Cadmium-induced ovarian pathophysiology is mediated by change in gene expression pattern of zinc transporters in zebrafish (Danio rerio)

Chouchene, Lina; Bannı, Mohamed; Kerkeni, Abdelhamid; Saïd, Khaled; Messaoudi, Imed

doi:10.1016/j.cbi.2011.06.010

Cited by 47 publications

(27 citation statements)

References 49 publications

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“…b p<0.01, statistical significance between Cd and Cd+Zn treatment expressions in the two transport pathway but decreased the expression of ZnT2 and ZnT4 at PND 14 and PND 21 only in the mammary tissue. This is in accordance with previous data of our group [28] which indicated that Cd exposure caused upregulation of ZIP10 gene expression and decreased the expression of ZnT1 in zebrafish ovaries. The effect of Cd on Zn transporter gene expression on the mammary gland may lead to less transfer of the essential element from maternal circulation to the milk and could partly explain the observed decrease of Zn levels in the stomach content of pups at PND 14 found in the present study.…”

Section: Discussionsupporting

confidence: 95%

“…Several studies have suggested that the toxic effects of Cd may be mediated by altered metabolism of essential elements including Zn [18,27,28]. The present study shows that Cd exposure during lactation decrease significantly the neonatal Zn absorption and leads to Zn redistribution in suckling rat organism, with increase in its total content in the liver and kidneys and its depletion in the gastrointestinal tract (stomach content and intestine) and plasma.…”

Section: Discussionmentioning

confidence: 59%

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Changes of the mRNA Expression Pattern of Zn Transporters: a Probable Mechanism for Cadmium Retention and Zinc Redistribution in the Suckling Rat Tissues

Chemek

Boughammoura

Mimouna

et al. 2015

Biol Trace Elem Res

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The present study was conducted to provide potential mechanism that may be responsible for Cd retention and Cd-induced Zn redistribution in tissues of suckling rat. For this purpose, suckling rats from mother receiving either tap water, Cd, or Cd + Zn during lactation period were sacrificed on postnatal day (PND) 14 and PND 21 for performing chemical and molecular analysis. Our results show that Cd exposure, although it does not affect the milk consumption, it clearly alters the lactational transfer, absorption, and distribution of Zn in the suckling rat organism. At the molecular level, Cd caused upregulation of ZIP 3, ZIP 4, and ZIP 8 gene expressions in the mammary gland of mothers rats and in the intestine of their pups but decreased the expression of ZnT 2 and ZnT 4 only in the mammary tissue at PND 14 and PND 21. Zn supply reversed the Cd-induced decrease in the neonatal Zn apparent absorption and restores the gastrointestinal, brain, and plasma levels of this essential element in the suckling rat organism at PND 14 and PND 21. Also, with this treatment, the gene expressions of ZnT 1 in the mammary gland and ZnT 4 in the neonatal intestine were found to be upregulated at PND 21. Furthermore, our results show that Cd or Cd + Zn treatment increase the neonatal hepatic MTs accumulation at PND 14 only. These results imply that the downregulation of ZnT as well as the overexpression of ZIP transporters, in responses to the Zn depletion induced by Cd in the tissues of lactating rat and their offspring, play a major role in Cd accumulation and Zn redistribution in tissues of suckling rat.

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Section: Discussionsupporting

confidence: 95%

Section: Discussionmentioning

confidence: 59%

Changes of the mRNA Expression Pattern of Zn Transporters: a Probable Mechanism for Cadmium Retention and Zinc Redistribution in the Suckling Rat Tissues

Chemek

Boughammoura

Mimouna

et al. 2015

Biol Trace Elem Res

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“…Gonadal damage and interference with gametogenesis were also observed after Cd exposure. In testis, Cd can cause hemorrhagic necrosis (Parizek & Zahor, ; Thompson & Bannigan, ) and reduced sperm count (Amara et al , ), whereas in ovaries, it increases the proportion of atretic follicles, reduces the number of vitellogenic oocytes and decreases yolk formation (Chouchene et al , ). Several other studies have also associated Cd with a wide range of reproductive disorders, including growth retardation, sterility and embryotoxicity (Fahmy & Aly, ).…”

Section: Introductionmentioning

confidence: 99%

“…Indeed, numerous studies have shown that Zn strongly reversed Cd‐induced toxicity in various organs and tissues such as liver (Banni et al , ), kidney (El Heni et al , ), blood (Jemai et al , ) and testis (Messaoudi et al , ). Using adult female zebrafish, we have recently reported similar results in the ovarian tissue (Chouchene et al , ). Zn treatment during Cd exposure reversed Cd‐induced Zn depletion, as well as Cd and metallothionein accumulation, thus reducing ovarian histopathological damages (Chouchene et al , ).…”

Section: Introductionmentioning

confidence: 99%

Inhibitory effect of cadmium on estrogen signaling in zebrafish brain and protection by zinc

Chouchene¹,

Pellegrini

Guéguen

et al. 2016

J of Applied Toxicology

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The present study was conducted to assess the effects of Cd exposure on estrogen signaling in the zebrafish brain, as well as the potential protective role of Zn against Cd-induced toxicity. For this purpose, the effects on transcriptional activation of the estrogen receptors (ERs), aromatase B (Aro-B) protein expression and molecular expression of related genes were examined in vivo using wild-type and transgenic zebrafish embryos. For in vitro studies, an ER-negative glial cell line (U251MG) transfected with different zebrafish ER subtypes (ERα, ERβ1 and ERβ2) was also used. Embryos were exposed either to estradiol (E2 ), Cd, E2 +Cd or E2 +Cd+Zn for 72 h and cells were exposed to the same treatments for 30 h. Our results show that E2 treatment promoted the transcriptional activation of ERs and increased Aro-B expression, at both the protein and mRNA levels. Although exposure to Cd, does not affect the studied parameters when administered alone, it significantly abolished the E2 -stimulated transcriptional response of the reporter gene for the three ER subtypes in U251-MG cells, and clearly inhibited the E2 induction of Aro-B in radial glial cells of zebrafish embryos. These inhibitory effects were accompanied by a significant downregulation of the expression of esr1, esr2a, esr2b and cyp19a1b genes compared to the E2 -treated group used as a positive control. Zn administration during simultaneous exposure to E2 and Cd strongly stimulated zebrafish ERs transactivation and increased Aro-B protein expression, whereas mRNA levels of the three ERs as well as the cyp19a1b remained unchanged in comparison with Cd-treated embryos. In conclusion, our results clearly demonstrate that Cd acts as a potent anti-estrogen in vivo and in vitro, and that Cd-induced E2 antagonism can be reversed, at the protein level, by Zn supplement. Copyright © 2016 John Wiley & Sons, Ltd.

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“…Finally, the gene slc39a6 codes for a zinc influx ion transmembrane transporter. Many studies have already shown that Cd exposure disrupts zinc homeostasis by increasing the transcription level of zinc transporters and zinc accumulation in the cell (Noel et al 2006;Chouchene et al 2011;Tang et al 2013).…”

Section: Fin Transcriptomic Response To Experimental CD and Pcb Exposmentioning

confidence: 98%

Detecting the exposure to Cd and PCBs by means of a non-invasive transcriptomic approach in laboratory and wild contaminated European eels (Anguilla anguilla)

Baillon

Pierron

Oses

et al. 2015

Environ Sci Pollut Res

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Detecting and separating specific effects of contaminants in a multi-stress field context remain a major challenge in ecotoxicology. In this context, the aim of this study was to assess the usefulness of a non-invasive transcriptomic method, by means of a complementary DNA (cDNA) microarray comprising 1000 candidate genes, on caudal fin clips. Fin gene transcription patterns of European eels (Anguilla anguilla) exposed in the laboratory to cadmium (Cd) or a polychloro-biphenyl (PCBs) mixture but also of wild eels from three sampling sites with differing contamination levels were compared to test whether fin clips may be used to detect and discriminate the exposure to these contaminants. Also, transcriptomic profiles from the liver and caudal fin of eels experimentally exposed to Cd were compared to assess the detection sensitivity of the fin transcriptomic response. A similar number of genes were differentially transcribed in the fin and liver in response to Cd exposure, highlighting the detection sensitivity of fin clips. Moreover, distinct fin transcription profiles were observed in response to Cd or PCB exposure. Finally, the transcription profiles of eels from the most contaminated site clustered with those from laboratory-exposed fish. This study thus highlights the applicability and usefulness of performing gene transcription assays on non-invasive tissue sampling in order to detect the in situ exposure to Cd and PCBs in fish.

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Cadmium-induced ovarian pathophysiology is mediated by change in gene expression pattern of zinc transporters in zebrafish (Danio rerio)

Cited by 47 publications

References 49 publications

Changes of the mRNA Expression Pattern of Zn Transporters: a Probable Mechanism for Cadmium Retention and Zinc Redistribution in the Suckling Rat Tissues

Changes of the mRNA Expression Pattern of Zn Transporters: a Probable Mechanism for Cadmium Retention and Zinc Redistribution in the Suckling Rat Tissues

Inhibitory effect of cadmium on estrogen signaling in zebrafish brain and protection by zinc

Detecting the exposure to Cd and PCBs by means of a non-invasive transcriptomic approach in laboratory and wild contaminated European eels (Anguilla anguilla)

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