Cadherin-dependent differential cell adhesion in <i>Xenopus</i> causes cell sorting in vitro but not in the embryo

Ninomiya, H.; David, Robert; Damm, Erich W.; Fagotto, François; Niessen, Carien; Winklbauer, Rudolf

doi:10.1242/dev.084764

Cited by 18 publications

(32 citation statements)

References 1 publication

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These findings are intriguingly similar to work in Xenopus showing that in the embryo, cadherin morphant cells are able move past both cadherin-overexpressing and cadherin-depleted cells (Ninomiya et al, 2012). In this study, Ninomiya et al (2012) showed that cells can locally up or downregulate cadherin density at the plasma membrane, thereby modulating their adhesiveness. Potentially wild-type cells contacting MZspg cells down-regulate E-cadherin which facilitates cell movement.…”

Section: Developmental Dynamicssupporting

confidence: 88%

Zebrafish epiboly: Spreading thin over the yolk

Bruce

2015

Developmental Dynamics

View full text Add to dashboard Cite

Tissue thinning and spreading, a morphogenetic movement termed epiboly, is used widely during animal development. In zebrafish, epiboly is a prominent cell movement during gastrulation, whereby a squamous epithelium (the enveloping layer), a multi-layer of loosely packed cells (the deep cells), and a yolk nuclear syncytium (the yolk syncytial layer) undergo coordinated expansion to engulf the yolk and close the blastopore. Elucidating the mechanisms that underlie epiboly is important not only for understanding animal development in general, but also for providing insights into fundamental cell behaviors including cell intercalation, cell adhesion, cell signaling, and epithelial morphogenesis. Here, recent work is reviewed with a focus on findings that advance our understanding of (1) the role of actomyosin motors in the yolk cell to drive epiboly, (2) the mechanisms that underlie the spreading of the epithelial enveloping layer, and (3) the regulation of deep cell movements by E-cadherin based adhesion. A discussion of how these new insights add to the current view of epiboly and future prospects is also presented. Overall, the study of zebrafish epiboly can provide general and broadly applicable insights into the genetic, molecular, and cellular control of morphogenesis. Developmental Dynamics 245:244-258, 2016. V C 2015 Wiley Periodicals, Inc.

show abstract

Section: Developmental Dynamicssupporting

confidence: 88%

Zebrafish epiboly: Spreading thin over the yolk

Bruce

2015

Developmental Dynamics

View full text Add to dashboard Cite

show abstract

“…It has also been shown that N-cadherin expression triggers active endodermal cell migration, which leads to the cell segregation and germ layer formation (Ninomiya et al, 2012). Moreover, a cadherin switch allows efficient Wnt, BMP and FGF signaling, required for the proper mesoderm differentiation in fruitfly and mouse (Basilicata et al, 2016;Giger and David, 2017;Ninomiya et al, 2012;Schäfer et al, 2014). In line with that, N-cadherin can interact with the FGF receptor and modulate the signaling pathway (Francavilla et al, 2009;Williams et al, 1994).…”

Section: Introductionmentioning

confidence: 87%

“…During mesoderm formation of Drosophila, DE-cadherin becomes replaced by DN-cadherin (Oda et al, 1998), similar to the switch from E-to N-cadherin during mesoderm formation in chicken (Hatta and Takeichi, 1986). It has also been shown that N-cadherin expression triggers active endodermal cell migration, which leads to the cell segregation and germ layer formation (Ninomiya et al, 2012). Moreover, a cadherin switch allows efficient Wnt, BMP and FGF signaling, required for the proper mesoderm differentiation in fruitfly and mouse (Basilicata et al, 2016;Giger and David, 2017;Ninomiya et al, 2012;Schäfer et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Cadherin switch marks germ layer formation in the diploblastic sea anemone Nematostella vectensis

Pukhlyakova

Kirillova

Kraus

et al. 2018

Preprint

View full text Add to dashboard Cite

Morphogenesis is a shape-building process during development of multicellular organisms. During this process the establishment and modulation of cell-cell contacts play an important role. Cadherins, the major cell adhesion molecules, form adherens junctions connecting ephithelial cells. Numerous studies in Bilateria have shown that cadherins are associated with the regulation of cell differentiation, cell shape changes, cell migration and tissue morphogenesis. To date, the role of Cadherins in nonbilaterians is unknown. Here, we study the expression and the function of two paralogous classical cadherins, cadherin1 and cadherin3, in the diploblastic animal, the sea anemone Nematostella vectensis. We show that a cadherin switch is accompanying the formation of germ layers. Using specific antibodies, we show that both cadherins are localized to adherens junctions at apical and basal positions in ectoderm and endoderm. During gastrulation, partial EMT of endodermal cells is marked by a step-wise downregulation of cadherin3 and upregulation of cadherin1. Knockdown experiments show that both cadherins are required for maintenance of tissue integrity and tissue morphogenesis. This demonstrates that cnidarians convergently use cadherins to differentially control morphogenetic events during development.

show abstract

“…The substratum for PCM cell‐on‐cell migration is not known. Depletion of C‐cadherin or expression of a truncated form has little effect on the movement (Broders & Thiery, ; Ninomiya et al, ). Migration also appears unperturbed after FN knockdown (our unpublished data).…”

Section: Modes Of Cell Migration In the Gastrulamentioning

confidence: 99%

“…At slightly lower levels, involution and convergent extension of CM were affected. However, movements in other gastrula regions, including the LEM, appeared normal, despite cells being less densely packed (Broders & Thiery, ; Kalantarian et al, ; Kühl, Finnemann, Binder, & Wedlich, ; Lee & Gumbiner, ; Ninomiya et al, ).…”

Section: Modes Of Cell Migration In the Gastrulamentioning

confidence: 99%

Cell migration in the Xenopus gastrula

Huang

Winklbauer

2018

WIREs Developmental Biology

Self Cite

View full text Add to dashboard Cite

Xenopus gastrulation movements are in large part based on the rearrangement of cells by differential cell-on-cell migration within multilayered tissues. Different patterns of migration-based cell intercalation drive endoderm and mesoderm internalization and their positioning along their prospective body axes. C-cadherin, fibronectin, integrins, and focal contact components are expressed in all gastrula cells and play putative roles in cell-on-cell migration, but their actual functions in this respect are not yet understood. The gastrula can be subdivided into two motility domains, and in the vegetal, migratory domain, two modes of cell migration are discerned. Vegetal endoderm cells show ingression-type migration, a variant of amoeboid migration characterized by the lack of locomotory protrusions and by macropinocytosis as a mechanism of trailing edge resorption. Mesendoderm and prechordal mesoderm cells use lamellipodia in a mesenchymal mode of migration. Gastrula cell motility can be dissected into traits, such as cell polarity, adhesion, mobility, or protrusive activity, which are controlled separately yet in complex, combinatorial ways. Cells can instantaneously switch between different combinations of traits, showing plasticity as they respond to substratum properties.

show abstract

Cadherin-dependent differential cell adhesion in Xenopus causes cell sorting in vitro but not in the embryo

Cited by 18 publications

References 1 publication

Zebrafish epiboly: Spreading thin over the yolk

Zebrafish epiboly: Spreading thin over the yolk

Cadherin switch marks germ layer formation in the diploblastic sea anemone Nematostella vectensis

Cell migration in the Xenopus gastrula

Contact Info

Product

Resources

About