Cadmium in the hyperaccumulating mushroom Thelephora penicillata: Intracellular speciation and isotopic composition

Translocation of elements and fractionation of Mg, Cu, Zn, and Cd stable isotopes in a penny bun mushroom (Boletus edulis) from western Czech Republic

“…2d; Table 4). Such a scheme of fractionation is consistent with the data available for some vascular plants (Wei et al 2016;Imseng et al 2019;Zhou et al 2020;Wiggenhauser et al 2021), with the data on Cd isotopic behavior in the system substrate to Thelephora penicillata mushroom (Borovicka et al 2022), and with observations that biochemical processes in general favor initial incorporation of the lighter isotopes in products mobilized from substrates (Shearer and Kohl 1986;Hobbie and Högberg 2012;Jouvin et al 2012;Ryan et al 2013;Fahad et al 2016). The oxidation state of Cd at the environmental conditions is Cd 2+ , and Cd displays chemical properties similar to those of Zn 2+ (Thornton 1986).…”

“…Additionally, Borovicka et al (2022) showed that Cd isotopes may insignificantly fractionate during within-mushroom translocation. Magnesium, Cu and Zn are bioessential elements necessary for most living organisms, whereas Cd is a very toxic and carcinogenic metal which is among the most effectively accumulating elements (e.g., Kaspari and Powers 2016;Gadd 2017;Nordberg et al 2018;Borovicka et al 2022;Filipiak and Filipiak 2022). We conducted the detailed study of trace element distributions and isotope fractionation of Mg, Cu, Zn, and Cd on the growing substrate to mushroom interface, and within the mushroom's fruiting body (penny bun mushroom; Boletus edulis).…”

“…Mushrooms concentrate nutrients and minerals from the substrate (organic matter, soil, rocks) through the long aggregated and branching threads named hyphae (Burford et al 2003 ; Martino et al 2003 ; Fomina et al 2004 ; Gadd 2007 ). Although multiple research has been carried out on trace elements (mainly heavy metals) in mushrooms as possible markers of environmental pollution (Kalac and Svoboda 2000 ; Kalac 2009 and references therein), there is only a limited number of isotopic study of transition metals in biological systems (potentially important in biogeochemical studies) (e.g., Weiss et al 2005 ; Moynier et al 2009 ; Ryan et al 2013 ; Fahad et al 2016 ; Uhlig et al 2017 ; Kříbek et al 2020 ; Borovicka et al 2022 ; Wiggenhauser et al 2022 ).…”

“…Some non-traditional stable isotopes (Mg, Ca, Cu, Zn) have shown fractionation within vascular plants (e.g., Weiss et al 2005 ; Moynier et al 2009 ; Bolou-Bi et al 2010 , 2012 ; Weinstein et al 2011 ; Ryan et al 2013 ; Kříbek et al 2020 ; Wiggenhauser et al 2022 ). However, to the best of our knowledge, only a limited number of non-traditional stable isotope studies was conducted up to date for fungi (Fahad et al 2016 ; Pokharel et al 2017 , 2018 ; Andronikov et al 2022 ; Borovicka et al 2022 ).…”

“…In the frame of our study of mushrooms in the Czech Republic, we figured out that non-traditional stable isotopes (Cu, Zn, and Mg) fractionate both on the substrate-to-mushroom interface and within the mushroom’s fruiting body (Andronikov et al 2022 ). Additionally, Borovicka et al ( 2022 ) showed that Cd isotopes may insignificantly fractionate during within-mushroom translocation. Magnesium, Cu and Zn are bioessential elements necessary for most living organisms, whereas Cd is a very toxic and carcinogenic metal which is among the most effectively accumulating elements (e.g., Kaspari and Powers 2016 ; Gadd 2017 ; Nordberg et al 2018 ; Borovicka et al 2022 ; Filipiak and Filipiak 2022 ).…”