Cadmium induces impaired glucose tolerance in rat by down-regulating GLUT4 expression in adipocytes

Han, Jin Chul; Park, Seung Yeon; Hah, Byoung G; Choi, Geum Hee; Kim, Yung K.; Kwon, Tae Hwan; Kim, Eui K; Lachaal, Mohsen; Jung, Chan Y.; Lee, Wan

doi:10.1016/s0003-9861(03)00120-6

Cited by 96 publications

(70 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Cd-induced cellular toxicity has been described in various targets including metalloenzyme interferences, thiol protein alterations, inhibition of energy metabolism, DNA and membrane structure/function alterations, and excessive oxidative damage [52]. Several studies have shown that Cd-induced hyperglycemia was associated with increased lipid peroxidation, decreased insulin release, increased activation of gluconeogenic enzymes and impaired insulin receptor [53,54]. The high Pb burden is a predictor of prospective increases in complexity of diabetes.…”

Section: Discussionmentioning

confidence: 99%

Comparative metal distribution in scalp hair of Pakistani and Irish referents and diabetes mellitus patients

Afridi

Kazi

Brabazon

et al. 2013

Clinica Chimica Acta

View full text Add to dashboard Cite

Background: The essential metals, chromium (Cr), magnesium (Mg), manganese (Mn) and zinc (Zn), are necessary for many metabolic processes and their homeostasis is crucial for life. The toxic metals, cadmium (Cd) and lead (Pb), have no beneficial role in human metabolism. The aim of this study was to investigate the levels of Cd, Cr, Mg, Mn, Pb, and Zn in scalp hair samples of type 2 diabetes mellitus patients of both genders, ages ranging from 30 to 50 y, and belong to urban areas of Ireland and Pakistan. For comparison purposes, age matched non-diabetic subjects of both countries were selected as referents. Methods: The concentrations of metals in scalp hair samples were measured by inductively coupled plasma atomic emission spectrophotometer and atomic absorption spectrophotometer after microwave-assisted acid digestion. The validity and accuracy of the methodology were checked by conventional wet-acid-digestion method and using certified reference materials. Results: The mean values of Cd and Pb were significantly higher in scalp hair samples of both Pakistani and Irish diabetic patients as compared to referents of both countries (P<0.001). In contrast, lower Cr, Mg, Mn, and Zn (P<0.01) concentrations were detected in scalp hair derived from patients with type 2 diabetes versus healthy subjects of both countries. Conclusion: This study showed that, increased toxic elements and decreased essential elements are associated with diabetes mellitus. Therefore, these elements may play a role in the development and pathogenesis of diabetes mellitus.

show abstract

Section: Discussionmentioning

confidence: 99%

Comparative metal distribution in scalp hair of Pakistani and Irish referents and diabetes mellitus patients

Afridi

Kazi

Brabazon

et al. 2013

Clinica Chimica Acta

View full text Add to dashboard Cite

show abstract

“…Also, cadmium has been shown to impair glucose tolerance in rats. 78 Other studies have indicated that cadmium exposure caused a metal accumulation, and induced degeneration, necrosis, and weak degranulation in the pancreatic β-cells. 81,82 Thus, cadmium exposure might cause diabetic symptoms through the induction of oxidative stress and disruption of islet β-cell function.…”

Section: Cadmiummentioning

confidence: 98%

“…Cadmium-induced cellular toxicity has been described in various targets including metalloenzymes interference, thiol protein alterations, energy metabolism inhibition, DNA and membrane structure/function alterations, and excessive oxidative damage. 68,69,[76][77][78] Moreover, several studies have shown that cadmium-induced hyperglycemia was associated with increased lipid peroxidation, decreased insulin release, increased activation of gluconeogenic enzymes and impaired insulin receptor. 74,[78][79][80] Cadmium has also been demonstrated to induce a dose-dependent reduction in GLUT4 protein and mRNA expressions in rat adipocytes.…”

Section: Cadmiummentioning

confidence: 99%

Heavy metals, islet function and diabetes development

Lin¹,

Yang²,

Huang³

et al. 2009

Islets

204

131

View full text Add to dashboard Cite

“…The most important rout to reduce blood glucose concentrations are glucose exposal as a resource of cell energy and glycogen synthesis following its uptake into the cell via glucose transporters or GLUTs which are trans-membrane glycoproteins; this process essentially is mediated by insulin [5][6][7]. Adipocytes and myocytes are two major glucose disposing tissues [7,8] that their most important and abundant glucose transporter is GLUT4 which as same as other glucose transporters facilitates glucose transport from extracellular environment into the cytosol [9]; Transcription and intracellular movement of GLUT4 gene product into the cytoplasmic membrane mediated by insulin signal transduction pathway; So GLUT4 dependent glucose transport is considered insulin dependent [7,8].…”

Section: Introductionmentioning

confidence: 99%

Hydro-Alcoholic Cinnamon Extract, Enhances Glucose Transporter Isotype-4 Translocation from Intracellular Compartments into the Cytoplasmic Membrane of C2C12 Myotubes

et al. 2012

View full text Add to dashboard Cite

Cinnamon has been used as an anti-diabetic agent for centuries but only in recent few years its mechanism of action has been under investigation. Previous studies showed that cinnamon might exert its anti-diabetic effect via increasing glucose transporter isotype-4 (GLUT4) gene and glycoprotein contents in fat cells. To study if hydro-alcoholic cinnamon extract (HACE) enhances GLUT4 translocation from intracellular compartments of nuclear or endoplasmic reticulum membranes (N/ER) into the cytoplasmic membrane (CM). C2C12 myoblastic cell line were seeded in DMEM plus 20 % FBS and differentiated to myotubes using 2 % horse serum. After myotubes formation, 100 or 1,000 lg/ml HACE, as intervention, and as control 1 % DMSO were added for 3 h. Cells were washed and homogenized followed by ultracentrifuge fractionation, protein separation by SDS-PAGE and GLUT4 detection using semi-quantitative Western blotting. Data analysis was done by two-independent samples t test for comparison of mean ± SD of GLUT4 percent in categories. GLUT4 contents were higher in CM of groups 100 and 1,000 lg/ml HACE and lower in 1 % DMSO treated myotubes (CI = 0.95, P \ 0.05). For N/ER reverse results were obtained (CI = 0.95, P \ 0.05). As our results have shown HACE induces GLUT4 translocation from intra-cell into cell surface. We conclude that cinnamon maybe a choice of type-2 diabetes mellitus treatment because its extract enhances GLUT4 contents in CM where it facilitates glucose entrance into the cell. However it is necessary to trace the signaling pathways which are activated by HACE in muscular tissue.

show abstract

Cadmium induces impaired glucose tolerance in rat by down-regulating GLUT4 expression in adipocytes

Cited by 96 publications

References 44 publications

Comparative metal distribution in scalp hair of Pakistani and Irish referents and diabetes mellitus patients

Comparative metal distribution in scalp hair of Pakistani and Irish referents and diabetes mellitus patients

Heavy metals, islet function and diabetes development

Hydro-Alcoholic Cinnamon Extract, Enhances Glucose Transporter Isotype-4 Translocation from Intracellular Compartments into the Cytoplasmic Membrane of C2C12 Myotubes

Contact Info

Product

Resources

About