Cadmium-stress in nodulated white lupin: strategies to avoid toxicity

Zornoza, Pilar; Vázquez, Saúl; Esteban, Elvira; Fernández‐Pascual, Mercedes; Carpena, Ramón O.

doi:10.1016/s0981-9428(02)01464-x

Cited by 182 publications

(126 citation statements)

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“…In contrast, Cd had no effect on root development when an adequate Mn supply was available, although slight symptoms of Cd toxicity were visible in the shoots of these plants (Tables 2 and 4). Previous investigations into Cd stress in white lupins grown with Cd doses of ≤45 M found virtually no growth problems (Zornoza et al, 2002).…”

Section: Discussionmentioning

confidence: 92%

“…The formation of proteoid roots appears to be mainly induced by a shortage of P and, at least in some plant species, by Fe deficiency (Dinkelaker et al, 1995;Hagström et al, 2001). Earlier studies into Cd stress in white lupins grown with Cd doses of ≤45 M found strong reductions of P and Fe plant accumulation, but proteoid roots did not appear in Cd stressed plants (Zornoza et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

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Interaction and accumulation of manganese and cadmium in the manganese accumulator Lupinus albus

Zornoza

Sánchez-Pardo

Carpena

2010

Journal of Plant Physiology

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Section: Discussionmentioning

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Interaction and accumulation of manganese and cadmium in the manganese accumulator Lupinus albus

Zornoza

Sánchez-Pardo

Carpena

2010

Journal of Plant Physiology

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“…Many physiological disorders in plants are promoted by Cd-stress (Sanità di Toppi and Gabbrielli, 1999). In Cd-treated leguminous plants, structural alterations in leaves and roots have been reported (Vázquez et al, 1992;Vázquez et al, 2007), as well as in the root nodules of pea (Ausili et al, 2002), soybean (Chen et al, 2003) and white lupin (Carpena et al, and As (Zornoza et al, 2002;Esteban et al, 2008;Vazquez et al, 2009), suggest it to be a suitable organism for use in the remediation of contaminated soils. While Cd excess is known to reduce the formation of root nodules in legumes, little is known about how the symbiotic union between legumes and N 2 -fixing bacteria is affected by high Cd concentrations.…”

Section: Introductionmentioning

confidence: 99%

“…In Cd-treated leguminous plants, structural alterations in leaves and roots have been reported (Vázquez et al, 1992;Vázquez et al, 2007), as well as in the root nodules of pea (Ausili et al, 2002), soybean (Chen et al, 2003) and white lupin (Carpena et al, 2003). Although the levels of Cd in agricultural soils are usually low, the metal has adverse effects on soil biological activity, reducing nodule formation and impairing nodule functioning in leguminous crops such as alfalfa (Porter and Sheridan, 1981), pea (Hernández et al, 1995), soybean (Balestrasse et al, 2001;Chen et al, 2003), white clover (Obbard and Jones, 1993) and white lupin (Zornoza et al, 2002). (Lupinus).…”

mentioning

confidence: 99%

“…Lupin nodules are recognised as a special subclass of indeterminate nodules, known as lupinoid nodules . The ability of white lupin to survive in soils of low pH and low nutrient availability, and the species' intrinsic biomass production and relative tolerance to trace elements such as Cd, Hg 5 and As (Zornoza et al, 2002; Esteban et al, 2008; Vazquez et al, 2009), suggest it to be a suitable organism for use in the remediation of contaminated soils. While Cd excess is known to reduce the formation of root nodules in legumes, little is known about how the symbiotic union between legumes and N 2 -fixing bacteria is affected by high Cd concentrations.…”

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confidence: 99%

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Cadmium in white lupin nodules: Impact on nitrogen and carbon metabolism

Sánchez-Pardo

Carpena

Zornoza

2013

Journal of Plant Physiology

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Esta es la versión de autor del artículo publicado en: This is an author produced version of a paper published in: The aims of this work were to investigate the microlocalisation of cadmium (Cd) in Lupinus albus L. cv. Multolupa nodules, and to determine its effects on carbon and nitrogen metabolism. Nodulated white lupin plants were grown in a growth chamber with or without Cd (150 µM). Energy-dispersive X-ray microanalysis showed the walls of the outer nodule cortex cells to be the main area of Cd retention, helping to reduce the harmful effect Cd might have on the amount of N 2 fixed by the bacteroids. Sucrose synthase activity declined by 33% in the nodules of the Cd-treated plants, and smaller reductions were recorded in glutamine synthetase, aspartate aminotransferase, alkaline invertase and NADP-dependent isocitrate dehydrogenase activities. The Cd treatment also sharply reduced nodule concentrations of malate, succinate and citrate, while that of starch doubled, but that of sucrose experienced no significant change. In summary, the present results show that white lupins accumulate significant amounts of Cd in their root nodules. However, the activity of some enzymes involved in ammonium assimilation did decline, promoting a reduction in the plant N content. The downregulation of sucrose synthase limits the availability of carbon to the bacteroids, which might interfere with their respiration. Carbon metabolism therefore plays a primary role in the impaired function of the white lupin root nodule caused by Cd, while N metabolism appears to have a more secondary involvement. Other plants, most notably legumes, can also obtain N from atmospheric N 2 via the entry of the roots into an endosymbiotic association with nitrogen-fixing bacteria (Stougaard, 2000). This interaction gives rise to a new plant organ: the root nodule.These nodules provide optimal living conditions for nitrogen-fixing bacteria, which reduce N 2 to NH 4 + via the action of nitrogenase (Nase) (Temple et al., 1998). Once NH 4 + is formed it binds to glutamic acid (in the presence of ATP and via the action of glutamine synthetase [GS]), to form glutamine. Glutamine can then form two glutamic acid molecules in the presence of oxoglutarate and glutamate synthase (glutamine-2-oxoglutarate-amino-transferase; GOGAT). The glutamine synthetase/glutamate synthase (GS/GOGAT) system is of vital importance since the glutamine and glutamate produced act as N donors for the biosynthesis of major N-containing compounds such as amino acids, nucleotides and chlorophylls (Lea and Ireland, 1999). The symbiotic fixation of N 2 is dependent on the supply of carbohydrates by the host plant to the bacteroids in the root nodules. Photosynthates, predominantly sucrose, are translocated from the shoots to the root nodules, providing the bacteroids the energy and reductants required by Nase, and the carbon skeletons needed for the assimilation of NH 4 + . N 2 fixing bacteria-legume symbiosis is therefore based on the exchange of carbon and nitrogen between the ...

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Genetic Engineering for Metal Tolerance and Accumulation

2022

Aquatic Environmental Bioengineering

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Cadmium-stress in nodulated white lupin: strategies to avoid toxicity

Cited by 182 publications

References 26 publications

Interaction and accumulation of manganese and cadmium in the manganese accumulator Lupinus albus

Interaction and accumulation of manganese and cadmium in the manganese accumulator Lupinus albus

Cadmium in white lupin nodules: Impact on nitrogen and carbon metabolism

Genetic Engineering for Metal Tolerance and Accumulation

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