Cadmium accumulation, distribution and elimination in the bivalve Macoma balthica: Neither metallothionein nor metallothionein-like proteins are involved

Langston, W.J.; Zhou, Mingjiang

doi:10.1016/0141-1136(87)90067-5

Cited by 63 publications

(24 citation statements)

References 13 publications

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“…Littorina littorea, Langston & Zhou 1987;Biomphalaria glabrata, Guerrero et al 2000), but Cd was redistributed among different tissues in the animals during the depuration period (Langston & Zhou 1987, Guerrero et al 2000. Langston & Zhou (1987) could not detect any loss of Cd from L. littorea during a 7 mo depuration period.…”

Section: Transfer From Phytoplankton To Filter-feeding Bivalves and Pmentioning

confidence: 97%

“…Cd and Zn k e s in 2 gastropods (Nassarius teretiusculus and Babylonia formasae habei) were as low as 0.00113 to 0.0055 d ). Again, the long retention of metals in the gastropods may be related to the binding of Cd with MTs in phosphate granules in the digestive glands, which can effectively sequester metals (Nott & Langston 1989, Langston & Zhou 1987. Similar binding resulting in an extremely low efflux has been documented in barnacles , Rainbow & Wang 2001.…”

Section: Transfer From Phytoplankton To Filter-feeding Bivalves and Pmentioning

confidence: 99%

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Interactions of trace metals and different marine food chains

Wang¹

2002

Mar. Ecol. Prog. Ser.

481

268

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There is increasing recognition of the quantitative importance of metal accumulation from dietary pathways in different marine food webs. With a simple kinetic model requiring measurements of metal assimilation efficiency (AE), metal efflux rate and ingestion activity of the relevant animals, it is possible to predict the transfer (and potential biomagnification) of metals along diverse marine food chains. In this paper, food chain transfer in different marine food chains (planktonic and benthic) is reviewed, and any potential biomagnification of metals is predicted using the simple kinetic equation. It is well recognized that metal concentrations in animals are probably not related to the trophic level in the food chain, but some metals (Hg and Zn) may potentially be influenced by the numbers of trophic interactions involved. Depending on these different trophic interactions, the potential biomagnification of metals is influenced by metal assimilation or metal efflux rate or both. Metals may potentially be biomagnified in benthic food chains; principally, in those with gastropods as top predators, as they can efficiently sequester dietary metals. The biodiminution of metals (concentration decreases at a higher trophic level) in the classical marine planktonic food chain (phytoplankton to copepods to fish) is largely the result of the effective efflux of metals by the copepods and the very low assimilation of metals by marine fish. Although many past studies have considered the physico-chemical properties of metals in prey in controlling food chain transfer, it is suggested that the physiological and biochemical processes in the herbivore or predator should be emphasized in future studies. Also, the complicated metal-handling strategies found in different marine organisms clearly need to be appreciated. KEY WORDS: Food chain · Trace metals · Biomagnification · Trophic transfer factor · Invertebrates · FishResale or republication not permitted without written consent of the publisher

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Section: Transfer From Phytoplankton To Filter-feeding Bivalves and Pmentioning

confidence: 97%

Section: Transfer From Phytoplankton To Filter-feeding Bivalves and Pmentioning

confidence: 99%

Interactions of trace metals and different marine food chains

Wang¹

2002

Mar. Ecol. Prog. Ser.

481

268

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“…Dissolved metals may also be incorporated from either the lumen of the diverticula or from blood at the cell bottom. The dissolved metals are first immobilized by cytosolic metal-binding proteins (MT and HMWP) and further engulfed into lysosomes Simkiss and Mason, 1984;Fowler, 1987;Langston and Zhou, 1987). The cellular distribution of the metal may vary greatly depending on the metal retention characteristics of the lysosomes , often related to the nature of the mechanisms of formation and accumulation of lipofuscins (Viarengo, 1989), which may vary between species, according to the particularities of the digestion process.…”

Section: Cells Binding Metals By Sulfur Donorsmentioning

confidence: 99%

Cellular and subcellular distribution of metals in molluscs

Marigómez

Soto

Cajaraville

et al. 2002

Microscopy Res & Technique

419

202

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The cellular processes involved in metal metabolism in molluscs are reviewed, with emphasis on the contribution of microscopy (AMG, ARG, EPMA, and SIMS) to both basic research of metal cell biology and applied environmental research. In molluscs, metal uptake may occur by facilitated diffusion, active transport, or endocytosis, and can be enhanced by MT synthesis or formation of mineralized granules. In aquatic molluscs, gills constitute a key interface for dissolved metal uptake, where metals are bound to MT, incorporated into lysosomes, and released basally towards the blood plasma and circulating hemocytes. However, particulate metal uptake is mainly achieved via the digestive tract by endocytosis; further metals are transferred first to lysosomes and then to residual bodies, especially in the digestive cells of the digestive gland. Additionally, metals can be accumulated selectively in specific cell types. As ligands pools differ from cell to cell, different metals may be retained in different cell types. Class "a" metals are localized in cells with granules composed of carbonate, oxalate, phosphate, and sulfate (oxygen donors), whereas "b" metals are associated with those cell types rich in sulfur and nitrogen ligands (sulfur donors). In molluscs, oxygen donors occur in connective tissue calcium cells and basophilic cells, whereas sulfur donors are present in digestive cells, podocytes, nephrocytes, and rhogocytes. Hemocytes, which constitute the most relevant system for metal transport between tissues, move around the body and may penetrate tissues and remove metals from the inner medium to be accumulated in lysosomes as nondigested products. Rhogocytes also participate in metal mobilization, accumulation, and release. The assessment of metal levels in target cells of sentinel molluscs by microscopic techniques provides an early-warning measure, with promising applications as an exposure biomarker for environmental monitoring programs.

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“…Thus, MTs seem to play only a minor role in metal sequestration in O. edulis. Limited or negligible involvement of MTs in metal-binding has been also reported for other bivalves such as Anodonta cygnea and Unio elongatulus or Macoma balthica (Langston & Zhou 1987), confirming that metal exposure does not always result in detectable induction, and this suggests that the involvement of MTs in regulation and detoxification is not always consistent. Previous works have also shown that in acute cadmium exposure experiments, Crassostrea virginica presented different cytosolic distribution of cadmium, with a large percentage of the cytosolic cadmium occurring in the very low molecular weight material, < 5 kDa fraction, rather than being associated with the MT complex (Ruddel & Rains 1975, Simkiss & Taylor 1989, Engel 1999.…”

Section: Discussionmentioning

confidence: 73%

Metallothionein genes in the European flat oyster Ostrea edulis: a potential ecological tool for environmental monitoring?

Tanguy¹,

Boutet²,

Riso³

et al. 2003

Mar. Ecol. Prog. Ser.

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Metallothioneins (MTs) are small essential proteins involved in cellular processes of metal handling and detoxification. Here, we describe the structure of 2 MT genes in a marine mollusc, the European flat oyster Ostrea edulis. The sequences of these 2 genes code for 2 proteins of 74 and 71 amino acids, respectively, with the 71 amino acid protein having an abnormal lack of cysteine residues. The direct quantification of metal ions bound to purified recombinant proteins demonstrated that the metal-binding capacity of the 2 proteins differs by about 20%. An enzyme-linked immunosorbent assay (ELISA) test, used to quantify MT protein expression in O. edulis exposed to metal under laboratory conditions, showed no significant induction of MTs, either in the gills or the digestive gland despite an increase in metal concentration observed in the same tissues. Similar results were observed with an MT-RNA expression study. These results seem to indicate a low involvement of MTs in metal detoxification in O. edulis. Examination of polymorphism in the coding sequence of OeMT (O. edulis metallothionein) genes by single-strand conformation polymorphism (SSCP) revealed a lack of genetic diversity in field populations. KEY WORDS: Metallothionein · Detoxification · Expression · ELISA · Polymorphism · Ostrea edulisResale or republication not permitted without written consent of the publisher

show abstract

Cadmium accumulation, distribution and elimination in the bivalve Macoma balthica: Neither metallothionein nor metallothionein-like proteins are involved

Cited by 63 publications

References 13 publications

Interactions of trace metals and different marine food chains

Interactions of trace metals and different marine food chains

Cellular and subcellular distribution of metals in molluscs

Metallothionein genes in the European flat oyster Ostrea edulis: a potential ecological tool for environmental monitoring?

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