Cadmium-Induced Apoptosis in H9c2, A7r5, and C6-Glial Cell

Choi, M. K.; Kim, B. H.; Chung, Yuheon; Han, Moonsik

doi:10.1007/s00128-002-0067-9

Cited by 15 publications

(2 citation statements)

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“…Cadmium is known to induce apoptosis in human T cells, mouse thymocytes, human mononuclear cells, kidney cell lines, myocardial cells, smooth muscle cells, mouse liver cells and C6 glioma cells [46][47][48][49][50]. The susceptibility to cadmium-induced apoptosis is dependent on basal or induced level of MT, which binds cadmium to prevent toxic damage.…”

Section: Apoptosismentioning

confidence: 98%

Metals and apoptosis: Recent developments

Rana

2008

Journal of Trace Elements in Medicine and Biology

311

160

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Section: Apoptosismentioning

confidence: 98%

Metals and apoptosis: Recent developments

Rana

2008

Journal of Trace Elements in Medicine and Biology

311

160

View full text Add to dashboard Cite

“…Apoptosis is a tightly controlled process in which cell death is executed through the activation of specific signaling pathways via many regulatory proteins such as caspases, Bax, Bcl-2, and PKC [17]. Many heavy metals like arsenic, cadmium, and chromium can also induce apoptosis in many cells, including myocardial muscle cells, with varied mechanisms [17][18][19].…”

Section: Introductionmentioning

confidence: 99%

Manganese‐induced apoptosis in rat myocytes

Yang

Sun

Zheng

2007

J Biochem & Molecular Tox

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Manganese can be toxic to the heart, causing dysfunction following long exposure. In our experiments, we examined the cytotoxicity of manganese in neonatal rat ventricular myocytes (NRVM) by MTT assays in vitro. Results showed that after incubation in the different concentrations of manganese for 24 h, apparent cytotoxicity was observed. At 500, 1000, and 1500 2 microM of manganese, the percentage of cell viability dropped to 82% +/- 6.13, 78% +/- 5.28, and 66% +/- 4.22, respectively. When cells were treated for 48 h, all concentrations tested exerted toxic effect; especially from 500 to 1500 microM the cell viability dropped from 67% +/- 4.84 to 37% +/- 3.25. Apoptosis in NRVM was then examined by flow cytometry. Results showed that the percentage of apoptotic cells treated with 500 microM of manganese for 24 h increased from 4% +/- 0.84 to 7% +/- 1.16. After 48 h of incubation, this percentage increased to 11% +/- 0.91. There was no significant difference between control groups (0 microM manganese) after 24 and 48 h incubation. The morphological changes of NRVM nuclei were visualized with the fluorescent DNA-binding dye Hoechst33342 after incubation in 500 microM of manganese for 48 h. Compared with normal nuclei, apoptotic nuclei showed the typical features of fragmentation and condensation. To investigate whether there are any apoptotic gene expression changes during apoptosis, we examined the expression level of Bcl-2, Bax, and P53 mRNAs after treatment with 500 microM of manganese for 48 h. The Bcl-2 mRNA expression decreased while the expression of Bax as well as P53 mRNAs increased. These results suggested that manganese cytotoxicity on NRVM could induce apoptosis in NRVM cells. The apoptosis process might involve, and be promoted by, the changes of the expression levels of P53, Bcl-2, and Bax proteins.

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In vitro cytotoxic assessment of e-waste-related chemical pollution in impacted soil matrix

Eze

Ugochukwu

et al. 2022

Environ Monit Assess

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Cadmium-Induced Apoptosis in H9c2, A7r5, and C6-Glial Cell

Cited by 15 publications

References 0 publications

Metals and apoptosis: Recent developments

Metals and apoptosis: Recent developments

Manganese‐induced apoptosis in rat myocytes

In vitro cytotoxic assessment of e-waste-related chemical pollution in impacted soil matrix

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