Cadmium-induced early changes in O2 •−, H2O2 and antioxidative enzymes in soybean (Glycine max L.) leaves

Muñoz, Nacira; González, Cristian; Molina, Alicia S.; Zirulnik, Fanny; Luna, Claudia Verónica

doi:10.1007/s10725-008-9297-0

Cited by 20 publications

(13 citation statements)

References 34 publications

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“…However, cadmium is not a redox metal and consequently, unlike other redox metals such as Fe, Cu, Zn, etc., it is not able to directly generate ROS through the Haber–Weiss reaction. Thus, cadmium is likely to cause oxidative stress by indirect mechanism such as: the Cd‐induced inhibition of antioxidative enzymes as the CAT (Muñoz et al 2008, Romero‐Puertas et al 2007), APX (Muñoz et al 2008), GR (Schroder et al 2009) or SOD, which could partially due to a Ca deficiency leading to the down‐regulation of CuZn‐SOD (Rodríguez‐Serrano et al 2009); the induction of different peroxidases and oxidases (Azpilicueta et al 2007, Romero‐Puertas et al 2004); the disruption of the electron transport chain (Romero‐Puertas et al 2004) or by inducing lipid peroxidation related to Cd‐mediated lipoxygenase activation (Chaoui et al 1997). Cadmium stress also provokes stomatal closure causing impaired CO 2 assimilation (Gouia et al 2003); this results in limited generation of NADP + , which in turn eases electron transfer from photosystem I to oxygen to form superoxide radical and, subsequently, H 2 O 2 and all its derivates (Liu et al 2007).…”

Section: Discussionmentioning

confidence: 99%

Cadmium-induced oxidative stress and the response of the antioxidative defense system inSpartina densiflora

Domínguez

García

Canalejo

et al. 2010

Physiologia Plantarum

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Spartina densiflora is an invasive cordgrass that is colonizing new habitats and ousting indigenous species in pro-oxidative environments like cadmium-polluted salt marshes in the Odiel estuary (Spain). The aim of our study was to characterize its antioxidative system in order to find out if the system underlies the tolerance of S. densiflora to cadmium toxicity. S. densiflora plants were firstly evaluated to ascertain its antioxidative status in the natural habitat and then they were cultured in the laboratory in unpolluted sand for 28 days. Throughout this period, plants acclimatized and oxidative stress markers reached stable low levels. Then, S. densiflora plants were exposed to cadmium concentrations (10, 100 and 1000 microM Cd) for another 28 days. Higher Cd content in leaves was related to higher level of reactive oxygen species (ROS) causing important oxidative cell damage (lipid peroxidation and lower chlorophyll content). However, S. densiflora possesses a well-organized and appropriately modulated antioxidative defense system which comprises enzymatic activities of guaiacol peroxidase (EC 1.11.1.7), catalase (EC 1.11.1.6), ascorbate peroxidase (EC 1.11.1.11) and superoxide dismutase (EC 1.15.1.1) coupled with the activation of the ascorbate cycle, including enzymatic activities of glutathione reductase (EC 1.6.4.2), dehydroascorbate reductase (EC 1.8.5.1) and monodehydroascorbate reductase (EC 1.6.5.4). This activation was sufficient to reduce Cd-induced ROS accumulation and oxidative damage caused by the lowest Cd-concentrations, but not by the highest Cd-concentration (1000 microM). Nevertheless, the antioxidant system seems to be efficient to achieve a tolerance to cadmium toxicity, allowing normal plant development, even at the presence of highest Cd concentration.

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Section: Discussionmentioning

confidence: 99%

Cadmium-induced oxidative stress and the response of the antioxidative defense system inSpartina densiflora

Domínguez

García

Canalejo

et al. 2010

Physiologia Plantarum

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“…The tubes were vortexed (30 s), incubated (1 h, room temperature), and centrifuged (2,600 × g, 5 min). Supernatant (250 µL (Muñoz et al, 2008;Prasad et al, 2005;Yan et al, 2007) or in soil (Weisany et al, 2012;Zhao et al, 2010). However, plant tissue ROS as H 2 O 2 equivalents can vary by 1000-fold or more (Demidchik, 2015;Queval et al, 2008), depending on growth conditions, plant type (Cheeseman, 2006) and plant growth stage (Cheeseman, 2009).…”

Section: Leaf Ros and Lipid Peroxidation Measurementsmentioning

confidence: 99%

Damage assessment for soybean cultivated in soil with either CeO2 or ZnO manufactured nanomaterials

Priester

Moritz

Espinosa

et al. 2017

Science of The Total Environment

115

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With increasing use, manufactured nanomaterials (MNMs) may enter soils and impact agriculture. Herein, soybean (Glycine max) was grown in soil amended with either nano-CeO (0.1, 0.5, or 1.0gkg soil) or nano-ZnO (0.05, 0.1, or 0.5gkg soil). Leaf chlorosis, necrosis, and photosystem II (PSII) quantum efficiency were monitored during plant growth. Seed protein and protein carbonyl, plus leaf chlorophyll, reactive oxygen species (ROS), lipid peroxidation, and genotoxicity were measured for plants at harvest. Neither PSII quantum efficiency, seed protein, nor protein carbonyl indicated negative MNM effects. However, increased ROS, lipid peroxidation, and visible damage, along with decreased total chlorophyll concentrations, were observed for soybean leaves in the nano-CeO treatments. These effects correlated to aboveground leaf, pod, and stem production, and to root nodule N fixation potential. Soybeans grown in soil amended with nano-ZnO maintained growth, yield, and N fixation potential similarly to the controls, without increased leaf ROS or lipid peroxidation. Leaf damage was observed for the nano-ZnO treatments, and genotoxicity appeared for the highest nano-ZnO treatment, but only for one plant. Total chlorophyll concentrations decreased with increasing leaf Zn concentration, which was attributable to zinc complexes-not nano-ZnO-in the leaves. Overall, nano-ZnO and nano-CeO amended to soils differentially triggered aboveground soybean leaf stress and damage. However, the consequences of leaf stress and damage to N fixation, plant growth, and yield were only observed for nano-CeO.

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“…This is because the activity of superoxide dismutase (SOD) in rice plants was induced more markedly in the root than in the shoot. However, the induction of SOD activity on Cd treatment associated with the stabilization of the system involved in H 2 O 2 removals, such as the lack of ascorbate peroxidase (APX) responses to a stress burst (Olmos et al 2003), resulted in H 2 O 2 accumulation in cell cultures (Muñoz et al 2008). The previous studies' ndings and current information about physiological responses to Cd stress are still not enough to clarify regulation mechanisms for Cd tolerance in rice plants.…”

Section: Introductionmentioning

confidence: 99%

Aspects of cultivar variation in physiological traits related to Cd distribution in rice plants with a short-term stress

Chiao¹,

Chen

Syu

et al. 2020

Preprint

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Background Genotypic variations are seen in cadmium (Cd) tolerance and accumulation in rice plants. Cultivars that show low Cd translocation from the root into shoot can be selected to reduce Cd contamination in rice grains. This study aims to clarify the physiological regulation related to Cd absorption by rice plants for screening out the cultivars, which have relatively low Cd accumulation in grains. Eight Taiwan mega cultivars of paddy rice: japonica (TY3, TK9, TNG71, and KH145 cultivars), indica (TCS10 and TCS17 cultivars), and glutinous (TKW1 and TKW3 cultivars), which are qualified with the criteria for rice grain quality by the Council of Agriculture, Taiwan, were used for illustration. An experiment in hydroponics was conducted for the rice seedlings with a treatment of 50 μM CdCl 2 for 7 days. Results and discussion After the Cd treatment, the reductions in shoot growth were more significant than those in root growth; however, Cd absorbed in the rice plant was sequestered much more in the root. The malondialdehyde (MDA) was preferentially accumulated in rice root but the hydrogen peroxide (H 2 O 2 ) was increased more significantly in the shoot; the antioxidative enzymes, superoxide dismutase (SOD) and ascorbate peroxidase (APX), were pronounced more in rice shoot. Conclusions The rice cultivars preferentially accumulated Cd in the root rather than the shoot with the Cd treatment, which resulted in significant enhancements of MDA and growth reductions in the root. However, H 2 O 2 accumulation was toward the shoot to retard shoot growth suddenly and then the root could keep a gradual growth. Also, the rice cultivars, which preferentially accumulate Cd in the root, would have the regulation tendency of SOD toward the shoot. Due to that SOD is responsible for H 2 O 2 production, H 2 O 2 accumulation would be thus toward the shoot. Moreover, the cultivars, which have a less regulation tendency of APX toward the shoot, would present higher translocation of Cd into the shoot.

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Cadmium-induced early changes in O2 •−, H2O2 and antioxidative enzymes in soybean (Glycine max L.) leaves

Cited by 20 publications

References 34 publications

Cadmium-induced oxidative stress and the response of the antioxidative defense system inSpartina densiflora

Cadmium-induced oxidative stress and the response of the antioxidative defense system inSpartina densiflora

Damage assessment for soybean cultivated in soil with either CeO2 or ZnO manufactured nanomaterials

Aspects of cultivar variation in physiological traits related to Cd distribution in rice plants with a short-term stress

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