Cadmium resistance and uptake by bacterium, Salmonella enterica 43C, isolated from industrial effluent

Abstract

Cadmium resistant bacterium, isolated from industrial wastewater, was characterized as Salmonella enterica 43C on the basis of biochemical and 16S rRNA ribotyping. It is first ever reported S. enterica 43C bared extreme resistance against heavy metal consortia in order of Pb2+>Cd2+>As3+>Zn2+>Cr6+>Cu2+>Hg2+. Cd2+ stress altered growth pattern of the bacterium in time dependent manner. It could remove nearly 57 % Cd2+ from the medium over a period of 8 days. Kinetic and thermodynamic studies based on various adsorption isotherm models (Langmuir and Freundlich) depicted the Cd2+ biosorption as spontaneous, feasible and endothermic in nature. Interestingly, the bacterium followed pseudo first order kinetics, making it a good biosorbent for heavy metal ions. The S. enterica 43C Cd2+ processivity was significantly influenced by temperature, pH, initial Cd2+ concentration, biomass dosage and co-metal ions. FTIR analysis of the bacterium revealed the active participation of amide and carbonyl moieties in Cd2+ adsorption confirmed by EDX analysis. Electron micrographs beckoned further surface adsorption and increased bacterial size due to intracellular Cd2+ accumulation. An overwhelming increase in glutathione and other non-protein thiols levels played a significant role in thriving oxidative stress generated by metal cations. Presence of metallothionein clearly depicted the role of such proteins in bacterial metal resistance mechanism. The present study results clearly declare S. enterica 43C a suitable candidate for green chemistry to bioremediate environmental Cd2+.Electronic supplementary materialThe online version of this article (doi:10.1186/s13568-016-0225-9) contains supplementary material, which is available to authorized users.

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