Cadmium sulfate induces apoptosis in planarians

Wang, Zhiyang; Cao, Keqing; Wang, Dan; Hua, Bingjie; Zhang, Haiyan; Xie, Xin

doi:10.1007/s11356-020-09991-8

Cited by 2 publications

(2 citation statements)

References 60 publications

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“…Correspondingly, the TUNEL‐positive cells we quantified corroborated the significant increase during MRLC RNAi knockdown (Figure 5B ). We quantified the expression levels of caspase‐2 , 48 caspase‐7 , 49 the pro‐apoptotic gene bak 50 and antiapoptotic gene Bcl‐2 , 51 which are reported to be involved in apoptosis. qPCR quantification showed their upregulation in MRLC RNAi animals ( n = 30 animals; Figure 5C ).…”

Section: Resultsmentioning

confidence: 99%

MRLC controls apoptotic cell death and functions to regulate epidermal development during planarian regeneration and homeostasis

et al. 2023

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Adult stem cells (ASCs) are pluripotent cells with the capacity to self‐renew and constantly replace lost cells due to physiological turnover or injury. Understanding the molecular mechanisms of the precise coordination of stem cell proliferation and proper cell fate decision is important to regeneration and organismal homeostasis. The planarian epidermis provides a highly tractable model to study ASC complex dynamic due to the distinct spatiotemporal differentiation stages during lineage development. Here, we identified the myosin regulatory light chain (MRLC) homologue in the Dugesia japonica transcriptome. We found high expression levels of MRLC in wound region during regeneration and also expressed in late epidermal progenitors as an essential regulator of the lineage from neoblasts to mature epidermal cells. We investigated the function of MRLC using in situ hybridization, real‐time polymerase chain reaction and double fluorescent and uncovered the potential mechanism. Knockdown of MRLC leads to a remarkable increase in cell death, causes severe abnormalities during regeneration and homeostasis and eventually leads to animal death. The global decrease in epidermal cell in MRLC RNAi animals induces accelerated epidermal proliferation and differentiation. Additionally, we find that MRLC is co‐expressed with cdc42 and acts cooperatively to control the epidermal lineage development by affecting cell death. Our results uncover an important role of MRLC, as an inhibitor of apoptosis, involves in epidermal development.

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Section: Resultsmentioning

confidence: 99%

MRLC controls apoptotic cell death and functions to regulate epidermal development during planarian regeneration and homeostasis

et al. 2023

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“…Early stages of carcinogenesis can be induced by exposing them to heavy metals or hydrocarbons [39][40][41]. Their DNA reparation pathways and tumour suppressor genes are activated by an exposure to carcinogens which increase the costs associated with these functions for the animals [39,42,43]. They are also ecologically relevant, as G. tigrina has a widespread distribution, including in Australia, and is an invasive species which feeds on native invertebrates [44].…”

Section: Introductionmentioning

confidence: 99%

Behavioural ecology meets oncology: quantifying the recovery of animal behaviour to a transient exposure to a cancer risk factor

Klaassen,

Tissot,

Meliani

et al. 2024

Proc. R. Soc. B.

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Wildlife is increasingly exposed to sublethal transient cancer risk factors, including mutagenic substances, which activates their anti-cancer defences, promotes tumourigenesis, and may negatively impact populations. Little is known about how exposure to cancer risk factors impacts the behaviour of wildlife. Here, we investigated the effects of a sublethal, short-term exposure to a carcinogen at environmentally relevant concentrations on the activity patterns of wild Girardia tigrina planaria during a two-phase experiment, consisting of a 7-day exposure to cadmium period followed by a 7-day recovery period. To comprehensively explore the effects of the exposure on activity patterns, we employed the double hierarchical generalized linear model framework which explicitly models residual intraindividual variability in addition to the mean and variance of the population. We found that exposed planaria were less active compared to unexposed individuals and were able to recover to pre-exposure activity levels albeit with a reduced variance in activity at the start of the recovery phase. Planaria showing high activity levels were less predictable with larger daily activity variations and higher residual variance. Thus, the shift in behavioural variability induced by an exposure to a cancer risk factor can be quantified using advanced tools from the field of behavioural ecology. This is required to understand how tumourous processes affect the ecology of species.

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Cadmium sulfate induces apoptosis in planarians

Cited by 2 publications

References 60 publications

MRLC controls apoptotic cell death and functions to regulate epidermal development during planarian regeneration and homeostasis

MRLC controls apoptotic cell death and functions to regulate epidermal development during planarian regeneration and homeostasis

Behavioural ecology meets oncology: quantifying the recovery of animal behaviour to a transient exposure to a cancer risk factor

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