Cadmium-induced changes in hatchability and in the activity of aminotransaminases and selected lysosomal hydrolases in the blood plasma of Muscovy ducklings (Cairina moschata)

Abstract

The aim of this study was to determine the effect of cadmium on Muscovy ducklings (Cairina moschata) based on hatching results and the activity of enzymes in the blood plasma. On day 6 of incubation, hatching eggs were injected into the egg albumen with 50 µl of saline solution containing Cd ions (CdCl 2 ) at concentrations of 0 (control group), 1.3, 4.0, 7.5, 15.0 and 30 µg/egg, using 50 eggs per group. A gradual decrease in hatchability, from 52% in the control to 4% in the highest Cd dose group, was observed, with the LD 50 calculated as 8 μg/egg. However, the impact of cadmium on the incidence of malformations of duck embryos has not been proven. Compared to the control group, N-acetyl-β-Dglucosaminidase activity increased by 30-50% (P ≤ 0.05) in the blood serum of ducklings in the groups receiving more than 7.5 µg Cd/egg, whereas an elevated activity of arylsulphatase (by 45%) was observed for a lower dose only (4 µg Cd/egg). A gradual increase in the activity of alanine and aspartate aminotransferases was observed (P ≤ 0.05), starting from the lowest exposure of 1.3 µg Cd/egg, by 155% and 53%, respectively. In conclusion, the results prove the dosedependent toxic impact of cadmium on embryogenesis and on the studied blood plasma enzyme activities of ducklings.

Protection Against Cadmium-Induced Abnormalities and Hepatotoxicity in ovo by Allium sativum

“…Among 11 differentially expressed genes (DEGs) in chicken, which are linked with beak deformity in addition to biosynthesis of unsaturated fatty acids and glycerolipid metabolism (Bai et al, 2014), over-expression of LOC426217 in the beak is thought to be the actual responsible (Bai et al, 2016). Cadmium is found to induce delayed hatching in this study as also reported by Dzugan and Lis (2016).…”

Potential Health Effects of Heavy Metals and Carcinogenic Health Risk Estimation of Pb and Cd Contaminated Eggs from a Closed Gold Mine Area in Northern Thailand

“…Williams et al (2017) reported that, after Pb contamination in birds, the weight and length of bird eggs were significantly decreased, whereas lesions to the liver, kidney, spleen, and thymus were increased [ 53 ]. In terms of Cd, the LD 50 of duck and chicken embryos was 8 μg, besides which they experienced a decrease in hatchability and hepatocyte damage [ 54 , 55 ]. The average concentrations of Cd in this study ranged between 8.94 and 13.13 μg kg −1 , which are also considered harmful levels to poultry in both areas.…”

Searching for Differences in Chemical Composition and Biological Activity of Crude Drone Brood and Royal Jelly Useful for Their Authentication

“…However, different enzymatic profiles for both materials were found. Drone brood showed high hexosaminidase activity (22 times higher than RJ) which is typical for tissue homogenates [ 39 , 40 ]. The most active were α-glucosidase, α-mannosidase and β-galactosidase specific for tissue extracts.…”