Cadmium activates Arabidopsis MPK3 and MPK6 via accumulation of reactive oxygen species

Liu, Xiaomin; Kim, Kyung Eun; Kim, Kang-Chang; Nguyen, Xuan Canh; Han, Hay Ju; Jung, Myun Sook; Kim, Ho Soo; Kim, Sun Ho; Park, Hyeong Cheol; Yun, Dae‐Jin; Chung, Woo Sik

doi:10.1016/j.phytochem.2010.01.005

Cited by 159 publications

(88 citation statements)

References 37 publications

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“…24 Recently, MPK3 and MPK6 are also evidenced to be activated by Cd-induced ROS accumulation in Arabidopsis plants. 25 A similar effect is also shown in plant response to Al stress, in which a MAPKlike protein is modulated by toxic concentrations of aluminum. 26 …”

Section: Interactions Between Ros and Auxin Signaling In Plant Responmentioning

confidence: 65%

“…It has been documented that mitogen-activated protein kinases (MAPKs) can perceive the changes of ROS content in signal transmission for a range of stresses 23 and the distinct MAPK pathways involve specifically to excess particular metal ions. [24][25][26] ROS induced by Cu triggers SIMK and SAMK activation via SIMKK, whereas Cd activates MMK2, MMK3 besides SIMK and SAMK. 24 Recently, MPK3 and MPK6 are also evidenced to be activated by Cd-induced ROS accumulation in Arabidopsis plants.…”

Section: Interactions Between Ros and Auxin Signaling In Plant Responmentioning

confidence: 99%

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Role of ROS and auxin in plant response to metal-mediated stress

Yuan

Liu

Jin

et al. 2013

Plant Signaling & Behavior

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Section: Interactions Between Ros and Auxin Signaling In Plant Responmentioning

confidence: 65%

Section: Interactions Between Ros and Auxin Signaling In Plant Responmentioning

confidence: 99%

Role of ROS and auxin in plant response to metal-mediated stress

Yuan

Liu

Jin

et al. 2013

Plant Signaling & Behavior

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“…In Arabidopsis, the MAP kinases MPK3 and MPK6 were implicated in ROS signaling (Liu et al, 2010). Therefore, we examined whether HSFA4A can be a substrate for the ROS-stimulated MPK3 and MPK6 through their immunoprecipitation from wild-type plant protein extracts followed by in-gel kinase assays with purified HSFA4A.…”

Section: Hsfa4a Interacts With and Is Phosphorylated By Mpk3 And Mpk6mentioning

confidence: 99%

The Heat Shock Factor A4A Confers Salt Tolerance and Is Regulated by Oxidative Stress and the Mitogen-Activated Protein Kinases MPK3 and MPK6

et al. 2014

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Heat shock factors (HSFs) are principal regulators of plant responses to several abiotic stresses. Here, we show that estradioldependent induction of HSFA4A confers enhanced tolerance to salt and oxidative agents, whereas inactivation of HSFA4A results in hypersensitivity to salt stress in Arabidopsis (Arabidopsis thaliana). Estradiol induction of HSFA4A in transgenic plants decreases, while the knockout hsfa4a mutation elevates hydrogen peroxide accumulation and lipid peroxidation. Overexpression of HSFA4A alters the transcription of a large set of genes regulated by oxidative stress. In yeast (Saccharomyces cerevisiae) two-hybrid and bimolecular fluorescence complementation assays, HSFA4A shows homomeric interaction, which is reduced by alanine replacement of three conserved cysteine residues. HSFA4A interacts with mitogen-activated protein kinases MPK3 and MPK6 in yeast and plant cells. MPK3 and MPK6 phosphorylate HSFA4A in vitro on three distinct sites, serine-309 being the major phosphorylation site. Activation of the MPK3 and MPK6 mitogen-activated protein kinase pathway led to the transcriptional activation of the HEAT SHOCK PROTEIN17.6A gene. In agreement that mutation of serine-309 to alanine strongly diminished phosphorylation of HSFA4A, it also strongly reduced the transcriptional activation of HEAT SHOCK PROTEIN17.6A. These data suggest that HSFA4A is a substrate of the MPK3/MPK6 signaling and that it regulates stress responses in Arabidopsis.

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“…Cadmium has adverse effects on plant development such as inhibition of root growth and induction of chlorosis in leaves. 9 In Arabidopsis, it was reported that HIPP20, HIPP22, HIPP26, and HIPP27 played an important role in Cd-detoxification. 8 In order to determine whether UBP16 is involved in Cadmium tolerance, we transferred 7-d-old seedlings grown on MS medium to MS medium with or without 30 μM CdCl 2 for 12 d. In the absence of CdCl 2 , there was no significant difference in shoot growth and anthocynanin contents between wild-type and the ubp16 mutant seedlings.…”

Section: Ubiquitin-specificmentioning

confidence: 99%

UBIQUITIN-SPECIFIC PROTEASE16 interacts with a HEAVY METAL ASSOCIATED ISOPRENYLATED PLANT PROTEIN27 and modulates cadmium tolerance

Zhao

Zhou

2013

Plant Signaling & Behavior

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Protein ubiquitination and deubiquitination are two reversible processes catalyzed by ubiquitin ligases and deubiquitinating enzymes, respectively. in Arabidopsis, lots of substrates of ubiquitin ligases were found, whereas only a few targets of deubiquitinating enzymes were identified. recently, we reported that a functional uBiQuitin-SPECiFiC ProtEaSE16 (uBP16) was involved in salt tolerance through positively regulating plasma membrane na + /h + antiport activity and at least partially modulating SErinE hYDroXYmEthYLtranSFEraSE1 (Shm1) stability and activity. here, we report that uBP16 interacts with hEaVY mEtaL aSSoCiatED iSoPrEnYLatED PLant ProtEin27 (hiPP27), a metallochaperone containing a predicted heavy-metal-associated domain, which has been reported to play an important role in cadmium detoxification. meanwhile, the ubp16 mutant showed more sensitive to cadmium than wild-type. taken together, hiPP27 may be another target of uBP16 in cadmium response.

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Cadmium activates Arabidopsis MPK3 and MPK6 via accumulation of reactive oxygen species

Cited by 159 publications

References 37 publications

Role of ROS and auxin in plant response to metal-mediated stress

Role of ROS and auxin in plant response to metal-mediated stress

The Heat Shock Factor A4A Confers Salt Tolerance and Is Regulated by Oxidative Stress and the Mitogen-Activated Protein Kinases MPK3 and MPK6

UBIQUITIN-SPECIFIC PROTEASE16 interacts with a HEAVY METAL ASSOCIATED ISOPRENYLATED PLANT PROTEIN27 and modulates cadmium tolerance

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