Cadmium Sulfide Nanoparticle Synthesis in Dps Protein from <i>Listeria </i><i>innocua</i>

Iwahori, Keisuke; Enomoto, Takahiro; Furusho, Hirotoshi; Miura, Atsushi; Nishio, Kazuaki; Mishima, Yumiko; Yamashita, Ichiro

doi:10.1021/cm0628799

Cited by 66 publications

(68 citation statements)

References 25 publications

(31 reference statements)

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“…For example, peptides displayed on the surface of bacteriophage have been used to produce intricately ordered CdS and ZnS nanowires (24), whereas engineered viral particles (25,26) along with recombinant, self-assembled cage-like proteins (27) have served as nucleation cavities for CdS nanocrystals. Spoerke and Voigt (21) engineered a library of CdS-capping peptides capable of templating CdS nanocrystal growth from cadmium acetate and Na 2 S, with the resulting optical properties dependent on the chemical structure of the capping peptide.…”

mentioning

confidence: 99%

Single-enzyme biomineralization of cadmium sulfide nanocrystals with controlled optical properties

Dunleavy

Kiely

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

115

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Nature has evolved several unique biomineralization strategies to direct the synthesis and growth of inorganic materials. These natural systems are complex, involving the interaction of multiple biomolecules to catalyze biomineralization and template growth. Herein we describe the first report to our knowledge of a single enzyme capable of both catalyzing mineralization in otherwise unreactive solution and of templating nanocrystal growth. A recombinant putative cystathionine γ-lyase (smCSE) mineralizes CdS from an aqueous cadmium acetate solution via reactive H 2 S generation from L-cysteine and controls nanocrystal growth within the quantum confined size range. The role of enzymatic nanocrystal templating is demonstrated by substituting reactive Na 2 S as the sulfur source. Whereas bulk CdS is formed in the absence of the enzyme or other capping agents, nanocrystal formation is observed when smCSE is present to control the growth. This dualfunction, single-enzyme, aerobic, and aqueous route to functional material synthesis demonstrates the powerful potential of engineered functional material biomineralization.cadmium sulfide | quantum dot | biomineralization | enzyme | nanoparticle B iological systems have evolved a diverse array of mechanisms to synthesize inorganic materials from aqueous solutions under ambient conditions. This inherent control over material properties has created interest in using these biological routes to synthesize materials (1-3) such as biosilica from sponges and diatoms (4-8), biogenic CaCO 3 from mollusks (9-12), and magnetic particles from magnetotactic bacteria (13-15). Designing a biomineralization strategy requires control of both the material composition and structure; in nature, this control is typically achieved through the assembly of a multiprotein complex, including both structuredirecting proteins and proteins responsible for mineralization of a specific composition. In the current work, we demonstrate the reduction of this complexity to its simplest form: a single enzyme capable of both catalyzing CdS mineralization and controlling particle size within the quantum confined size range to form functional biomineralized CdS quantum dots.Two of the most studied biomineralization proteins are perlucin and silicatein. Perlucin (16) has been shown to mineralize crystalline forms of CaCO 3 , a common structural material that constitutes the shell of many marine organisms, in the form of organic-inorganic composites. The role of the nacre protein perlucin in crystallite templating has been elucidated through experiments demonstrating crystallite formation in the presence of purified perlucin, and perlucin selectively being removed from solution during crystallite formation in the presence of a mixture of water-soluble, nacre-associated proteins (17). Native silicatein harvested from sea sponge or engineered forms produced recombinantly are active for biomineralization of silica and titania into structures that are amorphous or crystalline (7,18,19). In particular, biomineralizatio...

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mentioning

confidence: 99%

Single-enzyme biomineralization of cadmium sulfide nanocrystals with controlled optical properties

Dunleavy

Kiely

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

115

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“…Thus, the same strategies as for apoferritin have been employed, while the smaller size of LIDps should restrict NP diameters to less than 4.5 nm. For example, 5Fe 2 25,52) In the reverse case, almost no NPs form inside the LIDps. Again, the positively charged Cd 2+ enters the cavity easily, as in the case of apoferritin.…”

Section: Apoferritinmentioning

confidence: 99%

“…50,51) Similar to apoferritin, the Dps cavity has been used as a nanoscale chamber for NP formation. 25,52,53) The N-and C-termini, which are both positioned on its outer surface, have been used to introduce additional functionalities for device applications, 54) such as dye-sensitized solar cells. 55) 2.2 TMV TMV was one of the first isolated viruses and is one of the best-characterized plant viruses.…”

Section: Structures and Assemblymentioning

confidence: 99%

Nanoscale device architectures derived from biological assemblies: The case of tobacco mosaic virus and (apo)ferritin

Calò

Eiben

Okuda

et al. 2016

Jpn. J. Appl. Phys.

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Virus particles and proteins are excellent examples of naturally occurring structures with well-defined nanoscale architectures, for example, cages and tubes. These structures can be employed in a bottom-up assembly strategy to fabricate repetitive patterns of hybrid organic–inorganic materials. In this paper, we review methods of assembly that make use of protein and virus scaffolds to fabricate patterned nanostructures with very high spatial control. We chose (apo)ferritin and tobacco mosaic virus (TMV) as model examples that have already been applied successfully in nanobiotechnology. Their interior space and their exterior surfaces can be mineralized with inorganic layers or nanoparticles. Furthermore, their native assembly abilities can be exploited to generate periodic architectures for integration in electrical and magnetic devices. We introduce the state of the art and describe recent advances in biomineralization techniques, patterning and device production with (apo)ferritin and TMV.

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“…First, library amino acids and surfactants were randomly mixed in 48 wells of a multi-well plate, such that each well contained between 1-6 amino acids and 1-3 surfactants (Figure 1 A). Cadmium chloride and thioacetic acid (as a sulfur source) [ 20 ] were then added to a concentration of 1 × 10 −3 M in all wells, as precursors for CdS. After 3 d the plate was viewed under UV illumination (Figure 1 A) and with a fl uorimetric Biomineralization, which is the process by which biological organisms form ceramic-based composites, has been widely used as an inspiration for the development of new strategies for materials synthesis.…”

Section: Doi: 101002/adma201403185mentioning

confidence: 99%

“…Quantum dot growth then progresses as sulfur is released from thioacetate degradation, where this takes place over several days. [ 20 ] Growth of the superstructures is then likely to occur by two mechanisms: accretion of the quantum dots onto the existing superstructure templates, and nucleation (and subsequent growth) of CdS quantum dots at the metal sites of the metal-organic scaffolds. This process is consistent with the observed red-shift in the UV-vis absorption (Figure 4 C), where this corresponds to the formation of larger quantum dots.…”

Section: Doi: 101002/adma201403185mentioning

confidence: 99%

Genetic Algorithm‐Guided Discovery of Additive Combinations That Direct Quantum Dot Assembly

et al. 2014

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these techniques are limited by the requirement for complex enzymatic reactions. As an alternative strategy that bypasses the need for genetic engineering, combinatorial methods can be employed. These can be used to explore tens to hundreds of reaction conditions, where the most promising or "lead" conditions may be selected based on the structures or properties of the resultant material. [ 17,18 ] Lead conditions can then be used to narrow the reaction landscape in successive screening rounds. Surprisingly, although combinatorial methods are often used in solid-state chemistry to explore, for example, different reagent compositions, [ 18 ] we are not aware of their use in identifying soluble additives capable of directing mineralization.In this article we demonstrate how combinatorial methods can be used in conjunction with effi cient screening processes to rapidly identify combinations of small organic molecules that are capable of directing the formation of photoluminescent quantum dot minerals in aqueous solution and at room temperature. Indeed, a key feature of biomineralization processes is that control over mineral formation is achieved using many soluble additives that operate in concert. That this feature has seldom been addressed in bioinspired methods is almost certainly due to the vast number of potential variables, which renders a full, systematic exploration intractable. As a solution to this challenge, we here utilize a genetic algorithm as a bioinspired heuristic that mimics natural evolution. Genetic algorithms use selection, recombination, and mutation strategies to rapidly identify and optimize the combination of conditions (here, soluble additives), which gives rise to materials with target properties. [ 19 ] Using a pipetting robot to prepare reaction sets and a UV-light table to rapidly assess the reactions for the formation of photoluminescent minerals, we are able to rapidly identify the key additives that promote the formation of quantum dot superstructures from one-pot aqueous reactions.Our initial library of organic mediators included 23 components, of which 17 were amino acids and 6 were surfactants. Stock solutions of the amino acids were prepared to initial concentrations of 100 × 10 −3 M , and explored at concentrations ranging from 0.01 to 50 × 10 −3 M , while surfactants were prepared to near their solubility limits in water. Surfactants were included as potential structure-directing agents to drive hierarchical assembly in aqueous solution. The overall screening approach used to identify the key additive set is summarized in Figure 1 . First, library amino acids and surfactants were randomly mixed in 48 wells of a multi-well plate, such that each well contained between 1-6 amino acids and 1-3 surfactants (Figure 1 A). Cadmium chloride and thioacetic acid (as a sulfur source) [ 20 ] were then added to a concentration of 1 × 10 −3 M in all wells, as precursors for CdS. After 3 d the plate was viewed under UV illumination (Figure 1 A) and with a fl uorimetric Biomineralization, whi...

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Cadmium Sulfide Nanoparticle Synthesis in Dps Protein from Listeria innocua

Cited by 66 publications

References 25 publications

Single-enzyme biomineralization of cadmium sulfide nanocrystals with controlled optical properties

Single-enzyme biomineralization of cadmium sulfide nanocrystals with controlled optical properties

Nanoscale device architectures derived from biological assemblies: The case of tobacco mosaic virus and (apo)ferritin

Genetic Algorithm‐Guided Discovery of Additive Combinations That Direct Quantum Dot Assembly

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