Cadherin interplay during neural crest segregation from the non‐neural ectoderm and neural tube in the early chick embryo

Abstract

Our results indicate that partition of the neuroectoderm is mediated by cadherin interplays and ascribes a key role to Cadherin-6B in the specification and delamination of the NC population. Developmental Dynamics 246:550-565, 2017. © 2017 Wiley Periodicals, Inc.

MMP14 Regulates Cranial Neural Crest Epithelial‐to‐Mesenchymal Transition and Migration

“…There seems to be a partially complementary pattern in the expression of the two cadherins, yet we do not know whether this correlates to cells in the migration front vs. rear or whether one cadherin simply compensate for the dynamic changes of another cadherin. Such differential yet overlapping expression of E-cadherin and N-cadherin is also observed in chick embryos during neural crest cell delamination (Dady and Duband, 2017). When MMP14 was overexpressed, consistent with the previous observations (Figure 5), there were more NCCs detached from the explant to migrate individually or in small cell clusters (small arrows).…”

“…Multiple cadherin proteins have been reported to be dynamically expressed to mediate this process, including neural cadherin (N-cadherin) and epidermal cadherin (E-cadherin) (Akitaya and Bronner-Fraser, 1992; Nakagawa and Takeichi, 1998; Dady et al, 2012; Rogers et al, 2013; Taneyhill and Schiffmacher, 2013; Scarpa et al, 2015; Huang et al, 2016; Dady and Duband, 2017). In frog cranial NCCs, both N-cadherin and E-cadherin are expressed during these critical stages: from premigratory stages, through delamination, and during migration.…”

Neural crest delamination and migration: Looking forward to the next 150 years

“…In chick, the cephalic NC first express E‐cadherin, which is decreased and replaced by N‐cadherin (Rogers, Saxena, & Bronner, ). E‐cadherin is not completely lost, it is still present in the early migratory NC cells (Dady & Duband, ; Lee et al, ). However, this change from E to N‐cadherin is important for completing EMT.…”

Cut loose and run: The complex role of ADAM proteases during neural crest cell development

“…Studies by other groups have established the importance of additional cadherins besides N‐cadherin, such as E‐cadherin and Cadherin‐6B, in premigratory and migratory NC throughout the cranial and trunk axial levels of the chick (Coles, Taneyhill, & Bronner‐Fraser, ; Dady et al, ; Dady & Duband, ; Lee et al, ; Nakagawa & Takeichi, ; Taneyhill, Coles, & Bronner‐Fraser, ). Through immunohistochemical and in situ hybridization analyzes, Dady and Duband (Dady & Duband, ; Dady et al, ) demonstrated that E‐cadherin and Cadherin‐6B are expressed in the proper spatio‐temporal pattern to play a role in the specification of the NC and defining the premigratory NC domain (Dady & Duband, ), including in the head and anterior and mid trunk. Furthermore, these authors demonstrate that Cadherin‐6B expression, but not N‐cadherin, defines the premigratory NC domain in these truncal regions, with delaminating cells here devoid of Cadherin‐6B , N‐cadherin, and E‐cadherin, but later expressing Cadherin‐7 during migration.…”