Cadmium‐tolerant bacteria: current trends and applications in agriculture

Bravo, Daniel; Braissant, Olivier

doi:10.1111/lam.13594

Cited by 27 publications

(11 citation statements)

References 162 publications

(246 reference statements)

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“…The amplification of the smt and CadA genes is of note because they are related to metabolic pathways where the energy activation (ATPase systems) is needed, which could be expressed by the activation of the cad operon. The P-type ATPase efflux system causes transport of the metal by ATP hydrolysis, so the reaction is considered endothermic, due to the need for energy to hydrolyse ATP (Bravo and Braissant, 2022). This implies that the presence of these genes from CdtB populations in the assessed soil samples requires energy, which is confirmed by the endothermic reactions observed in Figure 2, and the last values of Figure 3, particularly when Cd is amended.…”

Section: Immobilization Capacity Of Cdtb In Soilmentioning

confidence: 68%

“…Even if the amplification does not mean that all the populations that have these genes are active, the brightness and width of the bands indicate a good concentration of the genes within the whole community of the composite soil samples. CadA gene is implied in cation pump exchange at periplasmic level, where internalization through the bioweathering pathway is the first step towards biological induction of CdCO 3 or secondary otavite (Bravo and Braissant, 2022). Likewise, smt gene is involved in the regulation of ATPase dependant on reduction of Cd from soluble forms such as chlorates, or sulphates present in soil that CdtB transforms into non-soluble and geostable forms.…”

Section: Immobilization Capacity Of Cdtb In Soilmentioning

confidence: 99%

“…CdtB are a non-phylogenetically related group of bacteria with the exceptional capacity of metabolize Cd and using it as source of energy (Bravo and Braissant, 2022). Their metabolic capacity may vary depending on the soil type, the soil Cd content as well as other soil characteristics (Bravo et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

“…Besides, the amplification of genes related to Cd immobilization is a first step to know if CdtB population are present in the bacterial community. The CadA and smt genes have been highlighted as an important resource to estimate the presence of CdtB (Bravo and Braissant, 2022).…”

Section: Introductionmentioning

confidence: 99%

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Bacterial Cadmium-Immobilization Activity Measured by Isothermal Microcalorimetry in Cacao-Growing Soils From Colombia

Bravo

2022

Front. Environ. Sci.

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In cacao farms, the presence of cadmium (Cd) is a major issue for commercialization, particularly for countries such as Colombia. Cadmium-tolerant bacteria (CdtB) are an important functional group of microorganisms with a potential for bioremediation strategies. Cd immobilization activity by CdtB can be accurately measured by isothermal microcalorimetry (IMC). In this study, the metabolic capacity of an entire CdtB community in cacao farm soils from three cacao-producing districts of Colombia, with and without the addition of Cd was measured using IMC. The differences between the observed peaks in metabolic activity related to Cd immobilization were analysed to determine whether activation of CdtB populations occurred when Cd content was increased. The thermograms from Santander soil samples have a major metabolic activity of the CdtB community compared to peaks of maximal heat-flow in the samples from Antioquia and Arauca. IMC showed differences in Cd immobilization ratios of the soil samples of 0.11–0.30 mg kg−1 h−1 at 25°C over 12 days of thermal monitoring. Furthermore, the amplicons of cadA and smt genes from the soil samples allow elucidation of possible metabolic mechanisms used by CdtB soil populations. The gene amplification confirmed the existence of CdtB populations related to both bioweathering and biochelating metabolic capacities. Scanning electron microscope (SEM) images supported the existence of otavite biologically induced by CdtB naturally. A Pearson correlation analysis was made between kinetical growth parameters and thermodynamic data. Besides, a PCA was performed between CdtB cadA gene copies, soil pH and SOM indicating the effect of CdtB in Cd translocation. Thus, it is concluded that the combination of Cd immobilization ratios obtained using isothermal microcalorimetry, the molecular basis of metabolic immobilization, and SEM imagery could act as a useful toolkit to identify CdtB populations for bioremediation strategies in contaminated cacao farms. The research importance of this study is the use of combined tools for quantitative IMC measurements to identify and assess Cd metabolic capacities of CdtB populations in soil, in situ, as a new proxy for CdtB assessment in cacao-growing soils.

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Section: Immobilization Capacity Of Cdtb In Soilmentioning

confidence: 68%

Section: Immobilization Capacity Of Cdtb In Soilmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Bacterial Cadmium-Immobilization Activity Measured by Isothermal Microcalorimetry in Cacao-Growing Soils From Colombia

Bravo

2022

Front. Environ. Sci.

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“…The use of microorganisms with the ability to adsorb, bioaccumulate and biotransform Cd is an attractive approach for carrying out bioremediation processes of contaminated soils (Ashraf et al 2017). Soil bacteria have several biochemical tolerance mechanisms to overcome toxic levels of Cd such as biosorption, intracellular sequestration (bioaccumulation), extracellular binding (bioleaching), complexation (biotransformation), biodegradation, chemisorption, and bioweathering (Bravo and Braissant 2021). A reduction of Cd uptake by plants using Cd tolerant bacteria (CdtB) and Cd tolerant plant growth-promoting rhizobacteria (PGPR) has been reported in wheat and maize (Ahmad et al 2014), rice (Treesubsuntorn et al 2017), mustard (Sinha and Mukherjee 2008), tomato (Madhaiyan et al 2007), pea (Safronova et al 2006), and soybean (Guo and Chi 2014).…”

Section: Introductionmentioning

confidence: 99%

Assessment of native cadmium-tolerant bacteria in cacao (Theobroma cacao L.) - cultivated soils in Cundinamarca-Colombia

Cordoba-Novoa

Cáceres-Zambrano

Torres‐Rojas

2022

Preprint

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Traces of cadmium (Cd) have been reported in chocolate products due to growing cacao plants in soils with Cd and the ability of cacao plants to accumulate it. A strategy to minimize this uptake of Cd is by using cadmium-tolerant bacteria (CdtB) to sequester the Cd. This study was aimed to isolate and characterize CdtB associated with cacao-cultivated soils in Cundinamarca-Colombia. Thirty CdtB morphotypes were isolated and almost half of them showed the capacity to fix nitrogen, solubilize phosphates and degrade cellulose. Besides, all strains were selected for analysis of 16S rRNA sequence. The soil with the highest natural concentration of Cd (location Y3) showed higher abundance, unique morphotypes, and dominance of isolated CdtB. Pseudomona putida GB78, Pseundomona aeruginosa NB2, and Burkholderia sp. NB10 were the only morphotypes that grew on 18 up to 90 (GB78) and 140 mg Kg− 1 Cd (NB2-NB10). GB78 showed the highest Cd bioaccumulation (5.92 mg g− 1). Our results expand the knowledge about culturable CdtB in cacao-cultivated soils with the potential to develop soil management strategies to mitigate the uptake of Cd by cacao plants, thereby preventing the potential impact on human health, food safety, and the cacao economy.

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