Cadmium-induced genotoxicity in human osteoblast-like cells

Oliveira, Helena; Monteiro, Cristina; Pinho, Francisco; Pinho, Sónia; Oliveira, José Miguel P. Ferreira de; Santos, Conceição

doi:10.1016/j.mrgentox.2014.10.002

Cited by 36 publications

(15 citation statements)

References 66 publications

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“…As comet assay was significant by using metal concentrations lower than those inducing cytotoxicity, the value was determined by MTT assay at 2h. Then IC50 was found equal to The genotoxic impacts of cadmium and nickel confirmed previously published data [22,23], while the absence of genotoxicity of aluminum was also demonstrated [24]. However, the strength of the toxic action that occurred is different.…”

Section: Comet Assaysupporting

confidence: 81%

Direct DNA interaction and genotoxic impact of three metals: Cadmium, nickel and aluminum

Belliardo

Giorgio

Chaspoul

et al. 2018

The Journal of Chemical Thermodynamics

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HAL is a multidisciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L'archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d'enseignement et de recherche français ou étrangers, des laboratoires publics ou privés.

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Section: Comet Assaysupporting

confidence: 81%

Direct DNA interaction and genotoxic impact of three metals: Cadmium, nickel and aluminum

Belliardo

Giorgio

Chaspoul

et al. 2018

The Journal of Chemical Thermodynamics

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“…Cell cycle analysis was performed according to Oliveira et al [44], with minor modifications. Cells were seeded in 24-well plates, and after 24 h for adhesion were exposed for additional 24 h to two concentrations of NPs and metals, and also to binary mixtures of NPs and metals (same concentrations as before).…”

Section: Cell Cycle Analysismentioning

confidence: 99%

Unravelling the Potential Cytotoxic Effects of Metal Oxide Nanoparticles and Metal(Loid) Mixtures on A549 Human Cell Line

et al. 2020

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Humans are typically exposed to environmental contaminants’ mixtures that result in different toxicity than exposure to the individual counterparts. Yet, the toxicology of chemical mixtures has been overlooked. This work aims at assessing and comparing viability and cell cycle of A549 cells after exposure to single and binary mixtures of: titanium dioxide nanoparticles (TiO2NP) 0.75–75 mg/L; cerium oxide nanoparticles (CeO2NP) 0.75–10 μg/L; arsenic (As) 0.75–2.5 mg/L; and mercury (Hg) 5–100 mg/L. Viability was assessed through water-soluble tetrazolium (WST-1) and thiazolyl blue tetrazolium bromide (MTT) (24 h exposure) and clonogenic (seven-day exposure) assays. Cell cycle alterations were explored by flow cytometry. Viability was affected in a dose- and time-dependent manner. Prolonged exposure caused inhibition of cell proliferation even at low concentrations. Cell-cycle progression was affected by TiO2NP 75 mg/L, and As 0.75 and 2.5 μg/L, increasing the cell proportion at G0/G1 phase. Combined exposure of TiO2NP or CeO2NP mitigated As adverse effects, increasing the cell surviving factor, but cell cycle alterations were still observed. Only CeO2NP co-exposure reduced Hg toxicity, translated in a decrease of cells in Sub-G1. Toxicity was diminished for both NPs co-exposure compared to its toxicity alone, but a marked toxicity for the highest concentrations was observed for longer exposures. These findings prove that joint toxicity of contaminants must not be disregarded.

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“…Cell cycle analysis was performed according to the method described by Oliveira et al (2014) with slight modifications. Cells were plated in six-well plates at 2 × 10 5 cells/well and exposed to Cr(VI) as Four independent assays were performed, and for each sample, a minimum of 5000 events was recorded.…”

Section: Cell Cycle Analysismentioning

confidence: 99%

Cr(VI)‐induced genotoxicity and cell cycle arrest in human osteoblast cell line MG‐63

Monteiro

Santos

Bastos

et al. 2019

J of Applied Toxicology

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Occupational environments are major exposure routes to Cr(VI). However, Cr(VI) may also establish in bone tissues by ingestion or through Cr containing orthopaedic prostheses that, due to wear and corrosion, may release metal particles and ions potentially affecting bone tissue. The aim of this work was to evaluate the effects of clinically relevant concentrations of Cr(VI) in human osteoblasts, by integrating genotoxic effects, evaluated by the comet assay and cytokinesis‐blocked micronucleus assay (scoring the presence of micronucleus, nucleoplasmic bridges and nuclear division index), with the effects on cell cycle and cell viability. Human osteoblasts MG‐63 were in vitro exposed to Cr(VI) at concentrations ranging from 0.1 to 5 μm, for 24 and 48 hours. Results pointed out to a decrease of cell viability for both time exposures in a time‐ and dose‐dependent manner, which was related to cell cycle arrest and DNA damage. Chromosome abnormalities were also observed. Hence, these data suggest that cells arrested in the cell division with DNA damage may have followed cell death pathways, while some surviving ones still revealed DNA damage at chromosome level indicating abnormal cell division progression. In conclusion, Cr(VI) induced cytotoxic and genotoxic effects in human bone cells at concentrations that could be found in patients with metal‐on‐metal prostheses. In addition, the early onset of genotoxic damage induced by Cr(VI) at low concentrations after 24 hours of cell exposure alert to the relevance of periodic monitoring of patients for genotoxicity diagnosis after implantation of prostheses before clinical symptoms appear.

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Cadmium-induced genotoxicity in human osteoblast-like cells

Cited by 36 publications

References 66 publications

Direct DNA interaction and genotoxic impact of three metals: Cadmium, nickel and aluminum

Direct DNA interaction and genotoxic impact of three metals: Cadmium, nickel and aluminum

Unravelling the Potential Cytotoxic Effects of Metal Oxide Nanoparticles and Metal(Loid) Mixtures on A549 Human Cell Line

Cr(VI)‐induced genotoxicity and cell cycle arrest in human osteoblast cell line MG‐63

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