Cadophora finlandia and Phialocephala fortinii: Agrobacterium-mediated transformation and functional GFP expression

Gorfer, Markus; Klaubauf, Sylvia; Bandian, Dragana; Strauss, Joseph

doi:10.1016/j.mycres.2007.05.002

Cited by 24 publications

(16 citation statements)

References 22 publications

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“…aleophilum conidial suspension according to Gorfer et al . [25]. The binary vector pCBCT contains gfp under the control of the strong toxA promoter [26] and the hygromycine resistance marker hph .…”

Section: Methodsmentioning

confidence: 99%

Deciphering the Niches of Colonisation of Vitis vinifera L. by the Esca-Associated Fungus Phaeoacremonium aleophilum Using a gfp Marked Strain and Cutting Systems

et al. 2015

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IntroductionEsca disease has become a major threat for viticulture. Phaeoacremonium aleophilum is considered a pioneer of the esca complex pathosystem, but its colonisation behaviour inside plants remains poorly investigated.Material and MethodsIn this study, P. aleophilum::gfp7 colonisation was assessed six and twelve weeks post-inoculation in two different types of tissues: in the node and the internode of one year-old rooted cuttings of Cabernet Sauvignon. These processes of colonisation were compared with the colonisation by the wild-type strain using a non-specific lectin probe Alexa Fluor 488-WGA.ResultsData showed that six weeks post-inoculation of the internode, the fungus had colonised the inoculation point, the bark and xylem fibres. Bark, pith and xylem fibres were strongly colonised by the fungus twelve weeks post-inoculation and it can progress up to 8 mm from the point of inoculation using pith, bark and fibres. P. aleophilum was additionally detected in the lumen of xylem vessels in which tyloses blocked its progression. Different plant responses in specific tissues were additionally visualised. Inoculation of nodes led to restricted colonisation of P. aleophilum and this colonisation was associated with a plant response six weeks post-inoculation. The fungus was however detected in xylem vessels, bark and inside the pith twelve weeks post-inoculation.ConclusionsThese results demonstrate that P. aleophilum colonisation can vary according to the type of tissues and the type of spread using pith, bark and fibres. Woody tissues can respond to the injury and to the presence of this fungus, and xylem fibres play a key role in the early colonisation of the internode by P. aleophilum before the fungus can colonise xylem vessels.

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Section: Methodsmentioning

confidence: 99%

Deciphering the Niches of Colonisation of Vitis vinifera L. by the Esca-Associated Fungus Phaeoacremonium aleophilum Using a gfp Marked Strain and Cutting Systems

et al. 2015

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“…Melting curve analysis was done in order to confirm the specificity of the PCR product. As standard pure culture, genomic DNA from Cadophora finlandica PRF15 (Gorfer et al 2007) was used as reference. To produce the quantitative data for fungal and total DNA (for Fig.…”

Section: Methodsmentioning

confidence: 99%

Plants control the seasonal dynamics of microbial N cycling in a beech forest soil by belowground C allocation

Kaiser

Fuchslueger

Koranda

et al. 2011

Ecology

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Soil microbes in temperate forest ecosystems are able to cycle several hundreds of kilograms of N per hectare per year and are therefore of paramount importance for N retention. Belowground C allocation by trees is an important driver of seasonal microbial dynamics and may thus directly affect N transformation processes over the course of the year. Our study aimed at unraveling plant controls on soil N cycling in a temperate beech forest at a high temporal resolution over a time period of two years, by investigating the effects of tree girdling on microbial N turnover. In both years of the experiment, we discovered (1) a summer N mineralization phase (between July and August) and (2) a winter N immobilization phase (November-February). The summer mineralization phase was characterized by a high N mineralization activity, low microbial N uptake, and a subsequent high N availability in the soil. During the autumn/winter N immobilization phase, gross N mineralization rates were low, and microbial N uptake exceeded microbial N mineralization, which led to high levels of N in the microbial biomass and low N availability in the soil. The observed immobilization phase during the winter may play a crucial role for ecosystem functioning, since it could protect dissolved N that is produced by autumn litter degradation from being lost from the ecosystem during the phase when plants are mostly inactive. The difference between microbial biomass N levels in winter and spring equals 38 kg N/ha and may thus account for almost one-third of the annual plant N demand. Tree girdling strongly affected annual N cycling: the winter N immobilization phase disappeared in girdled plots (microbial N uptake and microbial biomass N were significantly reduced, while the amount of available N in the soil solution was enhanced). This was correlated to a reduced fungal abundance in autumn in girdled plots. By releasing recently fixed photosynthates to the soil, plants may thus actively control the annual microbial N cycle. Tree belowground C allocation increases N accumulation in microorganisms during the winter which may ultimately feed back on plant N availability in the following growing season.

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“…Experiments similar to those with P. indica and the green fl uorescent protein (GFP)-tagged Arabidopsis used by Jacobs et al ( 2011 ) and Qiang et al ( 2012b ) will likely prove invaluable in following the DSE colonization. Alternatively, GFP-tagged DSE fungi can provide a mycocentric view of the colonization, for example (Gorfer et al 2007 ), GFPtagged Cadophora fi nlandica and P . fortinii that were stable in repeated subculturing.…”

Section: Evaluation Of Dse Function Using Arabidopsis Hostmentioning

confidence: 99%

Unraveling the Dark Septate Endophyte Functions: Insights from the Arabidopsis Model

Mandyam

Jumpponen

2013

Advances in Endophytic Research

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The global occurrence of plant root-associated fungal endophytes and their great abundance in many habitats necessitate studies to decipher their potential functions. Improved understanding of the basic endophyte ecology including host range, host preference, and host responses to endophyte colonization has been made possible through populations of endophytes (e.g., Periconia macrospinosa and Microdochium sp.) isolated from North American native tallgrass prairie. The recent demonstration of the endophyte symbiosis of the model plant Arabidopsis thaliana has provided additional tools to further elucidate the ecology of these endophytes. The availability of a large number of Arabidopsis ecotypes and mutants, microarrays, and databases allows the molecular dissection of endophyte symbiosis to better understand the importance of fungal endophytes in host nutrient uptake, defenses, and/or responses to pathogens and stress. In this chapter, we discuss the ecology and functions of endophytic fungi through experiments utilizing the Arabidopsis model system. We draw parallels with another deeply dissected Piriformospora indica root endophyte symbiosis, which has been demonstrated to promote growth of model and non-model plants.

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Cadophora finlandia and Phialocephala fortinii: Agrobacterium-mediated transformation and functional GFP expression

Cited by 24 publications

References 22 publications

Deciphering the Niches of Colonisation of Vitis vinifera L. by the Esca-Associated Fungus Phaeoacremonium aleophilum Using a gfp Marked Strain and Cutting Systems

Deciphering the Niches of Colonisation of Vitis vinifera L. by the Esca-Associated Fungus Phaeoacremonium aleophilum Using a gfp Marked Strain and Cutting Systems

Plants control the seasonal dynamics of microbial N cycling in a beech forest soil by belowground C allocation

Unraveling the Dark Septate Endophyte Functions: Insights from the Arabidopsis Model

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