on the norm of reaction for survival and reproduction. Moreover, C. briggsae does not differ significantly from C. elegans in our assays of its response to this substance that both species likely encounter regularly in habitats of rotting fruit and vegetation. However, we uncover drastically more molecular genetic variation than was known previously for this species, despite most strains, including all island strains, conforming to the broad biogeographic patterns described previously. Using patterns of sequence divergence between populations and between species, we estimate that the self-fertilizing mode of reproduction by hermaphrodites in C. briggsae likely evolved sometime between 0.9 and 10 million generations ago. These insights into C. briggsae's natural history and natural genetic variation greatly expand the potential of this organism as an emerging model for studies in molecular and quantitative genetics, the evolution of development, and ecological genetics.

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Section: Discussionmentioning

confidence: 99%

Molecular population genetics and phenotypic sensitivity to ethanol for a globally diverse sample of the nematodeCaenorhabditis briggsae

Cutter¹,

Yan²,

Tsvetkov³

et al. 2010

Molecular Ecology

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“…The biogeographic patterning of C. briggsae could make this species more amenable than C. elegans to studies of ecological and behavioral adaptation (Baird and Chamberlin, 2006;Cutter et al, 2006b). The few studies that examined strain-specific differences in C. briggsae have shown that strains from different clades exhibit variation in hybrid compatibilities (Baird, 2002), the patterning of sensory rays in the male tail (Baird, 2001) and vulval cell lineages (Delattre and Félix, 2001).…”

Section: Diversity In African Caenorhabditismentioning

confidence: 99%

“…Despite analogous breeding systems and similar morphology (Nigon and Dougherty, 1949), their genome sequences have diverged drastically (Coghlan and Wolfe, 2002;Stein et al, 2003), although they do show a high degree of chromosomal synteny (Hillier et al, 2007). The development of C. briggsae as a laboratory system is still in its infancy, but with a growing number of genetic resources becoming available, including a sequenced genome and recombination maps, this species is emerging as a useful companion species of C. elegans (Baird and Chamberlin, 2006).…”

Section: Introductionmentioning

confidence: 99%

Hakuna Nematoda: genetic and phenotypic diversity in African isolates of Caenorhabditis elegans and C. briggsae

Cutter

2007

Heredity

101

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“…Recent estimates for the divergence time of the two species range from as low as 4–30 MYA to as high as 80–110 MYA (Cutter, 2008; Stein et al, 2003). Similar experimental tools, including a sequenced genome and ability to perform RNAi, are available in both species (Baird and Chamberlin, 2006). In this study, we examine the maternal specification of two embryonic precursors, MS and E.…”

Section: Introductionmentioning

confidence: 99%

Knockdown of SKN-1 and the Wnt effector TCF/POP-1 reveals differences in endomesoderm specification in C. briggsae as compared with C. elegans

Lin

Broitman-Maduro

Hung

et al. 2009

Developmental Biology

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In the nematode, C. elegans, the bZIP/homeodomain transcription factor SKN-1 and the Wnt effector TCF/POP-1 are central to the maternal specification of the endomesoderm prior to gastrulation. The 8-cell stage blastomere MS is primarily a mesodermal precursor, giving rise to cells of the pharynx and body muscle among others, while its sister E clonally generates the entire endoderm (gut). In C. elegans, loss of SKN-1 results in the absence of MS-derived tissues all of the time, and loss of gut most of the time, while loss of POP-1 results in a mis-specification of MS as an E-like cell, resulting in ectopic gut. We show that in C. briggsae, RNAi of skn-1 results in a stronger E defect but no apparent MS defect, while RNAi of pop-1 results in loss of gut and an apparent E to MS transformation, the opposite of the pop-1 knockdown phenotype seen in C. elegans. The difference in pop-1(−) phenotypes correlates with changes in how the endogenous endoderm-specifying end genes are regulated by POP-1 in the two species. Our results suggest that integration of Wnt-dependent and Wnt-independent cell fate specification pathways within the Caenorhabditis genus can occur in different ways.

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Caenorhabditis briggsae methods

Cited by 15 publications

References 30 publications

Molecular population genetics and phenotypic sensitivity to ethanol for a globally diverse sample of the nematodeCaenorhabditis briggsae

Molecular population genetics and phenotypic sensitivity to ethanol for a globally diverse sample of the nematodeCaenorhabditis briggsae

Hakuna Nematoda: genetic and phenotypic diversity in African isolates of Caenorhabditis elegans and C. briggsae

Knockdown of SKN-1 and the Wnt effector TCF/POP-1 reveals differences in endomesoderm specification in C. briggsae as compared with C. elegans

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