Cadherin expression in the inner ear of developing zebrafish

Novince, Z. M.; Azodi, E.; Marrs, James A.; Raymond, Pamela A.; Liu, Q.

doi:10.1016/s1567-133x(03)00030-9

Cited by 13 publications

(13 citation statements)

References 15 publications

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“…Synthesis of digoxigenin-labeled cdh1, cdh2, cdh4, and chd11 RNA probes for in situ hybridization was described previously (Liu et al, 1999;Babb et al, 2001;Novince et al, 2003). Detailed procedures for in situ hybridization were reported previously (Liu et al, 1999).…”

Section: Methodsmentioning

confidence: 99%

Cadherin‐1, ‐2, and ‐11 expression and cadherin‐2 function in the pectoral limb bud and fin of the developing zebrafish

Liu¹,

Kerstetter²,

Azodi³

et al. 2003

Developmental Dynamics

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Cadherins are cell adhesion molecules that play important roles in development of a variety of organs, including the vertebrate limb. In this study, we analyze cadherin expression patterns in the embryonic zebrafish pectoral limb buds and larval pectoral fins by using both in situ hybridization and immunocytochemical methods. cadherin-1 is detected in the epidermis of the embryonic limb buds and the larval pectoral fins. Cadherin-2 is expressed in the pectoral limb bud mesenchyme and chondrogenic condensation. As development proceeds, cadherin-2 expression is detected in newly differentiated pectoral fin endoskeleton, but its expression is greatly down-regulated in the fin endoskeleton of larval zebrafish. cadherin-11 is found in the basal region of the embryonic limb buds and in the proximal endoskeleton of the larval pectoral fins. Interfering with cadherin-2 function using two specific antisense morpholino oligonucleotides disrupts formation of the chondrogenic condensation/endoskeleton, suggesting that cadherin-2 is crucial for the normal development of the zebrafish pectoral fins. Developmental Dynamics 228:734 -739, 2003.

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Section: Methodsmentioning

confidence: 99%

Cadherin‐1, ‐2, and ‐11 expression and cadherin‐2 function in the pectoral limb bud and fin of the developing zebrafish

Liu¹,

Kerstetter²,

Azodi³

et al. 2003

Developmental Dynamics

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“…Our previous studies showed that Cdh2/cdh2 is expressed as soon as sensory patches form during inner ear development, and expression increases with patch size (Novince et al, 2003), suggesting that Cdh2 may contribute to the organization of sensory patches or differentiation of hair cells and supporting cells. We tested these ideas by using morpholino knockdown technology and by examining cdh2 null mutant (glo) embryos.…”

Section: Discussionmentioning

confidence: 99%

“…Our previous studies showed that cadherin-2 expression is first seen concomitantly with the formation of sensory patches during inner ear development (Novince et al, 2003), suggesting that Cdh2 may help to regulate the organization of sensory patches or differentiation of hair cells and supporting cells. The effect of cdh2 MO and glo mutation on differentiation of sensory patches was examined by double labeling 2-day-post-fertilization (dpf) embryos with an acetylated tubulin antibody to identify the hair cell kinocilia and Rhodamine-conjugated phalloidin, which in turn identifies hair cell stereocilia.…”

Section: Journal Of Cell Science 119 (24)mentioning

confidence: 98%

“…We reported that Cdh2/cdh2 protein and message are expressed in the developing inner ear, accumulating in the developing sensory patches that give rise to the hair cells and supporting cells (Novince et al, 2003), which suggests that Cdh2 might be involved in the regulation of sensory patch formation. Here we test this hypothesis by examining the effects of cdh2 loss-of-function through application of antisense oligonucleotide (morpholino oligonucleotides; MOs) knockdown experiments (MO-induced phenotype is referred to as morphant phenotype, in contrast to mutant phenotype) and by studying aspects of ear development in the cdh2 mutant, glass onion (glo).…”

Section: Introductionmentioning

confidence: 95%

“…The zebrafish has become an important genetic model organism, which is amenable to genetic analysis of ear development (Whitfield et al, 1996;Ernest et al, 2000;Whitfield et al, 2002;Liu et al, 2003;Nicolson, 2005), including the role of cadherin-23 in mechanotransduction of sound (Sollner et al, 2004;Nicolson, 2005). We have initiated a series of studies to evaluate the contribution of cadherin cellcell adhesion function to zebrafish inner ear development (Novince et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

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Cadherin-2 participates in the morphogenesis of the zebrafish inner ear

Clendenon

Shen

Liu

et al. 2006

Journal of Cell Science

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Molecular mechanisms that control inner ear morphogenesis from the placode to the three-dimensional functional organ are not well understood. We hypothesize that cell-cell adhesion, mediated by cadherin molecules, contributes significantly to various stages of inner ear formation. Cadherin-2 (Cdh2) function during otic vesicle morphogenesis was investigated by examining morpholino antisense oligonucleotide knockdown and glass onion (glo) (Cdh2 mutant) zebrafish embryos. Placode formation, vesicle cavitation and specification occurred normally, but morphogenesis of the otic vesicle was affected by Cdh2 deficiency: semicircular canals were reduced or absent. Phalloidin staining of the hair cell stereocillia demonstrated that cadherin-2 (cdh2) loss-of-function did not affect hair cell number, but acetylated tubulin labeling showed that hair cell kinocilia were shorter and irregularly shaped. Statoacoustic ganglion size was significantly reduced, which suggested that neuron differentiation or maturation was affected. Furthermore, cdh2 loss-of-function did not cause a general developmental delay, since differentiation of other tissues, including eye, proceeded normally. These findings demonstrate that Cdh2 selectively affects epithelial morphogenetic cell movements, particularly semicircular canal formation, during normal ear mophogenesis.

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