Cadmium ion-chlorophyll interaction – Examination of spectral properties and structure of the cadmium-chlorophyll complex and their relevance to photosynthesis inhibition

Grajek, Hanna; Rydzyński, Dariusz; Piotrowicz-Cieślak, Agnieszka I.; Herman, Aleksander; Maciejczyk, Maciej; Wieczorek, Z

doi:10.1016/j.chemosphere.2020.127434

Cited by 51 publications

(15 citation statements)

References 35 publications

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“…Over the past century, various human activities have resulted in environmental Cd pollution (Rahimzadeh et al, 2017 ; Genchi et al, 2020a , b ). Characterized by strong bioaccumulation, high bioavailability, and strong biotoxicity, Cd can cause the wilting of leaves, the browning of roots, and even the death of plant cells, leading to a decline in plant yield and quality (Sarangthem et al, 2011 ; Wang et al, 2019 ; Grajek et al, 2020 ). Cd accumulates in the human body through the food chain and causes irreversible damage (Valverde et al, 2001 ; Satarug et al, 2003 ; Filipič, 2012 ).…”

Section: Introductionmentioning

confidence: 99%

Untargeted LC–MS/MS-Based Metabolomic Profiling for the Edible and Medicinal Plant Salvia miltiorrhiza Under Different Levels of Cadmium Stress

et al. 2022

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Salvia miltiorrhiza, a medicinal and edible plant, has been extensively applied to treat cardiovascular diseases and chronic hepatitis. Cadmium (Cd) affects the quality of S. miltiorrhiza, posing serious threats to human health. To reveal the metabolic mechanisms of S. miltiorrhiza's resistance to Cd stress, metabolite changes in S. miltiorrhiza roots treated with 0 (CK), 25 (T1), 50 (T2) and 100 (T3) mg kg−1 Cd by liquid chromatography coupled to mass spectrometry (LC–MS/MS) were investigated. A total of 305 metabolites were identified, and most of them were amino acids, organic acids and fatty acids, which contributed to the discrimination of CK from the Cd-treated groups. Among them, S. miltiorrhiza mainly upregulated o-tyrosine, chorismate and eudesmic acid in resistance to 25 mg kg−1 Cd; DL-tryptophan, L-aspartic acid, L-proline and chorismite in resistance to 50 mg kg−1 Cd; and L-proline, L-serine, L-histidine, eudesmic acid, and rosmarinic acid in resistance to 100 mg kg−1 Cd. It mainly downregulated unsaturated fatty acids (e.g., oleic acid, linoleic acid) in resistance to 25, 50, and 100 mg kg−1 Cd and upregulated saturated fatty acids (especially stearic acid) in resistance to 100 mg kg−1 Cd. Biosynthesis of unsaturated fatty acids, isoquinoline alkaloid, betalain, aminoacyl-tRNA, and tyrosine metabolism were the significantly enriched metabolic pathways and the most important pathways involved in the Cd resistance of S. miltiorrhiza. These data elucidated the crucial metabolic mechanisms involved in S. miltiorrhiza Cd resistance and the crucial metabolites that could be used to improve resistance to Cd stress in medicinal plant breeding.

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Section: Introductionmentioning

confidence: 99%

Untargeted LC–MS/MS-Based Metabolomic Profiling for the Edible and Medicinal Plant Salvia miltiorrhiza Under Different Levels of Cadmium Stress

et al. 2022

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“…A statistically significant difference in chlorophyll fluorescence was observed between cc4425 and cc5013 (6,127 and 3,858 counts respectively) at 10 μM Cd (Figure 2). A possible mechanism behind the phenomenon reported in cc5013 is a decrease in chlorophyll intensity level because of Cd substituting magnesium in chlorophyll, leading to the decrease in chlorophyll intensity (Grajek et al, 2020;Rydzy nski et al, 2019;Kupper et al, 1996). The apparent absence of this phenomenon in cc4425 at the Cd concentrations used in this study might be, at least in part, contributed to by the biological activity of CIA7.…”

Section: Discussionmentioning

confidence: 60%

Comparison of cell size, chlorophyll fluorescence and cadmium (Cd2+) bioaccumulation between wild-type and mutant strains of Chlamydomonas reinhardtii upon exposure to Cd2

2021

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Background: Heavy metal contamination presents a constant threat to biological systems. Simultaneously, heavy metals have become one of the major contaminants in the aquatic ecosystems. In this regard, the investigation of heavy metal-tolerance genes in algae is relevant. Chlamydomonas reinhardtii is a unicellular green alga, and an excellent model organism used in heavy metal studies. In C. reinhardtii, a novel gene designated as Cia7, was hypothesized to play a role in heavy metal homeostasis due to CIA7’s conserved cysteine-residue motif. This study compared two strains of C. reinhardtii, cc4425, the wild-type with the functional CIA7 protein and cc5013, the mutant strain with the disrupted cia7- gene. The hypothesis was that the expression of Cia7 contributes to an increased cadmium (Cd)-tolerance in C. reinhardtii. The Cd-tolerance would be described by physiological markers of microalgae health, and by intracellular accumulation of the metal. Methods: The objectives of this study were (1) to compare chlorophyll fluorescence and cell size in cc4425 and cc5013 exposed to Cd2+, and (2) to compare Cd2+ bioaccumulation in cc4425 and cc5013 strains in different growth media. Flow cytometry, and inductively coupled plasma optical emission spectrometry (ICP-OES) analysis were performed. Results: There was no significant statistical difference in Cd2+ bioaccumulation between the two strains, cc4425 and cc5013, regardless of growth media. However, a statistically significant difference in Cd2+ bioaccumulation (p<0.0001) was determined between the media (with acetate and without acetate). The cia7- mutant, cc5013 was found to be more susceptible to a Cd2+-induced decrease in chlorophyll fluorescence and had a reduced cell size compared to cc4425, the wild-type strain. Conclusions: These observed differences between the strains suggest that CIA7’s biological activity could play a direct or indirect role in increasing Cd tolerance in C. reinhardtii.

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“…It was suggested that metal toxicity on photosynthesis is related to the binding ability of the photosynthetic apparatus to metals [ 67 ]. Therefore, the binding reaction between photosystems and heavy metal ions may explain their different toxicity.…”

Section: Discussionmentioning

confidence: 99%

Cadmium Caused Different Toxicity to Photosystem I and Photosystem II of Freshwater Unicellular Algae Chlorella pyrenoidosa (Chlorophyta)

Wang

Wufuer

Duo

et al. 2022

Toxics

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Heavy metals such as Cd pose environmental problems and threats to a variety of organisms. The effects of cadmium (Cd) on the growth and activities of photosystem I (PSI) and photosystem II (PSII) of Chlorella pyrenoidosa were studied. The growth rate of cells treated with 25 and 100 µM of Cd for longer than 48 h were significantly lower than the control, accompanying with the inhibition of photosynthesis. The result of quantum yields and electron transport rates (ETRs) in PSI and PSII showed that Cd had a more serious inhibition on PSII than on PSI. Cd decreased the efficiency of PSII to use the energy under high light with increasing Cd concentration. In contrast, the quantum yield of PSI did not show a significant difference among different Cd treatments. The activation of cyclic electron flow (CEF) and the inhibition of linear electron flow (LEF) due to Cd treatment were observed. The photochemical quantum yield of PSI and the tolerance of ETR of PSI to Cd treatments were due to the activation of CEF around PSI. The activation of CEF also played an important role in induction of non-photochemical quenching (NPQ). The binding features of Cd ions and photosystem particles showed that Cd was easier to combine with PSII than PSI, which may explain the different toxicity of Cd on PSII and PSI.

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Cadmium ion-chlorophyll interaction – Examination of spectral properties and structure of the cadmium-chlorophyll complex and their relevance to photosynthesis inhibition

Cited by 51 publications

References 35 publications

Untargeted LC–MS/MS-Based Metabolomic Profiling for the Edible and Medicinal Plant Salvia miltiorrhiza Under Different Levels of Cadmium Stress

Untargeted LC–MS/MS-Based Metabolomic Profiling for the Edible and Medicinal Plant Salvia miltiorrhiza Under Different Levels of Cadmium Stress

Comparison of cell size, chlorophyll fluorescence and cadmium (Cd2+) bioaccumulation between wild-type and mutant strains of Chlamydomonas reinhardtii upon exposure to Cd2

Cadmium Caused Different Toxicity to Photosystem I and Photosystem II of Freshwater Unicellular Algae Chlorella pyrenoidosa (Chlorophyta)

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