Cadmium-stress in white lupin: effects on nodule structure and functioning

Carpena, Ramón O.; Vázquez, Saúl; Esteban, Elvira; Fernández‐Pascual, Mercedes; Felipe, María Rosario de; Zornoza, Pilar

doi:10.1016/s0981-9428(03)00136-0

Cited by 67 publications

(37 citation statements)

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“…Georgiev et al (1996) reported a lower total N content in the nodules and other plant organs of Robinia pseudoacacia subjected to Cu excess. A reduction in the amount of N 2 fixed and amino acids present in the nodules of white lupins exposed to Cd has also been reported (Carpena et al, 2003).…”

Section: Discussionmentioning

confidence: 76%

“…The adverse effect of abiotic stresses on N 2 -fixation involve the nodule cell colonisation process, as well as nodule growth, development and functioning (Carpena et al, 2003). Certainly, high levels of Cu reduce nodule number and weight in Vigna unguiculata (Kopittke et al, 2007) and Vigna radiata (Manivasagaperumal et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

“…The inhibition of nodulation and N 2 fixation can be caused by the accumulation of heavy metals in plant tissues, ultrastructural changes in nodule tissues (Carpena et al, 2003) and the negative effect of oxidative stress (Nasr-Esfahani et al, 2010). In the present work, LTSEM-EDXMA showed that Cu was mainly localised in the inner cortex and in the infected zone in white lupin nodules.…”

Section: Discussionmentioning

confidence: 99%

“…Soybean is another candidate, but few studies have examined N 2 fixation by the species in Cu-contaminated soils, despite it being the most economically important of all grain legumes. The present study compares the response of white lupin and soybean to Cu excess to determine: (1) whether nodulation and N 2 fixation are altered by the accumulation of Cu, ultrastructural changes caused by Cu, or by the appearance of oxidative stress, (2) whether nodules that accumulate Cu in the cell wall or vacuole show greater or lesser tolerance to Cu excess (it has been reported that Cd accumulation in nodule cell walls leads to greater Cd tolerance [Carpena et al, 2003]), and (3) whether the induction of the antioxidant response has a protective role under Cu excess, as reported for the nodules of plants grown under conditions of salinity and drought (Mhadhbi et al, 2004;Nasr-Esfahani et al, 2010).…”

mentioning

confidence: 99%

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Copper microlocalisation, ultrastructural alterations and antioxidant responses in the nodules of white lupin and soybean plants grown under conditions of copper excess

Sánchez-Pardo

Fernández‐Pascual

Zornoza

2012

Environmental and Experimental Botany

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Esta es la versión de autor del artículo publicado en:This is an author produced version of a paper published in: sensitive to Cu excess than those of soybean, with the nodulation process, N 2 fixation, and the ultrastructure of bacteroids more strongly affected. A less effective antioxidative stress response against Cu was also seen in white lupin than in soybean nodules: the excess copper treatment induced a smaller increase in the total thiol content and ascorbate peroxidase activity in white lupin nodules than in soybean nodules, and promoted a greater reduction in catalase activity.

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Section: Discussionmentioning

confidence: 76%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Copper microlocalisation, ultrastructural alterations and antioxidant responses in the nodules of white lupin and soybean plants grown under conditions of copper excess

Sánchez-Pardo

Fernández‐Pascual

Zornoza

2012

Environmental and Experimental Botany

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“…Many physiological disorders in plants are promoted by Cd-stress (Sanità di Toppi and Gabbrielli, 1999). In Cd-treated leguminous plants, structural alterations in leaves and roots have been reported (Vázquez et al, 1992;Vázquez et al, 2007), as well as in the root nodules of pea (Ausili et al, 2002), soybean (Chen et al, 2003) and white lupin (Carpena et al, 2003). Although the levels of Cd in agricultural soils are usually low, the metal has adverse effects on soil biological activity, reducing nodule formation and impairing nodule functioning in leguminous crops such as alfalfa (Porter and Sheridan, 1981), pea (Hernández et al, 1995), soybean (Balestrasse et al, 2001;Chen et al, 2003), white clover (Obbard and Jones, 1993) and white lupin (Zornoza et al, 2002).…”

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confidence: 99%

Cadmium in white lupin nodules: Impact on nitrogen and carbon metabolism

Sánchez-Pardo

Carpena

Zornoza

2013

Journal of Plant Physiology

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Esta es la versión de autor del artículo publicado en: This is an author produced version of a paper published in: The aims of this work were to investigate the microlocalisation of cadmium (Cd) in Lupinus albus L. cv. Multolupa nodules, and to determine its effects on carbon and nitrogen metabolism. Nodulated white lupin plants were grown in a growth chamber with or without Cd (150 µM). Energy-dispersive X-ray microanalysis showed the walls of the outer nodule cortex cells to be the main area of Cd retention, helping to reduce the harmful effect Cd might have on the amount of N 2 fixed by the bacteroids. Sucrose synthase activity declined by 33% in the nodules of the Cd-treated plants, and smaller reductions were recorded in glutamine synthetase, aspartate aminotransferase, alkaline invertase and NADP-dependent isocitrate dehydrogenase activities. The Cd treatment also sharply reduced nodule concentrations of malate, succinate and citrate, while that of starch doubled, but that of sucrose experienced no significant change. In summary, the present results show that white lupins accumulate significant amounts of Cd in their root nodules. However, the activity of some enzymes involved in ammonium assimilation did decline, promoting a reduction in the plant N content. The downregulation of sucrose synthase limits the availability of carbon to the bacteroids, which might interfere with their respiration. Carbon metabolism therefore plays a primary role in the impaired function of the white lupin root nodule caused by Cd, while N metabolism appears to have a more secondary involvement. Other plants, most notably legumes, can also obtain N from atmospheric N 2 via the entry of the roots into an endosymbiotic association with nitrogen-fixing bacteria (Stougaard, 2000). This interaction gives rise to a new plant organ: the root nodule.These nodules provide optimal living conditions for nitrogen-fixing bacteria, which reduce N 2 to NH 4 + via the action of nitrogenase (Nase) (Temple et al., 1998). Once NH 4 + is formed it binds to glutamic acid (in the presence of ATP and via the action of glutamine synthetase [GS]), to form glutamine. Glutamine can then form two glutamic acid molecules in the presence of oxoglutarate and glutamate synthase (glutamine-2-oxoglutarate-amino-transferase; GOGAT). The glutamine synthetase/glutamate synthase (GS/GOGAT) system is of vital importance since the glutamine and glutamate produced act as N donors for the biosynthesis of major N-containing compounds such as amino acids, nucleotides and chlorophylls (Lea and Ireland, 1999). The symbiotic fixation of N 2 is dependent on the supply of carbohydrates by the host plant to the bacteroids in the root nodules. Photosynthates, predominantly sucrose, are translocated from the shoots to the root nodules, providing the bacteroids the energy and reductants required by Nase, and the carbon skeletons needed for the assimilation of NH 4 + . N 2 fixing bacteria-legume symbiosis is therefore based on the exchange of carbon and nitrogen between the ...

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Synergistic effects of biochar and biostimulants on nutrient and toxic element uptake by pepper in contaminated soils

et al. 2021

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BACKGROUND Nowadays a significant amount of land contaminated with toxic elements is being used for agriculture, posing a serious risk of crop contamination and toxicity. Several methodologies are being used to remediate soil contamination, including the use of amendments such as biochar. This work evaluated the effects of biochar combined with different fertirrigations (water, a conventional fertilizer solution, or a fertilizer solution with a commercial biostimulant derived from leonardite) on the availability of toxic elements and nutrients for pepper cultivated in a soil contaminated with As, Cd, Pb, and Zn. RESULTS Irrigation with fertilizer solutions improved plant growth regardless of the biochar amendment. Biochar decreased the bioavailability of Cu and Pb in soil and the Cu content in pepper leaves. Combined with fertilization, biochar also decreased plant As and Pb content. Biochar combined with biostimulant decreased the bioavailable content of Cd in soil and its uptake by pepper plants. CONCLUSION The use of biochar and biostimulant presented advantages for plant production in a non‐suitable scenario of nutrient scarcity and contamination. © 2021 The Authors. Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

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Cadmium-stress in white lupin: effects on nodule structure and functioning

Cited by 67 publications

References 36 publications

Copper microlocalisation, ultrastructural alterations and antioxidant responses in the nodules of white lupin and soybean plants grown under conditions of copper excess

Copper microlocalisation, ultrastructural alterations and antioxidant responses in the nodules of white lupin and soybean plants grown under conditions of copper excess

Cadmium in white lupin nodules: Impact on nitrogen and carbon metabolism

Synergistic effects of biochar and biostimulants on nutrient and toxic element uptake by pepper in contaminated soils

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