Cadmium-induced ER stress and inflammation are mediated through C/EBP–DDIT3 signaling in human bronchial epithelial cells

Kim, Jee Young; Song, Haengseok; Heo, Hye‐Ryeon; Kim, Jung Woon; Kim, Hye Ryun; Hong, Yoonki; Yang, Se‐Ran; Han, Seon-Sook; Lee, Seung-Joon; Kim, Woo Jin; Hong, Seok‐Ho

doi:10.1038/emm.2017.125

Cited by 36 publications

(23 citation statements)

References 39 publications

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“…Some studies attributed the increase in oxidative stress due to the production of free radicals as well as the induction of inflammation as a possible mechanism of cadmium in causing cellular damage as well as induction of cancer (Liu et al, 2011;Kim et al, 2017). Given the pathological effects of cadmium on renal cancer induction (kun Song et al, 2015) as well as the pivotal role of KLLN and PKHD1 genes in the incidence of kidney disease, in the present study, for the first time, the effect of crocin on the expression of PKHD1 and KLLN genes in renal tissue of male rats were tested for cadmium.…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies have identified the kidney as the most common organ involved with this element (Sá et al, 2016). Effect on message transmission (Thévenod., 2009), peroxidation of unsaturated fatty acids, inhibition of DNA repair system, inhibition of DNA methylation (Hartwig & Schwerdtle, 2002), depletion of antioxidants, dysfunction of E-cadherin protein, interaction with different ions, oxidative stress induced by free radicals overproduction (ROS) (Liu et al, 2011), inflammation and subsequent DNA oxidation, decreased levels of antioxidants, apoptosis disorder and increased activity of some proteases are potential mechanisms of toxic effects of cadmium on tissue damage (Kim et al, 2017;Mahdavi et al, 2011). Therefore, cadmium has been proposed as a candidate for alteration in the expression of effective genes resulting from kidney cancer (Chen et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

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Effect of crocin on PKHD1 and KLLN genes expression in kidney tissue of female rats treated with cadmium

Mohamdyari

Keshtmand

Moghadam

2020

IJMSS

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According to data from studies, antioxidant herbal compounds are, likely to have a useful role in reducing the harmful effects of environmental pollutants and toxic chemicals that most people are exposed to. Cadmium is one of the toxic elements that accumulate in many organs, especially in kidneys. The aim of this study was to investigate the effect of crocin on the expression of PKHD1 and KLLN genes in cadmium-treated rats. In this experimental study, 40 adults male Wistar rats (200-250 g) were randomly divided into the following groups: control group received normal saline, cadmium group (15mg/kg), crocin group (20mg/kg) and cadmium group daily fed with crocin at a dose of 20 mg/kg. After eight weeks of treatment, rats were dissected, and kidney tissues were removed for evaluation of PKHD1 and KLLN gene expression by real time method. The data were analyzed using oneway ANOVA and significant difference between groups was P<0.05. Our results showed an increase in PKHD1 gene expression and a decrease in KLLN gene expression in kidney tissue in the cadmium group compared to the control group (P <0.001). Also, a significant decrease in PKHD1 gene expression (P <0.001) and an increase in KLLN gene expression P <0.05) were observed in the tissues of all cadmium-treated rats compared to cadmium. Crocin consumption can have a protective effect against the impaired expression of PKHD1 and KLLN cadmium-induced apoptotic pathway.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effect of crocin on PKHD1 and KLLN genes expression in kidney tissue of female rats treated with cadmium

Mohamdyari

Keshtmand

Moghadam

2020

IJMSS

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“…Cadmium chloride, 0.1 M ER stress, unfolded protein response [27][28][29][30][31] Magnesium chloride, 0.1 M (as a divalent metal salt)…”

Section: Drosophila Strains and Treatmentsmentioning

confidence: 99%

Domesticated gag Gene of Drosophila LTR Retrotransposons Is Involved in Response to Oxidative Stress

Makhnovskii

Balakireva

Нефедова

et al. 2020

Genes

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Drosophila melanogaster is one of the most extensively used genetic model organisms for studying LTR retrotransposons that are represented by various groups in its genome. However, the phenomenon of molecular domestication of LTR retrotransposons has been insufficiently studied in Drosophila, as well as in other invertebrates. The present work is devoted to studying the role of the domesticated gag gene, Gagr, in the Drosophila genome. The Gagr gene has been shown to be involved in the response to stress caused by exposure to ammonium persulfate, but not in the stress response to oligomycin A, zeomycin, and cadmium chloride. Ammonium persulfate tissue specifically activates the expression of Gagr in the tissues of the carcass, but not in the gut. We found that the Gagr gene promoter contains one binding motif for the transcription factor kayak, a component of the JNK signaling pathway, and two binding motifs for the transcription factor Stat92E, a component of the Jak-STAT signaling pathway. Remarkably, Gagr orthologs contain the second binding motif for Stat92E only in D. melanogaster, D. simulans and D. sechellia, whereas in D. yakuba and D. erecta, Gagr orthologs contain a single motif, and there are no binding sites for Stat92E in the promoters of Gagr orthologs in D. ananassae and in species outside the melanogaster group. The data obtained indicate the formation of the protective function of the Gagr gene during evolution.

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“…BEAS-2B and NHBE cells were seeded at a density of 2.0×10 5 cells per well in 6-well plates and incubated until they reach 70-80% confluency. Cells were then transfected with miR-181a-2-3p mimics and/or inhibitors, followed by treatment with or without Cd for 24 h (27,28).…”

Section: Treatmentmentioning

confidence: 99%

“…Cd, a major component of cigarette smoke and particulate matter, induces inflammatory responses and endoplasmic reticulum stress in human bronchial epithelial cells via the C/EBP-DDIT3 signaling pathway (28). Thus, we investigated whether expression of miRNAs is influenced by Cd treatment in BEAS-2B and NHBE cells.…”

Section: Mir-181a-2-3p Is Downregulated In Lung Tissues and Serum Of mentioning

confidence: 99%

Role of miRNA-181a-2-3p in cadmium-induced inflammatory responses of human bronchial epithelial cells

Kim¹,

Kim²,

Heo³

et al. 2019

J. Thorac. Dis

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Background: Inflammation is an important priming event in the pathogenesis of pulmonary diseases, including chronic obstructive pulmonary disease (COPD). Increasing evidence indicates that microRNAs (miRNAs) contribute to the pathogenesis of COPD by regulating inflammatory response. Therefore, it is necessary to investigate novel molecular targets in COPD without any validation in COPD samples in airway inflammation. The aim of our study is to reveal novel miRNAs that can influence molecular targets for COPD and to examine the underlying mechanism in airway inflammation. Methods: We identified the downregulation of miR-181a-2-3p in the serum of COPD patients and further investigated the role of miR-181a-2-3p in cadmium (Cd)-induced inflammation of a human bronchial epithelial cell line (BEAS-2B) and normal human bronchial epithelial (NHBE) cells. Results: Our results showed that expression of miR-181a-2-3p was significantly decreased in Cd-treated cells and silencing of miR-181a-2-3p enhanced Cd-induced inflammatory responses and inflammasome activation. This negative regulatory effect of miR-181a-2-3p on inflammation is partly mediated by the calcium signaling pathway. Furthermore, global gene expression profiling revealed that Toll-like receptor 4 or sequestosome 1 genes were identified as potential targets of miR-181a-2-3p, which were significantly upregulated by knockdown of miR-181a-2-3p in Cd-treated cells. Conclusions: Our results strongly suggest that miR-181a-2-3p has a critical role in Cd-induced inflammation of airway by regulating its potential target genes, which could be molecular targets for COPD.

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Cadmium-induced ER stress and inflammation are mediated through C/EBP–DDIT3 signaling in human bronchial epithelial cells

Cited by 36 publications

References 39 publications

Effect of crocin on PKHD1 and KLLN genes expression in kidney tissue of female rats treated with cadmium

Effect of crocin on PKHD1 and KLLN genes expression in kidney tissue of female rats treated with cadmium

Domesticated gag Gene of Drosophila LTR Retrotransposons Is Involved in Response to Oxidative Stress

Role of miRNA-181a-2-3p in cadmium-induced inflammatory responses of human bronchial epithelial cells

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