Cadherin-11 endocytosis through binding to clathrin promotes cadherin-11-mediated migration in prostate cancer cells

Satcher, Robert L.; Pan, Tianhong; Bilen, Mehmet Asım; Li, Xiaoxia; Lee, Yu Chen; Ortiz, Angélica; Kowalczyk, Andrew P.; Yu-Lee, Li-Yuan; Lin, Sue Hwa

doi:10.1242/jcs.176081

Cited by 22 publications

(28 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, the present study observed that the prognostic value may be largely enhanced by co-evaluating the expression of these three proteins in patients with OSCC. These results were in line with those of previous studies in other types of cancer (29)(30)(31), indicating that CDH6, CDH11 and CD44 may be either directly or indirectly involved in the metastasis of OSCC. Therefore, it is reasonable to combine the expression of these three proteins as predictive parameters for improved evaluation of the progression of OSCC.…”

Section: Discussionsupporting

confidence: 92%

“…It is well-known that loss of E-cadherin and increased expression of N-cadherin is associated with tumor cell invasion in oral and ovarian cancer (28,29). By contrast, other mesenchymal-associated cadherins and adhesion factors, including CDH6, CDH11 and CD44, which have been proven to be able to interfere with epithelial cell-cell adhesion and to promote cancer cell invasion and metastasis, are often overexpressed in ovarian cancer (30)(31)(32). Until now, however, the precise role of the three factors in OSCC has not received a great deal of attention.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Combined overexpression of cadherin 6, cadherin 11 and cluster of differentiation 44 is associated with lymph node metastasis and poor prognosis in oral squamous cell carcinoma

Zhao

Lin

et al. 2018

Oncol Lett

View full text Add to dashboard Cite

Oral squamous cell carcinoma (OSCC) is a highly invasive lesion that frequently metastasizes to the cervical lymph nodes and is associated with a poor prognosis. Several adhesion factors, including cadherin 6 (CDH6), cadherin 11 (CDH11) and cluster of differentiation 44 (CD44), have been reported to be involved in the invasion and metastasis of multiple types of cancer. Therefore, the aim of the present study was to determine the expression of CDH6, CDH11 and CD44 in tumor tissues from patients with OSCC, and whether this was associated with the metastasis and survival of OSCC. The mRNA expression of the human tumor metastasis-related cytokines was examined by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) in OSCC tumors with or without lymph node metastasis (n=10/group). The expression of CDH6, CDH11 and CD44 in 101 OSCC and 10 normal oral mucosa samples was examined by immunohistochemical staining. The association between overall and disease-specific survival times of patients with OSCC and the expression of these three proteins was evaluated using Kaplan-Meier curves and the log-rank test. RT-qPCR results indicated that the mRNA expression of CDH6, CDH11 and CD44 was increased in OSCC patients with lymph node metastasis (2.93-, 2.01- and 1.92-fold; P<0.05). Overexpression of CDH6, CDH11 and CD44 was observed in 31/35 (89%), 25/35 (71%) and 31/35 (89%) patients, respectively. The number of OSCC patients with lymph node metastasis exhibiting CDH6, CDH11 and CD44 overexpression was significantly higher than the number of patients without lymph node metastasis exhibiting overexpression of these proteins (P=0.017, P=0.038 and P=0.007, respectively). OSCC patients with high co-expression of CDH6, CDH11 and CD44 exhibited lower disease-specific survival times (P=0.047; χ=3.933) when compared with OSCC patients with low co-expression of these adhesion factors. CDH6, CDH11 and CD44 serve important roles in OSCC metastasis and the combined use of these factors as biomarkers may improve the accuracy of the prediction of cancer metastases and prognosis.

show abstract

Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Combined overexpression of cadherin 6, cadherin 11 and cluster of differentiation 44 is associated with lymph node metastasis and poor prognosis in oral squamous cell carcinoma

Zhao

Lin

et al. 2018

Oncol Lett

View full text Add to dashboard Cite

show abstract

“…The proteomic study of urine in prostate cancer patients with a Gleason score of 6.9 ± 1.1 showed that LXR/RXR is activated and the signal of the acute-phase response is significantly downregulated [84]. Clathrin-mediated endocytosis is associated with the migration of prostate cancer cells [85]. The regulation of Rho on actin movement is significantly enriched after the tumor is palpable.…”

Section: Rm-1 Prostate Cancer-bearing Micementioning

confidence: 99%

Dynamic changes in the urine proteome of tumor-bearing mouse models of B16 melanoma and RM-1 prostate cancer

Pan

Liu

et al. 2020

Preprint

View full text Add to dashboard Cite

Urine can accumulate changes and reflect early physiological and pathological changes of various diseases, such as tumors. Therefore, urine is an ideal source for identification of early biomarkers. In this study, melanoma and prostate cancer-bearing mouse models were established by subcutaneous injection of B16 and RM-1 cells, respectively. Urine samples were collected at four time points during tumor growth. Based on dataindependent acquisition (DIA) technology, liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used for quantitative analysis. Compared with those before the injection of B16 cells, 38 human homologous differential proteins were identified, and 18 proteins were reported to be related to melanoma. Before the tumor was visible, there were 4 differential proteins, and all were reported to be related to melanoma. Compared with that before the injection of RM-1 cells, a total of 14 human homologous differential proteins were identified, and 9 proteins were reported to be associated with prostate cancer. Before the tumor was palpable, 9 proteins showed significant differences. There were significant differences between the two tumor-bearing models. Through the above experiments and analysis, we found that the urine proteome can reflect the changes in the development and provide early biomarkers of the two tumors and provide clues for the early clinical diagnosis of these diseases.Skin melanoma is one of the most common cancers in the world [1]. Melanoma is produced by the malignant transformation of melanocytes. This disease can occur in any part of the skin and mucous membranes. Melanoma easily metastasizes and is highly invasive and malignant, leading to high mortality. The diagnosis of melanoma is based on a combination of clinical and pathological techniques, including physical examination, histopathological examination and imaging examination. However, due to its variable histological morphology, with little or even no visible pigment, it is difficult to diagnose patients in the early stage and distinguish this condition from benign nevus in the clinic, which makes its early diagnosis difficult [2]. Early treatment of melanoma is mainly surgery, and advanced treatment includes chemotherapy, targeted therapy and immunotherapy. Compared with chemotherapy alone, targeted therapy and immunotherapy have significantly improved the survival rate of patients with advanced melanoma, but these two therapies are usually limited by the drug resistance rate and are not universally applicable [3,4]. The absolute survival rate of patients is still very low [5]. The 5-year survival rates of skin melanoma at stage I to IV are 97% (stage IA), 84% (stage IB), 68%, 55%, and 17% [6]. Therefore, early diagnosis can lead to early treatment and improve the survival rate of patients.Prostate cancer is one of the most common malignant tumors in men. In Europe and America, the incidence rate is highest among those of all cancers in males. The mortality rate is second only to that of lung cancer, ranking second fo...

show abstract

“…In addition to the DEE and dileucine motifs, a recent study has exposed a new endocytic motif that is also potentially subject to regulation by p120. A mesenchymal cadherin that is expressed in osteoblasts (cadherin‐11) directly interacts with clathrin through a unique sequence, VFEEE, in the JMD of the cadherin tail . Mutation of this sequence inhibits endocytosis of the cadherin in PC3mm2 cells, a highly metastatic cell prostate tumor cell line .…”

Section: P120: Master Guardian Of Cadherin Endocytosismentioning

confidence: 99%

“…A mesenchymal cadherin that is expressed in osteoblasts (cadherin‐11) directly interacts with clathrin through a unique sequence, VFEEE, in the JMD of the cadherin tail . Mutation of this sequence inhibits endocytosis of the cadherin in PC3mm2 cells, a highly metastatic cell prostate tumor cell line . The VFEEE sequence is similar to the LxEx(D/E) clathrin‐binding box sequence found in the C‐terminal tail of arrestin3 .…”

Section: P120: Master Guardian Of Cadherin Endocytosismentioning

confidence: 99%

Cadherin tales: Regulation of cadherin function by endocytic membrane trafficking

Cadwell

Kowalczyk

2016

Traffic

Self Cite

View full text Add to dashboard Cite

Cadherins are the primary adhesion molecules in adherens junctions and desmosomes and play essential roles in embryonic development. Although significant progress has been made in understanding cadherin structure and function, we lack a clear vision of how cells confer plasticity upon adhesive junctions to allow for cellular rearrangements during development, wound healing and metastasis. Endocytic membrane trafficking has emerged as a fundamental mechanism by which cells confer a dynamic state to adhesive junctions. Recent studies indicate that the juxtamembrane domain of classical cadherins contains multiple endocytic motifs, or "switches," that can be used by cellular membrane trafficking machinery to regulate adhesion.The cadherin-binding protein p120-catenin (p120) appears to be the master regulator of access to these switches, thereby controlling cadherin endocytosis and turnover. This review focuses on p120 and other cadherin-binding proteins, ubiquitin ligases, and growth factors as key modulators of cadherin membrane trafficking. K E Y W O R D Sadherens junction, adhesion, cadherin, desmosome, endocytosis, p120-catenin, recycling

show abstract

Cadherin-11 endocytosis through binding to clathrin promotes cadherin-11-mediated migration in prostate cancer cells

Cited by 22 publications

References 46 publications

Combined overexpression of cadherin 6, cadherin 11 and cluster of differentiation 44 is associated with lymph node metastasis and poor prognosis in oral squamous cell carcinoma

Combined overexpression of cadherin 6, cadherin 11 and cluster of differentiation 44 is associated with lymph node metastasis and poor prognosis in oral squamous cell carcinoma

Dynamic changes in the urine proteome of tumor-bearing mouse models of B16 melanoma and RM-1 prostate cancer

Cadherin tales: Regulation of cadherin function by endocytic membrane trafficking

Contact Info

Product

Resources

About